human umbilical vein endothelial cells huvecs  (ATCC)

Journal: Materials Today Bio

Article Title: Procyanidin capsules attenuate PI3K/AKT-mediated mitochondrial dysfunction and accelerate skin wound healing in diabetic mice

doi: 10.1016/j.mtbio.2026.103029

Figure Lengend Snippet: Biocompatibility of PC capsules: (A–B) cell viability of HUVECs (A) and NIH/3T3 cells (B) treated with different concentrations of PC capsules for 24 h detected by MTT assay; (C–D) toxic staining of PC capsules on HUVECs and NIH/3T3 cells detected by living/dead cell staining; (E) photos of the backs of mice after receiving a subcutaneous injection of PC capsules and subcutaneous PC capsules after injection at day 4 and day 7; (F) slices of major organs (heart, liver, spleen, lungs, and kidneys) in mice after 14 days of PC capsule administration; scale bar = 100 μm. Serum liver function indicators were: (G) ALT and (H) AST. Serum renal function indicators were: (I) BUN and (J) CRE. Data are presented as mean ± standard deviation (SD) with n = 3 independent biological replicates per group. Statistical analysis was performed using one-way ANOVA followed by Tukey's post-hoc test. Significance markers are defined as: ns (p > 0.05).

Article Snippet: Human umbilical vein endothelial cells (HUVECs) (CRL-1730, USA, RRID: CVCL_2959) and NIH/3T3 cells (CRL-1658, USA, RRID: CVCL_0594) used in this study were purchased from American Type Culture Collection.

Techniques: Capsules, MTT Assay, Staining, Injection, Standard Deviation

Journal: Materials Today Bio

Article Title: Procyanidin capsules attenuate PI3K/AKT-mediated mitochondrial dysfunction and accelerate skin wound healing in diabetic mice

doi: 10.1016/j.mtbio.2026.103029

Figure Lengend Snippet: PC capsules increased cell viability under H 2 O 2 stimulation: cell viability of HUVECs (A) and NIH/3T3 (B) cells treated with different concentrations of H 2 O 2 for 6 h detected by MTT assay; viability of H 2 O 2 ‐treated HUVECs (C) and NIH/3T3 cells (D) with different concentrations of PC capsule pretreatment. Data are presented as mean ± standard deviation (SD) with n = 3 independent biological replicates per group. Statistical analysis was performed using one-way ANOVA followed by Tukey's post-hoc test. Significance markers are defined as: ∗∗∗∗p < 0.0001.

Article Snippet: Human umbilical vein endothelial cells (HUVECs) (CRL-1730, USA, RRID: CVCL_2959) and NIH/3T3 cells (CRL-1658, USA, RRID: CVCL_0594) used in this study were purchased from American Type Culture Collection.

Techniques: Capsules, MTT Assay, Standard Deviation

Journal: Materials Today Bio

Article Title: Procyanidin capsules attenuate PI3K/AKT-mediated mitochondrial dysfunction and accelerate skin wound healing in diabetic mice

doi: 10.1016/j.mtbio.2026.103029

Figure Lengend Snippet: PC capsules restored cell function in H 2 O 2 -treated HUVECs and NIH/3T3 cells. HUVECs and NIH/3T3 cells were treated with PC capsules for 24 h and H 2 O 2 for 6 h. (A) Images of HUVEC migration exposure to H 2 O 2 , NAC, PC capsules after 24 h; (B) quantification of percentage wound area remaining for HUVECs; (C) images of NIH/3T3 cell migration exposure to H 2 O 2 , NAC, PC capsules after 24 h; (D) quantification of percentage wound area remaining for NIH/3T3 cells; (E–F) digital images of endothelial cell microtubule formation after treatment with H 2 O 2 , NAC, and PC capsules. Quantification of (G) number of branch sites, (H) total lengths and (I) numbers of tube nodes. Data are presented as mean ± standard deviation (SD) with n = 3 independent biological replicates per group. Statistical analysis was performed using one-way ANOVA followed by Tukey's post-hoc test. Significance markers are defined as: ##p < 0.01, ###p < 0.001 (vs. control group); ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (vs. H 2 O 2 group).

Article Snippet: Human umbilical vein endothelial cells (HUVECs) (CRL-1730, USA, RRID: CVCL_2959) and NIH/3T3 cells (CRL-1658, USA, RRID: CVCL_0594) used in this study were purchased from American Type Culture Collection.

