Journal: bioRxiv
Article Title: Identification a Compact Promoter using a New Promoter Selection Strategy and Engineering Hybrid Pol II/III Enable Efficient Genome Editing in Human Retinal Ganglion Cells
doi: 10.64898/2026.01.26.701896
Figure Lengend Snippet: Correlation analysis was performed to compare exogenous transgene expressions by luciferase reporter assays with endogenous gene expression data in four cell types (RNA-seq): Hela (a cervical cancer cell line), HT1080 (a fibrosarcoma line), WI-38 (primary human fibroblasts), and mouse embryonic fibroblasts (MEFs). R 2 denotes the correlation of determination derived from the correlation coefficient(r), to indicate the strength of the linear relationship. A-D) Correlation analysis for forward promoters. E-H) Correlation analysis for forward promoters.
Article Snippet: Luciferase expression data from over 300 bidirectional promoters in four cell lines—HeLa (human cervical cancer), WI-38 (human primary fibroblasts), HT1080 (human fibrosarcoma), and mouse embryonic fibroblasts (MEFs; ATCC)—were obtained from Trinklein et al. [ ].
Techniques: Luciferase, Gene Expression, RNA Sequencing, Derivative Assay