Journal: Discover Oncology
Article Title: Identification and validation of LDHA and SLC16A1 for predicting prognosis and diagnosis in lower-grade glioma
doi: 10.1007/s12672-025-03297-2
Figure Lengend Snippet: Expression of SLC16A1 in SW1088 and HS683 after transfection with si-SLC16A1. A The expressions of LDHA and SLC16A1 in low-grade glioma cell lines. The X-axis represented different cells, while the Y-axis represented the relative expression of LDHA and SLC16A1. Control, normal human glial cells; Low-grade glioma cell lines SW1088 and HS683. **, P < 0.01, when compared with control. B The mRNA expression of SLC16A1 was detected by qRT-PCR. C The protein expression of SLC16A1 was detected by western blot. Protein concentrations were determined by BCA assay, and equal amounts (30 µg) were loaded in each lane.***, P < 0.001
Article Snippet: The LGG cell lines of SW1088 and HS683 cell lines were obtained from the American Type Culture Collection (ATCC, USA).
Techniques: Expressing, Transfection, Control, Quantitative RT-PCR, Western Blot, BIA-KA