hek bluetm cells expressing htlr7  (InvivoGen)

Journal: Molecular Neurobiology

Article Title: Microglia-Derived Extracellular Vesicles from Alzheimer’s Disease Patients Carry miRNAs Driving a Neuroinflammatory Response

doi: 10.1007/s12035-026-05719-w

Figure Lengend Snippet: AD EV-associated miRNAs activate hTLR8 . HEK293-derived reporter cells expressing hTLR7 ( a ) or hTLR8 ( b ), and their corresponding parental control lines Null1K and Null1, were stimulated with 10 μg/mL of miRNA mimics let-7e , miR-3150b-3p, and miR-548b-3p, as indicated, for 24 h. Loxoribine (1 mM) and R848 (10 μg/mL) served as positive control for TLR7 and TLR7/8 activation, respectively. TNF (0,1 μg/mL) served as positive control for HEK293-derived cell line activation. Unstimulated cells served as negative control. Results are expressed as mean ± SEM. n = 3. Significance was determined using Welch’s two-sided t -test adjusted for multiple comparisons. The vertical axis represents fold change. * p < 0.05; ** p < 0.01; *** p < 0.001

Article Snippet: HEK BlueTM cells expressing hTLR7 or hTLR8, as well as the respective control cell lines HEK-BlueTM Null1-k and Null1 (InvivoGen, San Diego, CA, USA) were cultured in DMEM (Invitrogen #41,965,062, Carlsbad, CA, USA).

Techniques: Derivative Assay, Expressing, Control, Positive Control, Activation Assay, Negative Control

Journal: medRxiv

Article Title: A highly prevalent lupus risk haplotype increases IRF7-dependent induction of IFN-α, enhancing antiviral defense and exacerbating autoimmunity

doi: 10.64898/2026.01.21.26344474

Figure Lengend Snippet: (A) Schematic of IRF7 activation, nuclear localization, and DNA binding downstream of multiple pattern recognition receptor pathways. (B) Overview of experimental approach employed in HEK Blue hTLR7 cells. C) Representative Western blot image of HEK Blue hTLR7 nuclear lysates following stimulation with R848 for 3-hours. β-actin and total H3 are included as loading controls. (D) Densitometry analysis of band intensities for risk and protective haplotype bands based on the quantitative Western blot presented in Figure S2. IRF7 bands were normalized to a total protein stain to calculate normalized signal as described in Methods. Welch’s t-test was used to determine significance. (E) Normalized ISRE luciferase activity (ratio of ISRE firefly to Renilla luciferase relative light units (RLU)) following 24-hours of R848 stimulation. Welch’s t-test was used to determine significance. (F) IFN-α measured in cell-free supernatants from cells stimulated with R848 for 3– and 24-hours. Data are mean ± SEM of triplicate samples (unless otherwise indicated) and are representative of at least three independent experiments. Significance was assessed with two-way ANOVA with a Holm-Šidak’s multiple comparisons correction. *P<0.05; ***P<0.005; ns indicates non-significant (P>0.05). ISRE: Interferon stimulated response element; EV: Empty Vector; R848: Resiquimod.

Article Snippet: THP-1 IRF7 deficient and HEK Blue hTLR7 cells were purchased from InvivoGen.

Techniques: Activation Assay, Binding Assay, Western Blot, Staining, Luciferase, Activity Assay, Plasmid Preparation

Journal: Cell

Article Title: Distinct components of mRNA vaccines cooperate to instruct efficient germinal center responses

doi: 10.1016/j.cell.2025.11.023

Figure Lengend Snippet: (A) Representative flow cytometry of Tfh cells, GC B cells, and Spike-specific GC B cells (S+) from wild type (WT) and Myd88 −/− mice. (B) Tfh cell, GC B cell, and S+ GC B cell absolute numbers, as detailed in A . (C) Absolute numbers of Tfh cells and GC B cells, as detailed in A , in WT and Tlr7 −/− mice. (D) Frequencies and absolute numbers of Tfh cells and GC B cells, as detailed in A , in mice treated with isotype, anti-IL-1R, or anti-IL-18 mAb. (E) IFN-α levels 8 hours after immunization. Mice were treated with isotype or anti-IL-1R mAb. (F) IFN-α levels in WT and Irf3/7−/− mice 8 hours after immunization. (G) Frequency and absolute numbers of Tfh cells and GC B cells, as detailed in A , in WT and Irf3/7−/− mice. In ( A-G ), mice received 3 μg of Spikevax; n = 6-10 mice per group from 2-3 independent experiments. An unpaired two-tailed Mann-Whitney U test was conducted.

Article Snippet: Human TLR7 Dual Reporter HEK 293 Cells , InvivoGen , Cat#hkd-htlr7.

Techniques: Flow Cytometry, Two Tailed Test, MANN-WHITNEY