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human complement-preserved serum a113  (Quidel)


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    Structured Review

    Quidel human complement-preserved serum a113
    Human Complement Preserved Serum A113, supplied by Quidel, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+serum+complement+preserved/human+complement/us12162931-708-4-7
    Average 90 stars, based on 1 article reviews
    human complement-preserved serum a113 - by Bioz Stars, 2026-11
    90/100 stars

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    other:

    Article Title: Properdin binding protein and use thereof
    Article Snippet: Eculizumab (Targetmol, T9915), a recombinant humanized monoclonal antibody against the complement protein C5 was as a control also showed inhibitory ability in the AP pathway, with IC50 of 50 nM.

    Article Title: Properdin binding protein and use thereof
    Article Snippet: At the same time, human complement-preserved serum (Quidel, A113) was diluted to 20% vol/vol in GVBS-EGTA (1×AP buffer: 0.1% gelatin, 145 mM NaCl, 2.5 mM sodium barbital, pH7.4 with 10 mM Mg/EGTA), incubate on ice for 30 minutes and added (50 μl/well) to the rows of the same 96-well plate such that the final concentration of human serum in each well was 10%.

    Incubation:

    Article Title: Successful pharmacological intervention at different levels of the complement system in an in vitro complement fixation model for bullous pemphigoid.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense slides were incubated with 1:10 diluted normal human complement preserved serum (NHS, Quidel #A113) in HEPES buffer (0.01 M HEPES, 0.15 M NaCl, 135 nM CaCl2, 1 mM MgCl2; pH 7.4) to induce complement activation.16 All analyses were performed in duplicate and incubated with NHS alone (negative control), EDTA (positive control) or in a dose- dependent fashion in the presence of either dexamethasone (Monofree Dexamethason eyedrops, Théa Pharma, Haarlem, The Netherlands), anti- C5 monoclonal antibody (mAb) TA B L E 1 Cohort I. Pt nr. ..

    Article Title: Recruitment of Factor H to the Streptococcus suis Cell Surface is Multifactorial
    Article Snippet: .. After incubation, 450 μL of freshly thawed human serum (complement preserved, Quidel) were added to bacteria. ..

    Activation Assay:

    Article Title: Successful pharmacological intervention at different levels of the complement system in an in vitro complement fixation model for bullous pemphigoid.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense slides were incubated with 1:10 diluted normal human complement preserved serum (NHS, Quidel #A113) in HEPES buffer (0.01 M HEPES, 0.15 M NaCl, 135 nM CaCl2, 1 mM MgCl2; pH 7.4) to induce complement activation.16 All analyses were performed in duplicate and incubated with NHS alone (negative control), EDTA (positive control) or in a dose- dependent fashion in the presence of either dexamethasone (Monofree Dexamethason eyedrops, Théa Pharma, Haarlem, The Netherlands), anti- C5 monoclonal antibody (mAb) TA B L E 1 Cohort I. Pt nr. ..

    Negative Control:

    Article Title: Successful pharmacological intervention at different levels of the complement system in an in vitro complement fixation model for bullous pemphigoid.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense slides were incubated with 1:10 diluted normal human complement preserved serum (NHS, Quidel #A113) in HEPES buffer (0.01 M HEPES, 0.15 M NaCl, 135 nM CaCl2, 1 mM MgCl2; pH 7.4) to induce complement activation.16 All analyses were performed in duplicate and incubated with NHS alone (negative control), EDTA (positive control) or in a dose- dependent fashion in the presence of either dexamethasone (Monofree Dexamethason eyedrops, Théa Pharma, Haarlem, The Netherlands), anti- C5 monoclonal antibody (mAb) TA B L E 1 Cohort I. Pt nr. ..

