Journal: bioRxiv
Article Title: Matrix mechanics governs mechano-metabolic adaptation across cancer grades in bladder spheroids
doi: 10.64898/2025.12.03.692076
Figure Lengend Snippet: Spheroids representing three bladder cancer cell lines (HCV29, T24, HT1376) were embedded in collagen–hyaluronan (Col–HA) hydrogels. Relative expressions of E-cadherin, N-cadherin, hexokinase 2 (HK2), and syndecan-4 (SDC4) were normalized to control (i.e., expressions in spheroids grown only in culture medium, n = 3) and converted to fold change. Statistical significance was obtained by unpaired Student’s t-test at the level of 0.05 (* p < 0.01, **p <0.001, ***p <0.0001).
Article Snippet: Roswell Park Memorial Institute 1640 culture medium (RPMI, Merck, Poland) supplemented with 10% Fetal Bovine Serum (FBS, ATCC, USA) was used for HCV29 and T24 cells, and Eagle’s Minimum Essential Medium (EMEM, ATCC, USA) with 10% FBS for HT1376 cells.
Techniques: Control