Journal: Nature Communications

Article Title: Mapping of mitogen and metabolic sensitivity in organoids defines requirements for human hepatocyte growth

doi: 10.1038/s41467-024-48550-4

Figure Lengend Snippet: a Heatmap displaying gene expression patterns of cytokines, chemokines, and growth factors upon FH organoid growth from tissue. The mean expression of n = 2 donors is visualized as row Z -scores. b Comparison between the temporal mRNA expression profiles of selected cytokines and growth factors upon hepatocyte organoid growth from tissue (black) or single FHs (green). The mean expression of n = 2 donors per dataset is plotted (normalized transcripts). c Experimental strategy to evaluate the effect of cytokines and growth factors on human FH organoid growth. d Representative brightfield images of organoid-derived single FHs at day 0 and the outgrowing organoids challenged with indicated cytokines and growth factors at day 7. Representative of n = 2 challenge experiments. Scale bar = 100 μm. e Quantification of the organoid diameter 7 days post organoid outgrowth from single FHs under the different challenges ( n = 40 organoids per condition). Mean ± SD is plotted. EREG: * p = 0.0143; AREG: *** p = 0.0005; NRG1, IL1β, IL6, IL10, IL11: *** p < 0.0001, all versus control, one-way ANOVA with Dunnett’s post hoc test. Source data are provided as a Source data file.

Article Snippet: To optimize PHH organoid growth, different conditions were tested, including supplementation of the FH expansion medium with combinations of 100 ng/ml IL6, 20 ng/ml IL11, 20 ng/ml IL1β, or 100 ng/ml NRG1, and 10 μM cilofexor (GS-9674, FXRa) (MedChemExpress, HY-109083).

Techniques: Gene Expression, Expressing, Comparison, Derivative Assay, Control

Journal: Oncotarget

Article Title: The anti-HER3 antibody patritumab abrogates cetuximab resistance mediated by heregulin in colorectal cancer cells

doi:

Figure Lengend Snippet: (A) DiFi isogenic cell lines (DiFi, DiFi-Mock1, DiFi-HRG4, DiFi-HRG5, and DiFi-HRG6) were cultured overnight in medium containing 10% serum and then incubated for 24 h in serum-free medium, after which the cells were lysed and subjected to immunoblot analysis with antibodies to heregulin and to β-actin (loading control). (B) Culture supernatants from cells cultured as described in Materials and Methods were assayed for heregulin with an ELISA. Data are means ± SE from three independent experiments. * P < 0.05 (Student's t test) for comparison of each DiFi-HRG line with DiFi-Mock1 or DiFi cells. (C) Cells were treated with cetuximab at the indicated concentrations for 5 days, after which cell viability was assessed. Data are means ± SE from three independent experiments.

Article Snippet: A full-length cDNA encoding human heregulin (NRG1, GenBank accession no. NM_013956) was obtained from Origene (Rockville, MD).

Techniques: Cell Culture, Incubation, Western Blot, Control, Enzyme-linked Immunosorbent Assay, Comparison

Journal: Oncotarget

Article Title: The anti-HER3 antibody patritumab abrogates cetuximab resistance mediated by heregulin in colorectal cancer cells

doi:

Figure Lengend Snippet: (A) DiFi colorectal cancer cells treated with cetuximab alone. (B) DiFi cells that stably overexpress heregulin (DiFi-HRG cells) are resistant to cetuximab as a result of HER2-HER3 heterodimerization and AKT activation induced by heregulin. (C) Patritumab abrogates cetuximab resistance mediated by the heregulin autocrine loop in DiFi-HRG cells.

Article Snippet: A full-length cDNA encoding human heregulin (NRG1, GenBank accession no. NM_013956) was obtained from Origene (Rockville, MD).

Techniques: Stable Transfection, Activation Assay

Journal: EMBO Molecular Medicine

Article Title: Histidine-rich glycoprotein modulates neutrophils and thrombolysis-associated hemorrhagic transformation

doi: 10.1038/s44321-024-00117-y

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Rabbit anti-HRG , Proteintech , 26252-1-AP.

Techniques: Clinical Proteomics, Recombinant, Sequencing, In Vivo, SYBR Green Assay, Software, Bradford Assay, Enzyme-linked Immunosorbent Assay, Membrane