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imagequant data analysis software  (Danaher Inc)


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    Structured Review

    Danaher Inc imagequant data analysis software
    Imagequant Data Analysis Software, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagequant+data+analysis+software/us12516375-1245-66-70
    Average 86 stars, based on 1 article reviews
    imagequant data analysis software - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Western Blot:

    Article Title: Maternal high fat–high energy diet alters metabolic factors in the non‐human primate fetal heart
    Article Snippet: Enhanced chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific) was used to detect the bands on the blots. .. The western blot was imaged using ImageQuant LAS 4000 (GE Healthcare Life sciences) and the protein abundance was quantified by densitometry using ImageQuant analysis software (GE Healthcare Life Sciences) and normalised to either β-actin or Ponceau S (total protein stain) as previously described (Darby et al., 2018; Lie et al., 2014; Soo et al., 2012). .. Due to the amount of frozen tissue available, tissue hormone (cortisol, cortisone, 11-deoxycortisone, triiodothyronine (T3) and thyroxine (T4)) concentrations in a subset of animals (control diet, n= 2 F, 5M;HF-HED, n = 5 F, 6 M) were determined by liquid chromatography (LC; Shimadzu Nexera XR, Shimadzu, Japan) coupled to a SCIEX 6500 Triple-Quad system (MS/MS; SCIEX, Framingham, MA, USA) using an adapted protocol (Dimasi et al., 2023b; Lock et al., 2023; McBride et al., 2020).

    Quantitative Proteomics:

    Article Title: Maternal high fat–high energy diet alters metabolic factors in the non‐human primate fetal heart
    Article Snippet: Enhanced chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific) was used to detect the bands on the blots. .. The western blot was imaged using ImageQuant LAS 4000 (GE Healthcare Life sciences) and the protein abundance was quantified by densitometry using ImageQuant analysis software (GE Healthcare Life Sciences) and normalised to either β-actin or Ponceau S (total protein stain) as previously described (Darby et al., 2018; Lie et al., 2014; Soo et al., 2012). .. Due to the amount of frozen tissue available, tissue hormone (cortisol, cortisone, 11-deoxycortisone, triiodothyronine (T3) and thyroxine (T4)) concentrations in a subset of animals (control diet, n= 2 F, 5M;HF-HED, n = 5 F, 6 M) were determined by liquid chromatography (LC; Shimadzu Nexera XR, Shimadzu, Japan) coupled to a SCIEX 6500 Triple-Quad system (MS/MS; SCIEX, Framingham, MA, USA) using an adapted protocol (Dimasi et al., 2023b; Lock et al., 2023; McBride et al., 2020).

    Software:

    Article Title: Maternal high fat–high energy diet alters metabolic factors in the non‐human primate fetal heart
    Article Snippet: Enhanced chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific) was used to detect the bands on the blots. .. The western blot was imaged using ImageQuant LAS 4000 (GE Healthcare Life sciences) and the protein abundance was quantified by densitometry using ImageQuant analysis software (GE Healthcare Life Sciences) and normalised to either β-actin or Ponceau S (total protein stain) as previously described (Darby et al., 2018; Lie et al., 2014; Soo et al., 2012). .. Due to the amount of frozen tissue available, tissue hormone (cortisol, cortisone, 11-deoxycortisone, triiodothyronine (T3) and thyroxine (T4)) concentrations in a subset of animals (control diet, n= 2 F, 5M;HF-HED, n = 5 F, 6 M) were determined by liquid chromatography (LC; Shimadzu Nexera XR, Shimadzu, Japan) coupled to a SCIEX 6500 Triple-Quad system (MS/MS; SCIEX, Framingham, MA, USA) using an adapted protocol (Dimasi et al., 2023b; Lock et al., 2023; McBride et al., 2020).

