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nrg1 b1 hrg b1  (R&D Systems)


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    Structured Review

    R&D Systems nrg1 b1 hrg b1
    Nrg1 B1 Hrg B1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hrg/Recombinant+Human+NRG1-beta+1%2FHRG1-beta+1+EGF+Domain+Protein/10__1158_slash_0008___5472__can___25___4018-282-40-42
    Average 95 stars, based on 124 article reviews
    nrg1 b1 hrg b1 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Unbiased Combinatorial Screening Identifies a Bispecific IgG1 that Potently Inhibits HER3 Signaling via HER2-Guided Ligand Blockade.
    Article Snippet: .. HRG (R&D Systems, Cat396-HB) was then added to a final concentration of 12.5 nM. .. After 1, 3, 6 or 24 hr, dishes were placed on ice, washed twice with cold PBS and cells were lysed in RIPA lysis buffer (Cell Signaling Technology, Cat#9806) supplemented with Protease Inhibitor Cocktail (Cell Signaling Technology, Cat#5871).

    Incubation:

    Article Title: Origin of diverse phosphorylation patterns in the ERBB system
    Article Snippet: HeLa, A431, and MCF7 cells were cultured in DMEM (WAKO #044– 29765) containing 10% FBS to high confluence in 35-mm dishes under 5% CO2 at 37 C. One day before the experiment, the cells were starved in DMEM without fetal bovine serum and phenol red. .. The cells were stimulated with 20 nM EGF (PEPROTECH) or 30 nM HRG (R&D Systems) and incubated for 5, 10, 15, 30, and 60 min at 25 C. At each time point, the cells were washed with PBS and lysed in 100 mL of 1 SDS sample buffer containing 1 mM Na3VO4 to avoid dephosphorylation during the sample preparation. ..

    De-Phosphorylation Assay:

    Article Title: Origin of diverse phosphorylation patterns in the ERBB system
    Article Snippet: HeLa, A431, and MCF7 cells were cultured in DMEM (WAKO #044– 29765) containing 10% FBS to high confluence in 35-mm dishes under 5% CO2 at 37 C. One day before the experiment, the cells were starved in DMEM without fetal bovine serum and phenol red. .. The cells were stimulated with 20 nM EGF (PEPROTECH) or 30 nM HRG (R&D Systems) and incubated for 5, 10, 15, 30, and 60 min at 25 C. At each time point, the cells were washed with PBS and lysed in 100 mL of 1 SDS sample buffer containing 1 mM Na3VO4 to avoid dephosphorylation during the sample preparation. ..

    Sample Prep:

    Article Title: Origin of diverse phosphorylation patterns in the ERBB system
    Article Snippet: HeLa, A431, and MCF7 cells were cultured in DMEM (WAKO #044– 29765) containing 10% FBS to high confluence in 35-mm dishes under 5% CO2 at 37 C. One day before the experiment, the cells were starved in DMEM without fetal bovine serum and phenol red. .. The cells were stimulated with 20 nM EGF (PEPROTECH) or 30 nM HRG (R&D Systems) and incubated for 5, 10, 15, 30, and 60 min at 25 C. At each time point, the cells were washed with PBS and lysed in 100 mL of 1 SDS sample buffer containing 1 mM Na3VO4 to avoid dephosphorylation during the sample preparation. ..

    Cell Differentiation:

    Article Title: Metabolism-related MOGS Gene is Dysregulated After Peripheral Nerve Injury and Negatively Regulates Schwann Cell Plasticity
    Article Snippet: Cultured Schwann cells were transfected with siRNAs against MOGS (siRNA-1: RibiBio, siG2010140505022069, GUCUAUUUCGGCAUGAAGA, siRNA-2: RibiBio, siG2010140505023161, UCGGCAACAUAUCUAUGAU, and siRNA-3: RibiBio, siG2010140505024253, GUAAAGAGCCACCUAAACA) or a random sequence non-targeting negative control (RibiBio, Guangzhou, Guangdong, China) using Lipofectamine RNAiMAX transfection reagent (Invitrogen). .. Cellular differentiation was mediated by culturing cells in a differentiation medium group containing DMEM/F12 (10–092-CVR, Corning), 0.5% FBS (Gibco), 1% penicillin and streptomycin (Beyotime), 20 ng/ml HRG (R&D Systems Inc.), and 1 mM db-cAMP (Sigma). ..

    Activation Assay:

    Article Title: Differentiating cancer cells reveal early large-scale genome regulation by pericentric domains
    Article Snippet: .. For experimental studies, cells were serum starved for 48 h and treated with 50 nM HRG (396-HB (R&D Systems, Minneapolis, MN) or ab50227 (Abcam, Cambridge, UK) for different time points: 10, 15, 20, and 30 min and 1 and 3 h. For ErbB2 receptor clustering and internalization experiments proving the activation of ErbB2 receptors, after 48 h of serum starvation, additionally reduced serum starvation (0.5% FBS) for 3 h was performed. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Comparative Performance of Ante-Mortem Diagnostic Assays for the Identification of Mycobacterium bovis -Infected Domestic Dogs ( Canis lupus familiaris )
    Article Snippet: .. TNF-α was measured in stimulated (with media-only, PPDA, PPDB, or ESAT-6/CFP10 and PMA/CA as above) cell supernatants from a randomly selected subset of 45 dogs from the HRG by commercial ELISA (DY1507, R&D Systems, Europe Ltd., UK) according to the manufacturer’s instructions. ..

