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hoxd9  (Proteintech)


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    Structured Review

    Proteintech hoxd9
    Hoxd9, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hoxd9/HOXD9+Antibody/10__1172_slash_jci181243-246-38-40
    Average 93 stars, based on 3 article reviews
    hoxd9 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Western Blot:

    Article Title: Gut microbial metabolite 4-hydroxybenzeneacetic acid drives colorectal cancer progression via accumulation of immunosuppressive PMN-MDSCs
    Article Snippet: Main Chemical reagents used in WB 587 Stattic(Sellect, Cat #S7024, 20 μM) , Colivelin (Sellect, Cat #S9664, 20 μM), ITSA-1 588 (Sellect, Cat #S8323, 50 μM), Panobinostat (Sellect, Cat #S1030, 50 nM). .. 589 Immunoblot analysis of cell lysates (20-60 mg) in RIPA buffer was carried out to 590 assess protein expression in the presence of rabbit antibodies against IRF1 (Rabbit, 591 Proteintech, Cat#11335-1-AP, 1:1000), OCT1 (Rabbit, Proteintech, Cat#10387-1-AP, 592 1:1000), HOXD9 (Rabbit, Proteintech, Cat# 20560-1-AP, 1:1000), HOXD10 (Rabbit, 593 abcam, Cat#ab138508, 1:1000), p-STAT3 (Rabbit, CST, Cat#9145, 1:1000), STAT3 594 (Mouse, CST, Cat#9139, 1:1000), JAK2 (Rabbit, CST, Cat#3230, 1:1000), p-JAK2 595 (Rabbit, CST, Cat#3771, 1:1000), CXCL3 (Rabbit, ThermoFisher, Cat#PA5-103136), 596 GAPDH (Mouse, ZSbio, Cat#TA-08, 1:1000). ..

    Article Title: Gut microbial metabolite 4-hydroxybenzeneacetic acid drives colorectal cancer progression via accumulation of immunosuppressive PMN-MDSCs
    Article Snippet: The main chemical reagents used in WB were Stattic (Selleckchem, S7024; 20 μM), Colivelin (Selleckchem, S9664; 20 μM), ITSA-1 (Selleckchem, S8323; 50 μM), and Panobinostat (Selleckchem, S1030; 50 nM). .. Immunoblot analysis of cell lysates (20–60 mg) in RIPA buffer was carried out to assess protein expression in the presence of rabbit antibodies against IRF1 (rabbit; Proteintech, 11335-1-AP; 1:1,000), OCT1 (rabbit; Proteintech, 10387-1-AP; 1:1,000), HOXD9 (rabbit; Proteintech, 20560-1-AP; 1:1,000), HOXD10 (rabbit; Abcam, ab138508; 1:1,000), p-STAT3 (rabbit; CST, 9145; 1:1,000), STAT3 (mouse; CST, 9139; 1:1,000), JAK2 (rabbit; CST, 3230; 1:1,000), p-JAK2 (rabbit; CST, 3771; 1:1,000), CXCL3 (rabbit; Thermo Fisher Scientific, PA5-103136; 1:1,000), and GAPDH (mouse; ZSbio, TA-08; 1:1,000). ..

    Expressing:

    Article Title: Gut microbial metabolite 4-hydroxybenzeneacetic acid drives colorectal cancer progression via accumulation of immunosuppressive PMN-MDSCs
    Article Snippet: Main Chemical reagents used in WB 587 Stattic(Sellect, Cat #S7024, 20 μM) , Colivelin (Sellect, Cat #S9664, 20 μM), ITSA-1 588 (Sellect, Cat #S8323, 50 μM), Panobinostat (Sellect, Cat #S1030, 50 nM). .. 589 Immunoblot analysis of cell lysates (20-60 mg) in RIPA buffer was carried out to 590 assess protein expression in the presence of rabbit antibodies against IRF1 (Rabbit, 591 Proteintech, Cat#11335-1-AP, 1:1000), OCT1 (Rabbit, Proteintech, Cat#10387-1-AP, 592 1:1000), HOXD9 (Rabbit, Proteintech, Cat# 20560-1-AP, 1:1000), HOXD10 (Rabbit, 593 abcam, Cat#ab138508, 1:1000), p-STAT3 (Rabbit, CST, Cat#9145, 1:1000), STAT3 594 (Mouse, CST, Cat#9139, 1:1000), JAK2 (Rabbit, CST, Cat#3230, 1:1000), p-JAK2 595 (Rabbit, CST, Cat#3771, 1:1000), CXCL3 (Rabbit, ThermoFisher, Cat#PA5-103136), 596 GAPDH (Mouse, ZSbio, Cat#TA-08, 1:1000). ..