    Article Title: Compositions, devices, systems, and methods for using a nanopore
    Article Snippet: .. After incubation at 65° C. for 18 hours, the hybridization buffer is removed, and the substrate is washed sequentially under increasingly stringent conditions, up to 40 mM sodium phosphate, 1% SDS, 1 mM EDTA at 65° C. To detect signal produced by a radiolabeled probe hybridized on a membrane, the substrate is exposed to a PHOSPHORIMAGER cassette (Amersham Pharmacia Biotech), and the image is analyzed using IMAGEQUANT data analysis software (Amersham Pharmacia Biotech). ..

    Article Title: Sae2 controls Mre11 endo- and exonuclease activities by different mechanisms
    Article Snippet: Probes were labeled by Klenow fragment (New England Biolabs) with [α- 32 P]dATP (PerkinElmer) after annealing with random hexanucleotides (New England Biolabs). .. 32 P signals were detected and analyzed by a phosphorimager Typhoon FLA7000 using ImageQuant TL software (Cytiva). .. Spo11-oligos were immunoprecipitated from yeast SK1 strains containing the SPO11-FLAG allele as described , .

    Article Title: Plants with increased water use efficiency
    Article Snippet: Following primary antibody labeling, the samples were labeled with secondary antibody (W401B, Promega) at a dilution of 1:2,500. .. Last, the total protein in each labeled band was quantified using densitometry (ImageQuant LAS-4010, GE Healthcare Life Sciences) with ImageQuant TL software (Version 7.0 GE Healthcare Life Sciences). ..

    Article Title: SOX2 interacts with hnRNPK to modulate alternative splicing in mouse embryonic stem cells
    Article Snippet: .. Subsequently, the reaction samples were separated on an 8% native polyacrylamide gel in darkness at 4 °C in 0.5 × TAE at 200 V. Signal visualization was performed using a TyphoonTM FLA 7000 biomolecular imager (GE Healthcare), and quantification was carried out using the ImageQuant TL software (GE Healthcare). ..

    Article Title: A virally-encoded tRNA neutralizes the PARIS antiviral defence system.
    Article Snippet: Nathaniel Burman, Svetlana Belukhina, Florence Depardieu, Royce A. Wilkinson, Mikhail Skutel, Andrew Santiago-Frangos, Ava B. Graham, Alexei Livenskyi, Anna Chechenina, Natalia Morozova, Trevor Z ah l , W il li am S. Henriques, Murat Buyukyoruk, Christophe Rouillon, Baptiste Saudemont, Lena Shyrokova, Tatsuaki Kurata, Vasili H au ry li uk , K on stantin Severinov, Justine Groseille, Agnès Thierry, Romain Koszul, Florian Tesson, Aude Bernheim, David Bikard, Blake Wiedenheft & Artem Isaev

    Article Title: Structural and biochemical characterization of the C‐terminal region of the human RTEL 1 helicase
    Article Snippet: .. Fluorescent labeled substrates were detected by fluorescent scanner (ImageQuant, GE Healthcare) and quantification of protein bound nucleic acid was performed with ImageQuant image analysis software (GE Healthcare). .. Each experiment has been repeated three times and plotted using Prism 8 (Graphpad).

    Article Title: Structural basis for transthiolation intermediates in the ubiquitin pathway
    Article Snippet: To increase fluorescence signal by converting fluorescein to its di-anionic form, gels were incubated in 50 mM Tris•HCl pH 9.5 for 3 min prior to scanning using Amersham Typhoon 5 (Cytiva). .. Bands were quantified using ImageQuant software (Cytiva). ..

