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hoechst33342  (Beyotime)


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    Structured Review

    Beyotime hoechst33342
    Hoechst33342, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 7099 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hoechst33342/Hoechst+33342/pmc12830281-86-25-27
    Average 99 stars, based on 7099 article reviews
    hoechst33342 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Live-cell decoding of labile post-translational modifications in APE1 with a rationally engineered nano-catcher
    Article Snippet: The cells were then incubated overnight at 4°C with 100 μl APE1 mouse monoclonal antibodies (Abcam, Cambridge, UK) diluted 1:100 in 5% (w/v) BSA. .. After being washed by PBS for five times, the cells were incubated in 100 μl Alexa Fluor 647 goat anti-mouse (Abcam, Cambridge, UK), diluted 1:500 in 5% (w/v) BSA for 1 h. Nuclei were counterstained with Hoechst33342 (Beyotime, Shanghai, China). .. For the double immunofluorescence of APE1 and NPM1, sequential incubations were carried out to bind APE1 and NPM1 to their primary antibodies and visualized by FITC- and Alexa Fluor 647-labeled secondary antibodies (Abcam, Cambridge, UK), respectively.

    Article Title: A pH-responsive dual-drug nanoplatform for stromal remodeling and enhanced chemotherapy via MMP3/TGF- β inhibition
    Article Snippet: .. After 24 h, the nanosystem was added to each well at 1 μg/mL DiD and incubated for 4 h. Then, the cells were stained with Hoechst33342 (Blue, Beyotime) for visualization under an inverted fluorescence microscope. .. Meanwhile, the cellular uptake of the nanosystem was also quantified by flow cytometry (CytoFlex, Beckman, USA).

    Article Title: The MIF-CD74 axis drives colorectal cancer via glycolytic reprogramming and is targeted by a novel small-molecule inhibitor.
    Article Snippet: .. Nuclei stained with Hoechst33342 (Beyotime), incubated at room temperature protected from light for 10 min. Hoechst33342 solution aspirated, washed twice with PBS. ..

    Article Title: TKT inhibition attenuates cardiac fibrosis in myocardial infarction through deactivating AKT signaling pathway
    Article Snippet: .. NRCFs were incubated with 50 mM EDU (C0078S, Beyotime, China) for 2 h. Nuclei were stained with Hoechst33342 for 30 min. ..

    Article Title: TKT inhibition attenuates cardiac fibrosis in myocardial infarction through deactivating AKT signaling pathway.
    Article Snippet: .. NRCFs were incubated with 50 mM EDU (C0078S, Beyotime, China) for 2 h. Nuclei were stained with Hoechst33342 for 30 min. ..

    Staining:

    Article Title: A pH-responsive dual-drug nanoplatform for stromal remodeling and enhanced chemotherapy via MMP3/TGF- β inhibition
    Article Snippet: .. After 24 h, the nanosystem was added to each well at 1 μg/mL DiD and incubated for 4 h. Then, the cells were stained with Hoechst33342 (Blue, Beyotime) for visualization under an inverted fluorescence microscope. .. Meanwhile, the cellular uptake of the nanosystem was also quantified by flow cytometry (CytoFlex, Beckman, USA).

    Article Title: The MIF-CD74 axis drives colorectal cancer via glycolytic reprogramming and is targeted by a novel small-molecule inhibitor.
    Article Snippet: .. Nuclei stained with Hoechst33342 (Beyotime), incubated at room temperature protected from light for 10 min. Hoechst33342 solution aspirated, washed twice with PBS. ..

    Article Title: GIPC1 governed ferroptosis by regulating DECR1-modulating lipid homeostasis during dilated cardiomyopathy (DCM).
    Article Snippet: .. Cells were washed with PBS and stained with Hoechst33342 (C1022, Beyotime Biotechnology, China) for 10min. .. Images were captured by Leica inverted microscopy and reading the fluorescence at to separate wavelengths; one at excitation/emission of 581/591 nm for the reduced dye, and the other at excitation/emission of 488/510 nm for the oxidized dye.

    Article Title: TKT inhibition attenuates cardiac fibrosis in myocardial infarction through deactivating AKT signaling pathway
    Article Snippet: .. NRCFs were incubated with 50 mM EDU (C0078S, Beyotime, China) for 2 h. Nuclei were stained with Hoechst33342 for 30 min. ..

    Article Title: TKT inhibition attenuates cardiac fibrosis in myocardial infarction through deactivating AKT signaling pathway.
    Article Snippet: .. NRCFs were incubated with 50 mM EDU (C0078S, Beyotime, China) for 2 h. Nuclei were stained with Hoechst33342 for 30 min. ..

    Fluorescence:

    Article Title: A pH-responsive dual-drug nanoplatform for stromal remodeling and enhanced chemotherapy via MMP3/TGF- β inhibition
    Article Snippet: .. After 24 h, the nanosystem was added to each well at 1 μg/mL DiD and incubated for 4 h. Then, the cells were stained with Hoechst33342 (Blue, Beyotime) for visualization under an inverted fluorescence microscope. .. Meanwhile, the cellular uptake of the nanosystem was also quantified by flow cytometry (CytoFlex, Beckman, USA).

    Article Title: PGLYRP4 sensitizes oral squamous cell carcinoma to ferroptosis by targeting GPX4: A novel molecular mechanism for overcoming therapeutic resistance
    Article Snippet: Cells were treated, washed with HBSS (BL561A, Biosharp, Hefei, China) twice, then stained with 1 μM FerroOrange (F374, DOJINDO, Kumamoto, Japan) at 37°C for 30 minutes. .. Afterwards, nuclei were counterstained with Hoechst33342 (C1028, Beyotime, Shanghai, China), then, images were captured by a fluorescence microscope (Nikon, Tokyo, Japan). .. ROS levels were assessed with 10 μM DCFH-DA (S0033S, Beyotime, Shanghai, China) as instructed, followed by flow cytometric analysis.

    Microscopy:

    Article Title: A pH-responsive dual-drug nanoplatform for stromal remodeling and enhanced chemotherapy via MMP3/TGF- β inhibition
    Article Snippet: .. After 24 h, the nanosystem was added to each well at 1 μg/mL DiD and incubated for 4 h. Then, the cells were stained with Hoechst33342 (Blue, Beyotime) for visualization under an inverted fluorescence microscope. .. Meanwhile, the cellular uptake of the nanosystem was also quantified by flow cytometry (CytoFlex, Beckman, USA).

    Article Title: PGLYRP4 sensitizes oral squamous cell carcinoma to ferroptosis by targeting GPX4: A novel molecular mechanism for overcoming therapeutic resistance
    Article Snippet: Cells were treated, washed with HBSS (BL561A, Biosharp, Hefei, China) twice, then stained with 1 μM FerroOrange (F374, DOJINDO, Kumamoto, Japan) at 37°C for 30 minutes. .. Afterwards, nuclei were counterstained with Hoechst33342 (C1028, Beyotime, Shanghai, China), then, images were captured by a fluorescence microscope (Nikon, Tokyo, Japan). .. ROS levels were assessed with 10 μM DCFH-DA (S0033S, Beyotime, Shanghai, China) as instructed, followed by flow cytometric analysis.



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