    Purification:

    Article Title: Metabolism-related MOGS Gene is Dysregulated After Peripheral Nerve Injury and Negatively Regulates Schwann Cell Plasticity
    Article Snippet: Cells were treated with anti-Thy1.1 (1:1000, M7898, Sigma, St. Louis, MO, USA) and rabbit complement (Invitrogen, Carlsbad, CA, USA) to remove fibroblasts. .. Purified Schwann cells were cultured in DMEM (10–013-CVR, Corning, NY, USA) containing 10% FBS (10099141c, Gibco, Grand Island, NY, USA), 1% penicillin and streptomycin (c0222, Beyotime, Shanghai, China), 2 μM forskolin (Sigma), and 10 ng/ml HRG (R&D Systems Inc., Minneapolis, MN, USA). .. Cultured Schwann cells were transfected with siRNAs against MOGS (siRNA-1: RibiBio, siG2010140505022069, GUCUAUUUCGGCAUGAAGA, siRNA-2: RibiBio, siG2010140505023161, UCGGCAACAUAUCUAUGAU, and siRNA-3: RibiBio, siG2010140505024253, GUAAAGAGCCACCUAAACA) or a random sequence non-targeting negative control (RibiBio, Guangzhou, Guangdong, China) using Lipofectamine RNAiMAX transfection reagent (Invitrogen).

    Cell Culture:

    Article Title: Metabolism-related MOGS Gene is Dysregulated After Peripheral Nerve Injury and Negatively Regulates Schwann Cell Plasticity
    Article Snippet: Cells were treated with anti-Thy1.1 (1:1000, M7898, Sigma, St. Louis, MO, USA) and rabbit complement (Invitrogen, Carlsbad, CA, USA) to remove fibroblasts. .. Purified Schwann cells were cultured in DMEM (10–013-CVR, Corning, NY, USA) containing 10% FBS (10099141c, Gibco, Grand Island, NY, USA), 1% penicillin and streptomycin (c0222, Beyotime, Shanghai, China), 2 μM forskolin (Sigma), and 10 ng/ml HRG (R&D Systems Inc., Minneapolis, MN, USA). .. Cultured Schwann cells were transfected with siRNAs against MOGS (siRNA-1: RibiBio, siG2010140505022069, GUCUAUUUCGGCAUGAAGA, siRNA-2: RibiBio, siG2010140505023161, UCGGCAACAUAUCUAUGAU, and siRNA-3: RibiBio, siG2010140505024253, GUAAAGAGCCACCUAAACA) or a random sequence non-targeting negative control (RibiBio, Guangzhou, Guangdong, China) using Lipofectamine RNAiMAX transfection reagent (Invitrogen).



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    Image Search Results


    A9B5-Bs-5 and A9B5-Bs-7 inhibit the growth of trastuzumab-resistant HER2-positive cancer cells. (A, B) Growth inhibition in NCI-N87 (A) and BT474 cells (B) treated with bpAbs in the absence of ligands, measured by CCK-8 assay. (C, D) Growth inhibition in NCI-N87 (C) and BT474 cells (D) treated with bpAbs in the presence of EGF or HRG, measured by CCK-8 assay. Serum-starved cells were incubated with anti-HER2 diluted antibodies in the presence of 1 nM HRG or 5 nM EGF. Horizontal dotted line (black) represents viability of non-treated cells referenced to 100%. Data represent mean ± SD (n = 3). (E) Heat map of EC 50 values (nM) derived from CCK-8 assays showing dose-dependent inhibition by bpAbs. Source data are available in the Source Data file.

    Journal: Frontiers in Immunology

    Article Title: Engineering HER2-targeted biparatopic antibodies to promote receptor internalization and restore antitumor efficacy

    doi: 10.3389/fimmu.2025.1711433

    Figure Lengend Snippet: A9B5-Bs-5 and A9B5-Bs-7 inhibit the growth of trastuzumab-resistant HER2-positive cancer cells. (A, B) Growth inhibition in NCI-N87 (A) and BT474 cells (B) treated with bpAbs in the absence of ligands, measured by CCK-8 assay. (C, D) Growth inhibition in NCI-N87 (C) and BT474 cells (D) treated with bpAbs in the presence of EGF or HRG, measured by CCK-8 assay. Serum-starved cells were incubated with anti-HER2 diluted antibodies in the presence of 1 nM HRG or 5 nM EGF. Horizontal dotted line (black) represents viability of non-treated cells referenced to 100%. Data represent mean ± SD (n = 3). (E) Heat map of EC 50 values (nM) derived from CCK-8 assays showing dose-dependent inhibition by bpAbs. Source data are available in the Source Data file.

    Article Snippet: For ligand-dependent assays, tumor cells were stimulated with either 1 nM HRG (SinoBiological, 11609-HNCH) or 5 nM EGF (SinoBiological, GMP-10605-HNAE).

    Techniques: Inhibition, CCK-8 Assay, Incubation, Derivative Assay