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insulinoma antigen 2 (ia-2)853–872 peptide  (Proimmune)

 
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    Structured Review

    Proimmune insulinoma antigen 2 (ia-2)853–872 peptide
    Insulinoma Antigen 2 (Ia 2)853–872 Peptide, supplied by Proimmune, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ia-2-peptide/insulinoma+antigen+2++ia+2+853+872+peptide/pm28131926-92-25-30
    Average 90 stars, based on 1 article reviews
    insulinoma antigen 2 (ia-2)853–872 peptide - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    In Vitro:

    Article Title: General immune dampening is associated with disturbed metabolism at diagnosis of type 1 diabetes.
    Article Snippet: In Vitro Cell Culturing Aliquots of one million PBMCs (~91% viability for each population) were diluted in 1,000 μl AIM V research-grade serum-free medium (Gibco; Invitrogen, Stockholm, Sweden) supplemented with 2 mmol/l l-glutamine, 50 μg/l streptomycin sulfate, 10 μg/l gentamicin sulfate, and 2 × 10−5 mol/l 2-mercaptoethanol (Sigma, Stockholm, Sweden) and incubated in medium alone (spontaneous secretion) or stimulated with 5 μg/ml of the autoantigens GAD65 (protein; Diamyd Diagnostics AB, Stockholm, Sweden), the synthetic peptide of GAD65 AA 247–279 (NMYAMMIARFKMFPEVKEKGMAALPRLIAFTSE-OH) with molecular weight 3823.7 (Department of Medical and Physiological Chemistry, University of Uppsala, Sweden), a peptide of heat-shock protein 60 AA 437–460 (DiaPep277, a gift from B. Roep, Leiden University Medical Center, Leiden, The Netherlands) and the mitogen PHA (Sigma), 0.5 μg/ml insulin peptide (AA 9–23, Sigma, Genosys, Canada), and 10 μg/ml IA-2-peptide (AA 853–872, Proimmune, Oxford, UK) (order of priority in case of inadequate cell count; spontaneous, GAD65-protein, IA-2-peptide, heat-shock protein 60-peptide, insulin-peptide, GAD65-peptide, and finally PHA) at 37 °C in a humidified atmosphere with 5% CO2 (14,19,20).

    Cell Culture:

    Article Title: General immune dampening is associated with disturbed metabolism at diagnosis of type 1 diabetes.
    Article Snippet: In Vitro Cell Culturing Aliquots of one million PBMCs (~91% viability for each population) were diluted in 1,000 μl AIM V research-grade serum-free medium (Gibco; Invitrogen, Stockholm, Sweden) supplemented with 2 mmol/l l-glutamine, 50 μg/l streptomycin sulfate, 10 μg/l gentamicin sulfate, and 2 × 10−5 mol/l 2-mercaptoethanol (Sigma, Stockholm, Sweden) and incubated in medium alone (spontaneous secretion) or stimulated with 5 μg/ml of the autoantigens GAD65 (protein; Diamyd Diagnostics AB, Stockholm, Sweden), the synthetic peptide of GAD65 AA 247–279 (NMYAMMIARFKMFPEVKEKGMAALPRLIAFTSE-OH) with molecular weight 3823.7 (Department of Medical and Physiological Chemistry, University of Uppsala, Sweden), a peptide of heat-shock protein 60 AA 437–460 (DiaPep277, a gift from B. Roep, Leiden University Medical Center, Leiden, The Netherlands) and the mitogen PHA (Sigma), 0.5 μg/ml insulin peptide (AA 9–23, Sigma, Genosys, Canada), and 10 μg/ml IA-2-peptide (AA 853–872, Proimmune, Oxford, UK) (order of priority in case of inadequate cell count; spontaneous, GAD65-protein, IA-2-peptide, heat-shock protein 60-peptide, insulin-peptide, GAD65-peptide, and finally PHA) at 37 °C in a humidified atmosphere with 5% CO2 (14,19,20).