Techniques: Capsules, Cell Function Assay, Migration, Standard Deviation, Control

Journal: Materials Today Bio

Article Title: Procyanidin capsules attenuate PI3K/AKT-mediated mitochondrial dysfunction and accelerate skin wound healing in diabetic mice

doi: 10.1016/j.mtbio.2026.103029

Figure Lengend Snippet: PC capsules improved mitochondrial function in H 2 O 2 -treated HUVECs and NIH/3T3 cells: (A–D) elimination of ROS from HUVECs (A, C) and NIH/3T3 (B, D) cells by PC capsules as determined by MitoSOX; (E–H) assessment of PC-mediated HUVECs (E, G) and NIH/3T3 cells (F, H) MMP using TMRM assays; (I–J) effects of PC capsules on ATP production in HUVECs (I) and NIH/3T3 cells (J). Data are presented as mean ± standard deviation (SD) with n = 3 independent biological replicates per group. Statistical analysis was performed using one-way ANOVA followed by Tukey's post-hoc test. Significance markers are defined as: ##p < 0.01, ###p < 0.001, ####p < 0.0001 (vs. control group); ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 (vs. H 2 O 2 group).

Article Snippet: Human umbilical vein endothelial cells (HUVECs) (CRL-1730, USA, RRID: CVCL_2959) and NIH/3T3 cells (CRL-1658, USA, RRID: CVCL_0594) used in this study were purchased from American Type Culture Collection.

Techniques: Capsules, Standard Deviation, Control

Journal: Materials Today Bio

Article Title: Procyanidin capsules attenuate PI3K/AKT-mediated mitochondrial dysfunction and accelerate skin wound healing in diabetic mice

doi: 10.1016/j.mtbio.2026.103029

Figure Lengend Snippet: PC capsule–mediated cytoprotection via promoting activation of PI3K/AKT signaling pathway: expression of p-PI3K, PI3K, p-AKT, and AKT in HUVECs treated with PC capsules for 24 h and presence/absence of H 2 O 2 for 6 h (A); and analysis of optical density values of p-PI3K/PI3K (B) and p-AKT/AKT (C); expression of p-PI3K, PI3K, p-AKT, and AKT in NIH/3T3 cells treated with PC capsules for 24 h and presence/absence of H 2 O 2 for 6 h (D); and analysis of optical density values of p-PI3K/PI3K (E) and p-AKT/AKT (F). Data are presented as mean ± standard deviation (SD) with n = 3 independent biological replicates per group. Statistical analysis was performed using one-way ANOVA followed by Tukey's post-hoc test. Significance markers are defined as: #p < 0.05, ##p < 0.01, ###p < 0.001 (vs control group); ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (vs PC group or vs H 2 O 2 group).

Article Snippet: Human umbilical vein endothelial cells (HUVECs) (CRL-1730, USA, RRID: CVCL_2959) and NIH/3T3 cells (CRL-1658, USA, RRID: CVCL_0594) used in this study were purchased from American Type Culture Collection.

Techniques: Activation Assay, Expressing, Capsules, Standard Deviation, Control

Journal: Materials Today Bio

Article Title: Procyanidin capsules attenuate PI3K/AKT-mediated mitochondrial dysfunction and accelerate skin wound healing in diabetic mice

doi: 10.1016/j.mtbio.2026.103029

Figure Lengend Snippet: PC capsules improve HUVEC and NIH/3T3 cell dysfunction by regulating PI3K/AKT signaling. HUVECs and NIH/3T3 cells were treated with PC capsules and LY294002 for 24 h and H 2 O 2 for 6 h. (A) Images of HUVEC migration exposure to H 2 O 2 , LY294002, PC capsules after 24 h; (B) quantification of percentage wound area remaining for HUVECs; (C) images of NIH/3T3 cell migration exposure to H 2 O 2 , LY294002, PC capsules after 24 h; (D) quantification of percentage wound area remaining for NIH/3T3 cells; (E–F) digital images of endothelial cell microtubule formation after treatment with H 2 O 2 , LY294002, and PC capsules. Quantification of (G) number of branch sites, (H) total lengths and (I) numbers of tube nodes (J); and analysis of optical density values of p-PI3K/PI3K (K) and p-AKT/AKT (L); expression of p-PI3K, PI3K, p-AKT, and AKT in NIH/3T3 cells treated with PC capsules, and LY294002 for 24 h and presence/absence of H 2 O 2 for 6 h (M); and analysis of optical density values of p-PI3K/PI3K (N) and p-AKT/AKT (O). Data are presented as mean ± standard deviation (SD) with n = 3 independent biological replicates per group. Statistical analysis was performed using one-way ANOVA followed by Tukey's post-hoc test. Significance markers are defined as: ##p < 0.01, ###p < 0.001 (vs. control group); ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (vs PC group or vs LY294002 group).