    Article Title: Gut microbial metabolite 4-hydroxybenzeneacetic acid drives colorectal cancer progression via accumulation of immunosuppressive PMN-MDSCs
    Article Snippet: The main chemical reagents used in WB were Stattic (Selleckchem, S7024; 20 μM), Colivelin (Selleckchem, S9664; 20 μM), ITSA-1 (Selleckchem, S8323; 50 μM), and Panobinostat (Selleckchem, S1030; 50 nM). .. Immunoblot analysis of cell lysates (20–60 mg) in RIPA buffer was carried out to assess protein expression in the presence of rabbit antibodies against IRF1 (rabbit; Proteintech, 11335-1-AP; 1:1,000), OCT1 (rabbit; Proteintech, 10387-1-AP; 1:1,000), HOXD9 (rabbit; Proteintech, 20560-1-AP; 1:1,000), HOXD10 (rabbit; Abcam, ab138508; 1:1,000), p-STAT3 (rabbit; CST, 9145; 1:1,000), STAT3 (mouse; CST, 9139; 1:1,000), JAK2 (rabbit; CST, 3230; 1:1,000), p-JAK2 (rabbit; CST, 3771; 1:1,000), CXCL3 (rabbit; Thermo Fisher Scientific, PA5-103136; 1:1,000), and GAPDH (mouse; ZSbio, TA-08; 1:1,000). ..



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    <t>HOXD9</t> expression in U87 cells transfected with the vector or HOXD9 overexpression. A The mRNA was quantified. B The protein was quantified. ** p < 0.01, *** p < 0.001. C qRT-PCR detected the mRNA expression of PLEKHA4 , *** p < 0.001. D Western Blot detected the protein expression of PLEKHA4 , *** p < 0.001. E Luciferase reporter assay shows that HOXD9 binds to PLEKHA4 , *** p < 0.001. The luciferase reporter plasmid containing WT or MUT- PLEKHA4 was co-transfected into U87 cells with HOXD9 overexpression or vector. F The mRNA, and G , H . The protein expression levels of HOXD9 and PLEKHA4 in U87 cells co-transfected with sh PLEKHA4 and HOXD9 overexpression. *** p < 0.001 compared with Vector + shNC, # p < 0.05, ### p < 0.001 compared with Vector + sh PLEKHA4 group. I CCK-8 detected the activity of the U87 cells. *** p < 0.001 compared with Vector + shNC group, ### p < 0.001 compared with Vector + sh PLEKHA4 group. J , K Flow cytometry detected apoptosis. *** p < 0.001 compared with Vector + shNC group, ### p < 0.001 compared with Vector + sh PLEKHA4 group. L Glucose u p take assays, M lactate production, N ATP production were measured, *** p < 0.001 compared with Vector + shNC group, ## p < 0.01, ### p < 0.001 compared with Vector + sh PLEKHA4 group. The cell experiments were conducted three independent cultures, with two replicates conducted each time.
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    <t>HOXD9</t> expression in U87 cells transfected with the vector or HOXD9 overexpression. A The mRNA was quantified. B The protein was quantified. ** p < 0.01, *** p < 0.001. C qRT-PCR detected the mRNA expression of PLEKHA4 , *** p < 0.001. D Western Blot detected the protein expression of PLEKHA4 , *** p < 0.001. E Luciferase reporter assay shows that HOXD9 binds to PLEKHA4 , *** p < 0.001. The luciferase reporter plasmid containing WT or MUT- PLEKHA4 was co-transfected into U87 cells with HOXD9 overexpression or vector. F The mRNA, and G , H . The protein expression levels of HOXD9 and PLEKHA4 in U87 cells co-transfected with sh PLEKHA4 and HOXD9 overexpression. *** p < 0.001 compared with Vector + shNC, # p < 0.05, ### p < 0.001 compared with Vector + sh PLEKHA4 group. I CCK-8 detected the activity of the U87 cells. *** p < 0.001 compared with Vector + shNC group, ### p < 0.001 compared with Vector + sh PLEKHA4 group. J , K Flow cytometry detected apoptosis. *** p < 0.001 compared with Vector + shNC group, ### p < 0.001 compared with Vector + sh PLEKHA4 group. L Glucose u p take assays, M lactate production, N ATP production were measured, *** p < 0.001 compared with Vector + shNC group, ## p < 0.01, ### p < 0.001 compared with Vector + sh PLEKHA4 group. The cell experiments were conducted three independent cultures, with two replicates conducted each time.
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    Image Search Results