    Positive Control:

    Article Title: Successful pharmacological intervention at different levels of the complement system in an in vitro complement fixation model for bullous pemphigoid.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense slides were incubated with 1:10 diluted normal human complement preserved serum (NHS, Quidel #A113) in HEPES buffer (0.01 M HEPES, 0.15 M NaCl, 135 nM CaCl2, 1 mM MgCl2; pH 7.4) to induce complement activation.16 All analyses were performed in duplicate and incubated with NHS alone (negative control), EDTA (positive control) or in a dose- dependent fashion in the presence of either dexamethasone (Monofree Dexamethason eyedrops, Théa Pharma, Haarlem, The Netherlands), anti- C5 monoclonal antibody (mAb) TA B L E 1 Cohort I. Pt nr. ..

    Bacteria:

    Article Title: Recruitment of Factor H to the Streptococcus suis Cell Surface is Multifactorial
    Article Snippet: .. After incubation, 450 μL of freshly thawed human serum (complement preserved, Quidel) were added to bacteria. ..



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    Complement Technology Inc complement preserved normal human serum (nhs)
    SAR443809 specifically inhibits AP without inhibiting CP or LP. SAR443809 and its corresponding IgG isotype control were tested in a series of assays to assess their ability to inhibit the different <t>complement</t> pathways. A dose-dilution series of SAR443809 (●) or IgG control antibody (○) were tested in the commercial Wieslab EIA assays for (A) AP, (C) CP, and (D) LP, in which the inhibition of MAC deposition upon pathway specific activation was assessed per manufacturer’s protocol. (B) Antibodies were also tested in AP hemolysis assay. Rabbit erythrocytes were incubated with 10% <t>NHS</t> containing Mg 2+ -EGTA in the presence of varying concentrations of either SAR443809 (●) or the isotype control (○). The extent of hemolysis was quantified using the absorbance of the released hemoglobin in the supernatant. The data reported here is a mean of 3 independent experiments ± standard deviation, for all 4 data sets.
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    SAR443809 specifically inhibits AP without inhibiting CP or LP. SAR443809 and its corresponding IgG isotype control were tested in a series of assays to assess their ability to inhibit the different complement pathways. A dose-dilution series of SAR443809 (●) or IgG control antibody (○) were tested in the commercial Wieslab EIA assays for (A) AP, (C) CP, and (D) LP, in which the inhibition of MAC deposition upon pathway specific activation was assessed per manufacturer’s protocol. (B) Antibodies were also tested in AP hemolysis assay. Rabbit erythrocytes were incubated with 10% NHS containing Mg 2+ -EGTA in the presence of varying concentrations of either SAR443809 (●) or the isotype control (○). The extent of hemolysis was quantified using the absorbance of the released hemoglobin in the supernatant. The data reported here is a mean of 3 independent experiments ± standard deviation, for all 4 data sets.

    Journal: Blood Advances

    Article Title: SAR443809: a selective inhibitor of the complement alternative pathway, targeting complement factor Bb

    doi: 10.1182/bloodadvances.2022009028

    Figure Lengend Snippet: SAR443809 specifically inhibits AP without inhibiting CP or LP. SAR443809 and its corresponding IgG isotype control were tested in a series of assays to assess their ability to inhibit the different complement pathways. A dose-dilution series of SAR443809 (●) or IgG control antibody (○) were tested in the commercial Wieslab EIA assays for (A) AP, (C) CP, and (D) LP, in which the inhibition of MAC deposition upon pathway specific activation was assessed per manufacturer’s protocol. (B) Antibodies were also tested in AP hemolysis assay. Rabbit erythrocytes were incubated with 10% NHS containing Mg 2+ -EGTA in the presence of varying concentrations of either SAR443809 (●) or the isotype control (○). The extent of hemolysis was quantified using the absorbance of the released hemoglobin in the supernatant. The data reported here is a mean of 3 independent experiments ± standard deviation, for all 4 data sets.

    Article Snippet: Complement preserved normal human serum (NHS) was sourced from Complement Technology Inc (Tyler, TX), BioIVT (Westbury, NY), or Innovative Research (Novi, MI).

    Techniques: Control, Inhibition, Activation Assay, Hemolysis Assay, Incubation, Standard Deviation