Article Snippet: Human umbilical vein endothelial cells (HUVECs) (CRL-1730, USA, RRID: CVCL_2959) and NIH/3T3 cells (CRL-1658, USA, RRID: CVCL_0594) used in this study were purchased from American Type Culture Collection.

Techniques: Capsules, Migration, Expressing, Standard Deviation, Control

Journal: bioRxiv

Article Title: Chlamydia trachomatis infection upregulates MicroRNA21 to deplete tumor suppressor PTEN

doi: 10.64898/2026.05.11.723860

Figure Lengend Snippet: Chlamydia trachomatis infection leads to up regulation of oncomiR, miR21 to deplete PTEN level. A. Hela229 cells were infected with heat killed or infectious Ct for different (early) time points and miR21 levels were detected by northern blotting. U6 was detected as loading control. B. Hela229 cells were left uninfected (UI) or infected with infectious Ct for different time points. The total RNA was used to perform northern blotting to detect levels of miR21. U6 serves as loading control and Ct R5 is the infection control. n=3. C. Human Fimb and Huvec cells were infected with infectious Ct for 30 h and the total RNA was used for Northern blot analysis to detect the levels of miR21; UI-uninfected, Ct R5-infection control and U6 is the loading control. D. HeLa229 cells were either left uninfected or infected with Ct for different time points. The total RNA was isolated and quantitative RT-PCR was used to quantify the levels of miR21. Three independent experiments were performed with the mean values (±SEM) compared to levels of uninfected HeLa229 cells. Statistical analysis was performed using Student t test (**p ≤ 0.01). E. HeLa229 cells were treated with scrambled RNA or antagomiR against miR21 or F over expressed with scramble RNA or miR21 RNA. The cells were left uninfected or infected with Ct for 36h and lysed for western blot analysis. The data provided is the representative of three independent experiments. n=3. G. The cells (uninfected and infected) from E (primary infection) were lysed to re-infect freshly plated HeLa229 cells to analyze the progeny, secondary infection. For the above experiments F . and G . Beta actin serves as the loading control and cHSP60, Chlamydial HSP60, detects the rate of infection. Fc shows the fold change in the levels of protein compared to UI, normalized to levels of beta actin. The data provided is the representative of three independent experiments. n=3.

Article Snippet: HeLa229 (ATCC ® CCL-2.1 TM ), human Fimb: epithelial cells isolated from fimbriae of healthy donors after hysterectomy and Huvec cells (ATCC ® CRL-1730 TM ) were used in the experiments.

Techniques: Infection, Northern Blot, Control, Isolation, Quantitative RT-PCR, Western Blot

Journal: Research

Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells

doi: 10.34133/research.1248

Figure Lengend Snippet: Isolation and characterization of chorionic plate mesenchymal stem cell (CP-MSC)-derived sEVs. (A) Schematic showing the isolation and characterization of sEVs derived from CP-MSCs cultured under O₂ Nor , O₂ Hypo-5% , or O₂ Hypo-3% conditions. (B) TEM images showing the typical bilayer structure of sEVs Nor , sEVs Hypo-5% , and sEVs Hypo-3% ; scale bar = 100 nm. (C) Nanoparticle tracking analysis (NTA) profiles showing the size distribution and concentration of sEVs Nor , sEVs Hypo-5% , and sEVs Hypo-3% after 400-fold dilution. (D) Western blotting analysis showing the expression of CD9, CD63, TSG101, and Calnexin proteins in these 3 sEVs. (E) Confocal microscopy images showing the internalization of Dil-labeled sEVs by high-glucose human umbilical vein endothelial cells (HG-HUVECs) and high-glucose human dermal fibroblasts (HG-HDFs); scale bar = 100 μm.

Article Snippet: HUVECs (CRL-1730, ATCC, USA) were cultured in DMEM containing 10% fetal bovine serum (FBS), 1% PS, and 50 mM glucose.

Techniques: Isolation, Derivative Assay, Cell Culture, Concentration Assay, Western Blot, Expressing, Confocal Microscopy, Labeling