primary human uterine smooth muscle strain hutsmc  (ATCC)

Journal: Mediators of Inflammation

Article Title: Aster spathulifolius Maxim. Alleviates Primary Dysmenorrhea in a Mouse Model by Modulating Myometrial Contractions via NF-κB/COX-2 Pathway Inhibition

doi: 10.1155/mi/1654087

Figure Lengend Snippet: Inhibitory effect of PDR97 on NF-κB activation in HUtSMCs. (A) NF-κB luciferase activity. HUtSMCs were pretreated with PDR97 for 30 min, followed by coincubation with PGF2α (10 µM) and IL-1β (10 ng/mL) for 16 h. (B) Translocation of NF-κB from the cytoplasm to the nucleus. HUtSMCs were pretreated with PDR97 for 30 min, followed by coincubation with PGF2α (10 µM) and IL-1β (10 ng/mL) for 30 min. (C) Analysis of NF-κB protein levels in the cytoplasmic and nuclear fractions. HUtSMCs were pretreated with PDR97 for 30 min, followed by coincubation with PGF2α (10 µM) and IL-1β (10 ng/mL) for 30 min. (D) Quantification of NF-κB protein levels. The relative expression level of NF-κB protein was normalized to the PGF2α- and IL-1β-treated control group. Data are presented as mean ± SEM. Statistical analysis was performed using one-way ANOVA, followed by a nonparametric test. ⁣ ∗ p < 0.05, ⁣ ∗∗ p < 0.01, ⁣ ∗∗∗ p < 0.001, ⁣ ∗∗∗∗ p < 0.0001.

Article Snippet: HUtSMCs were purchased from PromoCell (Cat# C-12575, PromoCell, Heidelberg, Germany).

Techniques: Activation Assay, Luciferase, Activity Assay, Translocation Assay, Expressing, Control