    HOXD9 expression in U87 cells transfected with the vector or HOXD9 overexpression. A The mRNA was quantified. B The protein was quantified. ** p < 0.01, *** p < 0.001. C qRT-PCR detected the mRNA expression of PLEKHA4 , *** p < 0.001. D Western Blot detected the protein expression of PLEKHA4 , *** p < 0.001. E Luciferase reporter assay shows that HOXD9 binds to PLEKHA4 , *** p < 0.001. The luciferase reporter plasmid containing WT or MUT- PLEKHA4 was co-transfected into U87 cells with HOXD9 overexpression or vector. F The mRNA, and G , H . The protein expression levels of HOXD9 and PLEKHA4 in U87 cells co-transfected with sh PLEKHA4 and HOXD9 overexpression. *** p < 0.001 compared with Vector + shNC, # p < 0.05, ### p < 0.001 compared with Vector + sh PLEKHA4 group. I CCK-8 detected the activity of the U87 cells. *** p < 0.001 compared with Vector + shNC group, ### p < 0.001 compared with Vector + sh PLEKHA4 group. J , K Flow cytometry detected apoptosis. *** p < 0.001 compared with Vector + shNC group, ### p < 0.001 compared with Vector + sh PLEKHA4 group. L Glucose u p take assays, M lactate production, N ATP production were measured, *** p < 0.001 compared with Vector + shNC group, ## p < 0.01, ### p < 0.001 compared with Vector + sh PLEKHA4 group. The cell experiments were conducted three independent cultures, with two replicates conducted each time.

    Journal: Oncogenesis

    Article Title: PLEKHA4 is transcriptionally regulated by HOXD9 and regulates glycolytic reprogramming and progression in glioblastoma via activation of the STAT3/SOCS-1 pathway

    doi: 10.1038/s41389-025-00559-0

    Figure Lengend Snippet: HOXD9 expression in U87 cells transfected with the vector or HOXD9 overexpression. A The mRNA was quantified. B The protein was quantified. ** p < 0.01, *** p < 0.001. C qRT-PCR detected the mRNA expression of PLEKHA4 , *** p < 0.001. D Western Blot detected the protein expression of PLEKHA4 , *** p < 0.001. E Luciferase reporter assay shows that HOXD9 binds to PLEKHA4 , *** p < 0.001. The luciferase reporter plasmid containing WT or MUT- PLEKHA4 was co-transfected into U87 cells with HOXD9 overexpression or vector. F The mRNA, and G , H . The protein expression levels of HOXD9 and PLEKHA4 in U87 cells co-transfected with sh PLEKHA4 and HOXD9 overexpression. *** p < 0.001 compared with Vector + shNC, # p < 0.05, ### p < 0.001 compared with Vector + sh PLEKHA4 group. I CCK-8 detected the activity of the U87 cells. *** p < 0.001 compared with Vector + shNC group, ### p < 0.001 compared with Vector + sh PLEKHA4 group. J , K Flow cytometry detected apoptosis. *** p < 0.001 compared with Vector + shNC group, ### p < 0.001 compared with Vector + sh PLEKHA4 group. L Glucose u p take assays, M lactate production, N ATP production were measured, *** p < 0.001 compared with Vector + shNC group, ## p < 0.01, ### p < 0.001 compared with Vector + sh PLEKHA4 group. The cell experiments were conducted three independent cultures, with two replicates conducted each time.

    Article Snippet: The membranes were removed and placed in 5% nonfat milk for blocking, followed by incubation with primary antibodies overnight at dilutions of 1:5000 (anti- PLEKHA4 ; Abcam, Ab84727, UK), 1:2000 (anti- HOXD9 ; CST, 55962, USA), 1:2000 (anti- STAT3 ; Abcam, Ab119352 , UK), 1:3000 (anti- p-STAT3 ; Abcam, Ab76315, UK), 1:1000 (anti- SOCS1 ; Abcam, Ab280886 , UK), and 1:5000 (anti- β-actin ; Proteintech, 66009-1-Ig, Wuhan China).

    Techniques: Expressing, Transfection, Plasmid Preparation, Over Expression, Quantitative RT-PCR, Western Blot, Luciferase, Reporter Assay, CCK-8 Assay, Activity Assay, Flow Cytometry