human complement-preserved serum a113 (Quidel)
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human complement-preserved serum a113
Human Complement Preserved Serum A113, supplied by Quidel, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+serum+complement+preserved/human+complement/us12162931-708-4-7
Average 90 stars, based on 1 article reviews
Human Complement Preserved Serum A113, supplied by Quidel, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+serum+complement+preserved/human+complement/us12162931-708-4-7
Average 90 stars, based on 1 article reviews
human complement-preserved serum a113 - by Bioz Stars,
2026-11
90/100 stars
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other:Article Title: Anti-human claudin 18.2 monoclonal antibody and application thereof Article Snippet: The antibody to be tested and the control antibody were diluted with the medium (RPMI1640 medium (Gibco, 11835-030)+1% FBS (Gibco, 10099-141)) to a final concentration of 50 ug/ml, and then diluted 5 times for 8 gradients, and the complement (Quidel, A113) was diluted to a final concentration of 1:50 (V/V). Article Title: Properdin binding protein and use thereof Article Snippet: Eculizumab (Targetmol, T9915), a recombinant humanized monoclonal antibody against the complement protein C5 was as a control also showed inhibitory ability in the AP pathway, with IC50 of 50 nM. Article Title: The Therapeutic Monoclonal Antibody Bamlanivimab Does Not Enhance SARS-CoV-2 Infection by FcR-Mediated Mechanisms Article Snippet: Five milliliters of complement from Article Title: Properdin binding protein and use thereof Article Snippet: At the same time, Article Title: The Therapeutic Monoclonal Antibody Bamlanivimab Does Not Enhance SARS-CoV-2 Infection by FcR-Mediated Mechanisms. Article Snippet: Preparation of Complement Five milliliters of complement from CDC Assay:Article Title: Human monocarboxylate transporter 1 antibodies and uses thereof Article Snippet: ADCC, ADCP and CDC assay plates were gently agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 1 h at 37° C. The stably transfected Jurkat V158 cells or Jurkat H131 cells were diluted to a concentration of 3×106 cells/mL and 50 IL/well was added to the respective ADCC and ADCP assay plates containing the serially diluted test samples and Raji cells, and the plate was mixed by gentle agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 4 hrs at 37° C. Pre-diluted complement from human serum (Quidel A113) was added to the CDC plate (50 μL/well) containing the serially diluted test samples and Raji cells, and the plate was mixed by gentle agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 2 h at 37° C. After incubation, the ADCC, ADCP, and CDC plates were brought to room temperature for 10 minutes followed by addition of 100 μL of One-glo Ex (E8130, Promega) to the ADCC and ADCP assay plates and the Cell-Titer Glo (G7571, Promega) to the CDC assay plates. .. ADCC, ADCP and CDC assay plates were gently agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 1 h at 37° C. The stably transfected Jurkat V158 cells or Jurkat H131 cells were diluted to a concentration of 3×106 cells/mL and 50 IL/well was added to the respective ADCC and ADCP assay plates containing the serially diluted test samples and Raji cells, and the plate was mixed by gentle agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 4 hrs at 37° C. Pre-diluted complement from Article Title: Antagonistic CD40 monoclonal antibodies and uses thereof Article Snippet: After the incubation, 100 microliters of CellTiter-Glo® (Promega, Madison, WI) was added to each well. .. Twenty-five microliters of Incubation:Article Title: Human monocarboxylate transporter 1 antibodies and uses thereof Article Snippet: ADCC, ADCP and CDC assay plates were gently agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 1 h at 37° C. The stably transfected Jurkat V158 cells or Jurkat H131 cells were diluted to a concentration of 3×106 cells/mL and 50 IL/well was added to the respective ADCC and ADCP assay plates containing the serially diluted test samples and Raji cells, and the plate was mixed by gentle agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 4 hrs at 37° C. Pre-diluted complement from human serum (Quidel A113) was added to the CDC plate (50 μL/well) containing the serially diluted test samples and Raji cells, and the plate was mixed by gentle agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 2 h at 37° C. After incubation, the ADCC, ADCP, and CDC plates were brought to room temperature for 10 minutes followed by addition of 100 μL of One-glo Ex (E8130, Promega) to the ADCC and ADCP assay plates and the Cell-Titer Glo (G7571, Promega) to the CDC assay plates. .. ADCC, ADCP and CDC assay plates were gently agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 1 h at 37° C. The stably transfected Jurkat V158 cells or Jurkat H131 cells were diluted to a concentration of 3×106 cells/mL and 50 IL/well was added to the respective ADCC and ADCP assay plates containing the serially diluted test samples and Raji cells, and the plate was mixed by gentle agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 4 hrs at 37° C. Pre-diluted complement from Article Title: Developing a human monoclonal antibody combination CRM25 to prevent rabies after exposure. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Stable Transfection:Article Title: Human monocarboxylate transporter 1 antibodies and uses thereof Article Snippet: ADCC, ADCP and CDC assay plates were gently agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 1 h at 37° C. The stably transfected Jurkat V158 cells or Jurkat H131 cells were diluted to a concentration of 3×106 cells/mL and 50 IL/well was added to the respective ADCC and ADCP assay plates containing the serially diluted test samples and Raji cells, and the plate was mixed by gentle agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 4 hrs at 37° C. Pre-diluted complement from human serum (Quidel A113) was added to the CDC plate (50 μL/well) containing the serially diluted test samples and Raji cells, and the plate was mixed by gentle agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 2 h at 37° C. After incubation, the ADCC, ADCP, and CDC plates were brought to room temperature for 10 minutes followed by addition of 100 μL of One-glo Ex (E8130, Promega) to the ADCC and ADCP assay plates and the Cell-Titer Glo (G7571, Promega) to the CDC assay plates. .. ADCC, ADCP and CDC assay plates were gently agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 1 h at 37° C. The stably transfected Jurkat V158 cells or Jurkat H131 cells were diluted to a concentration of 3×106 cells/mL and 50 IL/well was added to the respective ADCC and ADCP assay plates containing the serially diluted test samples and Raji cells, and the plate was mixed by gentle agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 4 hrs at 37° C. Pre-diluted complement from Transfection:Article Title: Human monocarboxylate transporter 1 antibodies and uses thereof Article Snippet: ADCC, ADCP and CDC assay plates were gently agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 1 h at 37° C. The stably transfected Jurkat V158 cells or Jurkat H131 cells were diluted to a concentration of 3×106 cells/mL and 50 IL/well was added to the respective ADCC and ADCP assay plates containing the serially diluted test samples and Raji cells, and the plate was mixed by gentle agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 4 hrs at 37° C. Pre-diluted complement from human serum (Quidel A113) was added to the CDC plate (50 μL/well) containing the serially diluted test samples and Raji cells, and the plate was mixed by gentle agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 2 h at 37° C. After incubation, the ADCC, ADCP, and CDC plates were brought to room temperature for 10 minutes followed by addition of 100 μL of One-glo Ex (E8130, Promega) to the ADCC and ADCP assay plates and the Cell-Titer Glo (G7571, Promega) to the CDC assay plates. .. ADCC, ADCP and CDC assay plates were gently agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 1 h at 37° C. The stably transfected Jurkat V158 cells or Jurkat H131 cells were diluted to a concentration of 3×106 cells/mL and 50 IL/well was added to the respective ADCC and ADCP assay plates containing the serially diluted test samples and Raji cells, and the plate was mixed by gentle agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 4 hrs at 37° C. Pre-diluted complement from Concentration Assay:Article Title: Human monocarboxylate transporter 1 antibodies and uses thereof Article Snippet: ADCC, ADCP and CDC assay plates were gently agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 1 h at 37° C. The stably transfected Jurkat V158 cells or Jurkat H131 cells were diluted to a concentration of 3×106 cells/mL and 50 IL/well was added to the respective ADCC and ADCP assay plates containing the serially diluted test samples and Raji cells, and the plate was mixed by gentle agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 4 hrs at 37° C. Pre-diluted complement from human serum (Quidel A113) was added to the CDC plate (50 μL/well) containing the serially diluted test samples and Raji cells, and the plate was mixed by gentle agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 2 h at 37° C. After incubation, the ADCC, ADCP, and CDC plates were brought to room temperature for 10 minutes followed by addition of 100 μL of One-glo Ex (E8130, Promega) to the ADCC and ADCP assay plates and the Cell-Titer Glo (G7571, Promega) to the CDC assay plates. .. ADCC, ADCP and CDC assay plates were gently agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 1 h at 37° C. The stably transfected Jurkat V158 cells or Jurkat H131 cells were diluted to a concentration of 3×106 cells/mL and 50 IL/well was added to the respective ADCC and ADCP assay plates containing the serially diluted test samples and Raji cells, and the plate was mixed by gentle agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 4 hrs at 37° C. Pre-diluted complement from Gentle:Article Title: Human monocarboxylate transporter 1 antibodies and uses thereof Article Snippet: ADCC, ADCP and CDC assay plates were gently agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 1 h at 37° C. The stably transfected Jurkat V158 cells or Jurkat H131 cells were diluted to a concentration of 3×106 cells/mL and 50 IL/well was added to the respective ADCC and ADCP assay plates containing the serially diluted test samples and Raji cells, and the plate was mixed by gentle agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 4 hrs at 37° C. Pre-diluted complement from human serum (Quidel A113) was added to the CDC plate (50 μL/well) containing the serially diluted test samples and Raji cells, and the plate was mixed by gentle agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 2 h at 37° C. After incubation, the ADCC, ADCP, and CDC plates were brought to room temperature for 10 minutes followed by addition of 100 μL of One-glo Ex (E8130, Promega) to the ADCC and ADCP assay plates and the Cell-Titer Glo (G7571, Promega) to the CDC assay plates. .. ADCC, ADCP and CDC assay plates were gently agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 1 h at 37° C. The stably transfected Jurkat V158 cells or Jurkat H131 cells were diluted to a concentration of 3×106 cells/mL and 50 IL/well was added to the respective ADCC and ADCP assay plates containing the serially diluted test samples and Raji cells, and the plate was mixed by gentle agitation on a plate shaker for 30 seconds at 200 rpm, then incubated for 4 hrs at 37° C. Pre-diluted complement from CCK-8 Assay:Article Title: Developing a human monoclonal antibody combination CRM25 to prevent rabies after exposure. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. |