    Staining:

    Article Title: Maternal high fat–high energy diet alters metabolic factors in the non‐human primate fetal heart
    Article Snippet: Enhanced chemiluminescence using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific) was used to detect the bands on the blots. .. The western blot was imaged using ImageQuant LAS 4000 (GE Healthcare Life sciences) and the protein abundance was quantified by densitometry using ImageQuant analysis software (GE Healthcare Life Sciences) and normalised to either β-actin or Ponceau S (total protein stain) as previously described (Darby et al., 2018; Lie et al., 2014; Soo et al., 2012). .. Due to the amount of frozen tissue available, tissue hormone (cortisol, cortisone, 11-deoxycortisone, triiodothyronine (T3) and thyroxine (T4)) concentrations in a subset of animals (control diet, n= 2 F, 5M;HF-HED, n = 5 F, 6 M) were determined by liquid chromatography (LC; Shimadzu Nexera XR, Shimadzu, Japan) coupled to a SCIEX 6500 Triple-Quad system (MS/MS; SCIEX, Framingham, MA, USA) using an adapted protocol (Dimasi et al., 2023b; Lock et al., 2023; McBride et al., 2020).

    Incubation:

    Article Title: Compositions, devices, systems, and methods for using a nanopore
    Article Snippet: .. After incubation at 65° C. for 18 hours, the hybridization buffer is removed, and the substrate is washed sequentially under increasingly stringent conditions, up to 40 mM sodium phosphate, 1% SDS, 1 mM EDTA at 65° C. To detect signal produced by a radiolabeled probe hybridized on a membrane, the substrate is exposed to a PHOSPHORIMAGER cassette (Amersham Pharmacia Biotech), and the image is analyzed using IMAGEQUANT data analysis software (Amersham Pharmacia Biotech). ..

    Hybridization:

    Article Title: Compositions, devices, systems, and methods for using a nanopore
    Article Snippet: .. After incubation at 65° C. for 18 hours, the hybridization buffer is removed, and the substrate is washed sequentially under increasingly stringent conditions, up to 40 mM sodium phosphate, 1% SDS, 1 mM EDTA at 65° C. To detect signal produced by a radiolabeled probe hybridized on a membrane, the substrate is exposed to a PHOSPHORIMAGER cassette (Amersham Pharmacia Biotech), and the image is analyzed using IMAGEQUANT data analysis software (Amersham Pharmacia Biotech). ..

    Produced:

    Article Title: Compositions, devices, systems, and methods for using a nanopore
    Article Snippet: .. After incubation at 65° C. for 18 hours, the hybridization buffer is removed, and the substrate is washed sequentially under increasingly stringent conditions, up to 40 mM sodium phosphate, 1% SDS, 1 mM EDTA at 65° C. To detect signal produced by a radiolabeled probe hybridized on a membrane, the substrate is exposed to a PHOSPHORIMAGER cassette (Amersham Pharmacia Biotech), and the image is analyzed using IMAGEQUANT data analysis software (Amersham Pharmacia Biotech). ..

    Membrane:

    Article Title: Compositions, devices, systems, and methods for using a nanopore
    Article Snippet: .. After incubation at 65° C. for 18 hours, the hybridization buffer is removed, and the substrate is washed sequentially under increasingly stringent conditions, up to 40 mM sodium phosphate, 1% SDS, 1 mM EDTA at 65° C. To detect signal produced by a radiolabeled probe hybridized on a membrane, the substrate is exposed to a PHOSPHORIMAGER cassette (Amersham Pharmacia Biotech), and the image is analyzed using IMAGEQUANT data analysis software (Amersham Pharmacia Biotech). ..

    Labeling:

    Article Title: Plants with increased water use efficiency
    Article Snippet: Following primary antibody labeling, the samples were labeled with secondary antibody (W401B, Promega) at a dilution of 1:2,500. .. Last, the total protein in each labeled band was quantified using densitometry (ImageQuant LAS-4010, GE Healthcare Life Sciences) with ImageQuant TL software (Version 7.0 GE Healthcare Life Sciences). ..

    Article Title: Structural and biochemical characterization of the C‐terminal region of the human RTEL 1 helicase
    Article Snippet: .. Fluorescent labeled substrates were detected by fluorescent scanner (ImageQuant, GE Healthcare) and quantification of protein bound nucleic acid was performed with ImageQuant image analysis software (GE Healthcare). .. Each experiment has been repeated three times and plotted using Prism 8 (Graphpad).

