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filtered, complement preserved, mixed-gender pooled human serum #humansrm-0114014  (BioIVT Inc)

 
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    BioIVT Inc filtered, complement preserved, mixed-gender pooled human serum #humansrm-0114014
    Filtered, Complement Preserved, Mixed Gender Pooled Human Serum #Humansrm 0114014, supplied by BioIVT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+serum+complement+preserved/normal+complement+preserved+human+serum/pm40042652-118-1-11
    Average 90 stars, based on 1 article reviews
    filtered, complement preserved, mixed-gender pooled human serum #humansrm-0114014 - by Bioz Stars, 2026-10
    90/100 stars

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    Incubation:

    Article Title: CD47 antibodies, methods, and uses
    Article Snippet: 50,000 Wil2-S cells were plated in opaque white 96-well plates in 25 μl RPMI-1640 supplemented with 10% heat inactivated FBS and 0.1 mM NEAA (all reagents from Invitrogen). .. Upon addition of 25 μl of medium supplemented with or without antibodies cells were incubated for 30 minutes at 37° C. Subsequently, 50 μl of human serum (Bioreclamation, complement preserved) was added and cells were incubated at 37° C. for 2 hours. ..



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    SAR443809 specifically inhibits AP without inhibiting CP or LP. SAR443809 and its corresponding IgG isotype control were tested in a series of assays to assess their ability to inhibit the different <t>complement</t> pathways. A dose-dilution series of SAR443809 (●) or IgG control antibody (○) were tested in the commercial Wieslab EIA assays for (A) AP, (C) CP, and (D) LP, in which the inhibition of MAC deposition upon pathway specific activation was assessed per manufacturer’s protocol. (B) Antibodies were also tested in AP hemolysis assay. Rabbit erythrocytes were incubated with 10% <t>NHS</t> containing Mg 2+ -EGTA in the presence of varying concentrations of either SAR443809 (●) or the isotype control (○). The extent of hemolysis was quantified using the absorbance of the released hemoglobin in the supernatant. The data reported here is a mean of 3 independent experiments ± standard deviation, for all 4 data sets.
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    SAR443809 specifically inhibits AP without inhibiting CP or LP. SAR443809 and its corresponding IgG isotype control were tested in a series of assays to assess their ability to inhibit the different complement pathways. A dose-dilution series of SAR443809 (●) or IgG control antibody (○) were tested in the commercial Wieslab EIA assays for (A) AP, (C) CP, and (D) LP, in which the inhibition of MAC deposition upon pathway specific activation was assessed per manufacturer’s protocol. (B) Antibodies were also tested in AP hemolysis assay. Rabbit erythrocytes were incubated with 10% NHS containing Mg 2+ -EGTA in the presence of varying concentrations of either SAR443809 (●) or the isotype control (○). The extent of hemolysis was quantified using the absorbance of the released hemoglobin in the supernatant. The data reported here is a mean of 3 independent experiments ± standard deviation, for all 4 data sets.

    Journal: Blood Advances

    Article Title: SAR443809: a selective inhibitor of the complement alternative pathway, targeting complement factor Bb

    doi: 10.1182/bloodadvances.2022009028

    Figure Lengend Snippet: SAR443809 specifically inhibits AP without inhibiting CP or LP. SAR443809 and its corresponding IgG isotype control were tested in a series of assays to assess their ability to inhibit the different complement pathways. A dose-dilution series of SAR443809 (●) or IgG control antibody (○) were tested in the commercial Wieslab EIA assays for (A) AP, (C) CP, and (D) LP, in which the inhibition of MAC deposition upon pathway specific activation was assessed per manufacturer’s protocol. (B) Antibodies were also tested in AP hemolysis assay. Rabbit erythrocytes were incubated with 10% NHS containing Mg 2+ -EGTA in the presence of varying concentrations of either SAR443809 (●) or the isotype control (○). The extent of hemolysis was quantified using the absorbance of the released hemoglobin in the supernatant. The data reported here is a mean of 3 independent experiments ± standard deviation, for all 4 data sets.

    Article Snippet: Complement preserved normal human serum (NHS) was sourced from Complement Technology Inc (Tyler, TX), BioIVT (Westbury, NY), or Innovative Research (Novi, MI).

    Techniques: Control, Inhibition, Activation Assay, Hemolysis Assay, Incubation, Standard Deviation