Journal: Cancer Biology & Medicine
Article Title: VDAC1 protein derived from extracellular vesicles promotes paclitaxel resistance in gastric cancer through autophagy and mitophagy
doi: 10.20892/j.issn.2095-3941.2025.0360
Figure Lengend Snippet: Proteomic landscapes were established in EVs derived from PTX-resistant cells. (A) Schematic diagram of the proteomics design. (B) Volcano diagram analysis of differentially expressed proteins in PTX-sensitive (AGS) and -resistant cells (AGSR) and the secreted EVs (AGS-EVs or AGSR-EVs). (C) Venn diagram analysis of the differentially expressed proteins between the cell (AGSR) and the secreted EVs (AGSR-EVs). (D) Heatmap analysis of the overlapping proteins in cells (AGSR) and EVs (AGSR-EVs). (E) KEGG analysis of overlapped proteins in cells (AGSR) and EVs (AGSR-EVs). (F) The expression trends of the top 10 overlapping proteins in cells (AGSR) and EVs (AGSR-EVs) were analyzed using scatter plots. (G) The levels of VDAC1, HTRA1, and STEAP4 expression in cells (AGSR and HGC-27R) and EVs (AGSR-EVs and HGC-27R-EVs) were detected by western blot analysis. EV, extracellular vesicle; HTRA1, HtrA serine peptidase 1; PTX, paclitaxel; STEAP4, Six Transmembrane Epithelial Antigen of Prostate 4; VDAC1, voltage-dependent anion channel protein 1.
Article Snippet: The primary antibodies used in western blots were as follows: β-actin (1:1000, T002; Affinity, Cincinnati, OH, USA); GAPDH (1:5000, 104941-AP; Proteintech, Wuhan, China); P-gp (1:3000, 22336-1-AP; Proteintech); CD9 (1:1000, sc-13118; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), HSP90 (1:5000, 13171-1-AP; Proteintech); calnexin (1:1000; c-23954; Santa Cruz Biotechnology, Inc. USA); TSG101 (1:1000, c-7964; Santa Cruz Biotechnology, Inc.); VDAC1 (1:1000, PTM-6157; PTM Biolabs, Chicago, IL, USA); p62 (1:1000, #5114; Cell Signaling Technology, Danvers, Massachusetts, USA); LC3B (1:1000, L7543; Sigma); ULK1 (1:1000, 20986-1-AP; Proteintech); p-ULK1 (1:1000, #14202; Cell Signaling Technology); AMPK (1:1000, #5831; Cell Signaling Technology); p-AMPK (1:1000, #2535; Cell Signaling Technology); mTOR (1:5000, 20657-1-AP; Proteintech); p-mTOR (1:2000, 67778-1-Ig; Proteintech); p70S6K (1:2000, 14485-1-AP; Proteintech); p-p70S6K (1:1000, #AF3228; Affinity); ATG5 (1:1000, 10181-2-AP; Proteintech); ATG7 (1:1000, 10088-2-AP; Proteintech); HTRA1 (1:1000, 55011-1-AP, Proteintech); and STEAP4 (1:1000, 11944-1-AP; Proteintech).
Techniques: Derivative Assay, Expressing, Western Blot
Proteintech is a verified supplier 