    Incubation:

    Article Title: General immune dampening is associated with disturbed metabolism at diagnosis of type 1 diabetes.
    Article Snippet: In Vitro Cell Culturing Aliquots of one million PBMCs (~91% viability for each population) were diluted in 1,000 μl AIM V research-grade serum-free medium (Gibco; Invitrogen, Stockholm, Sweden) supplemented with 2 mmol/l l-glutamine, 50 μg/l streptomycin sulfate, 10 μg/l gentamicin sulfate, and 2 × 10−5 mol/l 2-mercaptoethanol (Sigma, Stockholm, Sweden) and incubated in medium alone (spontaneous secretion) or stimulated with 5 μg/ml of the autoantigens GAD65 (protein; Diamyd Diagnostics AB, Stockholm, Sweden), the synthetic peptide of GAD65 AA 247–279 (NMYAMMIARFKMFPEVKEKGMAALPRLIAFTSE-OH) with molecular weight 3823.7 (Department of Medical and Physiological Chemistry, University of Uppsala, Sweden), a peptide of heat-shock protein 60 AA 437–460 (DiaPep277, a gift from B. Roep, Leiden University Medical Center, Leiden, The Netherlands) and the mitogen PHA (Sigma), 0.5 μg/ml insulin peptide (AA 9–23, Sigma, Genosys, Canada), and 10 μg/ml IA-2-peptide (AA 853–872, Proimmune, Oxford, UK) (order of priority in case of inadequate cell count; spontaneous, GAD65-protein, IA-2-peptide, heat-shock protein 60-peptide, insulin-peptide, GAD65-peptide, and finally PHA) at 37 °C in a humidified atmosphere with 5% CO2 (14,19,20).

    Molecular Weight:

    Article Title: General immune dampening is associated with disturbed metabolism at diagnosis of type 1 diabetes.
    Article Snippet: In Vitro Cell Culturing Aliquots of one million PBMCs (~91% viability for each population) were diluted in 1,000 μl AIM V research-grade serum-free medium (Gibco; Invitrogen, Stockholm, Sweden) supplemented with 2 mmol/l l-glutamine, 50 μg/l streptomycin sulfate, 10 μg/l gentamicin sulfate, and 2 × 10−5 mol/l 2-mercaptoethanol (Sigma, Stockholm, Sweden) and incubated in medium alone (spontaneous secretion) or stimulated with 5 μg/ml of the autoantigens GAD65 (protein; Diamyd Diagnostics AB, Stockholm, Sweden), the synthetic peptide of GAD65 AA 247–279 (NMYAMMIARFKMFPEVKEKGMAALPRLIAFTSE-OH) with molecular weight 3823.7 (Department of Medical and Physiological Chemistry, University of Uppsala, Sweden), a peptide of heat-shock protein 60 AA 437–460 (DiaPep277, a gift from B. Roep, Leiden University Medical Center, Leiden, The Netherlands) and the mitogen PHA (Sigma), 0.5 μg/ml insulin peptide (AA 9–23, Sigma, Genosys, Canada), and 10 μg/ml IA-2-peptide (AA 853–872, Proimmune, Oxford, UK) (order of priority in case of inadequate cell count; spontaneous, GAD65-protein, IA-2-peptide, heat-shock protein 60-peptide, insulin-peptide, GAD65-peptide, and finally PHA) at 37 °C in a humidified atmosphere with 5% CO2 (14,19,20).

    Cell Counting:

    Article Title: General immune dampening is associated with disturbed metabolism at diagnosis of type 1 diabetes.
    Article Snippet: In Vitro Cell Culturing Aliquots of one million PBMCs (~91% viability for each population) were diluted in 1,000 μl AIM V research-grade serum-free medium (Gibco; Invitrogen, Stockholm, Sweden) supplemented with 2 mmol/l l-glutamine, 50 μg/l streptomycin sulfate, 10 μg/l gentamicin sulfate, and 2 × 10−5 mol/l 2-mercaptoethanol (Sigma, Stockholm, Sweden) and incubated in medium alone (spontaneous secretion) or stimulated with 5 μg/ml of the autoantigens GAD65 (protein; Diamyd Diagnostics AB, Stockholm, Sweden), the synthetic peptide of GAD65 AA 247–279 (NMYAMMIARFKMFPEVKEKGMAALPRLIAFTSE-OH) with molecular weight 3823.7 (Department of Medical and Physiological Chemistry, University of Uppsala, Sweden), a peptide of heat-shock protein 60 AA 437–460 (DiaPep277, a gift from B. Roep, Leiden University Medical Center, Leiden, The Netherlands) and the mitogen PHA (Sigma), 0.5 μg/ml insulin peptide (AA 9–23, Sigma, Genosys, Canada), and 10 μg/ml IA-2-peptide (AA 853–872, Proimmune, Oxford, UK) (order of priority in case of inadequate cell count; spontaneous, GAD65-protein, IA-2-peptide, heat-shock protein 60-peptide, insulin-peptide, GAD65-peptide, and finally PHA) at 37 °C in a humidified atmosphere with 5% CO2 (14,19,20).



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