    Thin Layer Chromatography:

    Article Title: A virally-encoded tRNA neutralizes the PARIS antiviral defence system.
    Article Snippet: Nathaniel Burman, Svetlana Belukhina, Florence Depardieu, Royce A. Wilkinson, Mikhail Skutel, Andrew Santiago-Frangos, Ava B. Graham, Alexei Livenskyi, Anna Chechenina, Natalia Morozova, Trevor Z ah l , W il li am S. Henriques, Murat Buyukyoruk, Christophe Rouillon, Baptiste Saudemont, Lena Shyrokova, Tatsuaki Kurata, Vasili H au ry li uk , K on stantin Severinov, Justine Groseille, Agnès Thierry, Romain Koszul, Florian Tesson, Aude Bernheim, David Bikard, Blake Wiedenheft & Artem Isaev



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    Effect of CADO and PGE2 on cAMP production (a) and CREB (b) and Csk (c) phosphorylation. a TILs #2009 at stage II were incubated with CADO (50 μM) and/or PGE2 (0.5 μM) or CGS (50 μM) and/or butaprost (5 μM) for 10 min. The levels of cAMP in cellular extracts were analyzed using mass spectrometry. b, c TILs #2009 at stage II were stimulated with anti-CD3 in the presence of CADO (50 μM) and/or PGE2 (0.5 μM) for 30 min. TIL protein extracts (50 μg) were resolved using 10% SDS-PAGE and transferred to PVDF membranes. The level of CREB (b) and Csk (c) phosphorylation was analyzed using specific antibodies. The optic density (OD) ratio of the bands was analyzed using <t>ImageQuanT</t> data analysis software (Molecular Dynamics)
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    Image Search Results


    Effect of CADO and PGE2 on cAMP production (a) and CREB (b) and Csk (c) phosphorylation. a TILs #2009 at stage II were incubated with CADO (50 μM) and/or PGE2 (0.5 μM) or CGS (50 μM) and/or butaprost (5 μM) for 10 min. The levels of cAMP in cellular extracts were analyzed using mass spectrometry. b, c TILs #2009 at stage II were stimulated with anti-CD3 in the presence of CADO (50 μM) and/or PGE2 (0.5 μM) for 30 min. TIL protein extracts (50 μg) were resolved using 10% SDS-PAGE and transferred to PVDF membranes. The level of CREB (b) and Csk (c) phosphorylation was analyzed using specific antibodies. The optic density (OD) ratio of the bands was analyzed using ImageQuanT data analysis software (Molecular Dynamics)

    Journal: Cancer immunology, immunotherapy : CII

    Article Title: Receptor desensitization and blockade of the suppressive effects of prostaglandin E 2 and adenosine on the cytotoxic activity of human melanoma-infiltrating T lymphocytes

    doi: 10.1007/s00262-010-0924-z

    Figure Lengend Snippet: Effect of CADO and PGE2 on cAMP production (a) and CREB (b) and Csk (c) phosphorylation. a TILs #2009 at stage II were incubated with CADO (50 μM) and/or PGE2 (0.5 μM) or CGS (50 μM) and/or butaprost (5 μM) for 10 min. The levels of cAMP in cellular extracts were analyzed using mass spectrometry. b, c TILs #2009 at stage II were stimulated with anti-CD3 in the presence of CADO (50 μM) and/or PGE2 (0.5 μM) for 30 min. TIL protein extracts (50 μg) were resolved using 10% SDS-PAGE and transferred to PVDF membranes. The level of CREB (b) and Csk (c) phosphorylation was analyzed using specific antibodies. The optic density (OD) ratio of the bands was analyzed using ImageQuanT data analysis software (Molecular Dynamics)

    Article Snippet: Films were scanned and analyzed by ImageQuanT data analysis software (Molecular Dynamics) [ 17 ].

    Techniques: Phospho-proteomics, Incubation, Mass Spectrometry, SDS Page, Software