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anti hnrnpf  (Proteintech)


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    Proteintech anti hnrnpf
    Anti Hnrnpf, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hnrnpf/pmc12765101-53-13-15?v=Proteintech
    Average 94 stars, based on 7 article reviews
    anti hnrnpf - by Bioz Stars, 2026-08
    94/100 stars

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    <t>Hnrnpf</t> KO compromises ESC proliferation. ( A ) Schematic illustration of the experimental design to knock out Hnrnpf . Yellow lines show the recognition sites <t>of</t> <t>sgRNAs.</t> Red arrows are PCR primers for genotyping.Validation of two Hnrnpf KO ESC clones by genotyping PCR ( B ) and western blot ( C ). ( D ) Reduced proliferation rate of Hnrnpf KO ESCs. ( E ) Cell cycle analysis of E14 and Hnrnpf KO ESCs. ( F ) Colony formation assay of E14 and Hnrnpf KO ESCs. Representative phase-contrast and AP staining images are shown. Scale bar: 500 µm. The colony number ( G ) and diameter ( H ) of E14 and Hnrnpf KO ESCs are shown in panel (F). Data are presented as average ± SD ( n = 3 biological replicates). Statistical analysis was performed with student’s t -test. *, P < .05; **, P < .01; ***, P < .001.
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    <t>Hnrnpf</t> KO compromises ESC proliferation. ( A ) Schematic illustration of the experimental design to knock out Hnrnpf . Yellow lines show the recognition sites <t>of</t> <t>sgRNAs.</t> Red arrows are PCR primers for genotyping.Validation of two Hnrnpf KO ESC clones by genotyping PCR ( B ) and western blot ( C ). ( D ) Reduced proliferation rate of Hnrnpf KO ESCs. ( E ) Cell cycle analysis of E14 and Hnrnpf KO ESCs. ( F ) Colony formation assay of E14 and Hnrnpf KO ESCs. Representative phase-contrast and AP staining images are shown. Scale bar: 500 µm. The colony number ( G ) and diameter ( H ) of E14 and Hnrnpf KO ESCs are shown in panel (F). Data are presented as average ± SD ( n = 3 biological replicates). Statistical analysis was performed with student’s t -test. *, P < .05; **, P < .01; ***, P < .001.
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    <t>Hnrnpf</t> KO compromises ESC proliferation. ( A ) Schematic illustration of the experimental design to knock out Hnrnpf . Yellow lines show the recognition sites <t>of</t> <t>sgRNAs.</t> Red arrows are PCR primers for genotyping.Validation of two Hnrnpf KO ESC clones by genotyping PCR ( B ) and western blot ( C ). ( D ) Reduced proliferation rate of Hnrnpf KO ESCs. ( E ) Cell cycle analysis of E14 and Hnrnpf KO ESCs. ( F ) Colony formation assay of E14 and Hnrnpf KO ESCs. Representative phase-contrast and AP staining images are shown. Scale bar: 500 µm. The colony number ( G ) and diameter ( H ) of E14 and Hnrnpf KO ESCs are shown in panel (F). Data are presented as average ± SD ( n = 3 biological replicates). Statistical analysis was performed with student’s t -test. *, P < .05; **, P < .01; ***, P < .001.
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    <t>Hnrnpf</t> KO compromises ESC proliferation. ( A ) Schematic illustration of the experimental design to knock out Hnrnpf . Yellow lines show the recognition sites <t>of</t> <t>sgRNAs.</t> Red arrows are PCR primers for genotyping.Validation of two Hnrnpf KO ESC clones by genotyping PCR ( B ) and western blot ( C ). ( D ) Reduced proliferation rate of Hnrnpf KO ESCs. ( E ) Cell cycle analysis of E14 and Hnrnpf KO ESCs. ( F ) Colony formation assay of E14 and Hnrnpf KO ESCs. Representative phase-contrast and AP staining images are shown. Scale bar: 500 µm. The colony number ( G ) and diameter ( H ) of E14 and Hnrnpf KO ESCs are shown in panel (F). Data are presented as average ± SD ( n = 3 biological replicates). Statistical analysis was performed with student’s t -test. *, P < .05; **, P < .01; ***, P < .001.
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    <t>Hnrnpf</t> KO compromises ESC proliferation. ( A ) Schematic illustration of the experimental design to knock out Hnrnpf . Yellow lines show the recognition sites <t>of</t> <t>sgRNAs.</t> Red arrows are PCR primers for genotyping.Validation of two Hnrnpf KO ESC clones by genotyping PCR ( B ) and western blot ( C ). ( D ) Reduced proliferation rate of Hnrnpf KO ESCs. ( E ) Cell cycle analysis of E14 and Hnrnpf KO ESCs. ( F ) Colony formation assay of E14 and Hnrnpf KO ESCs. Representative phase-contrast and AP staining images are shown. Scale bar: 500 µm. The colony number ( G ) and diameter ( H ) of E14 and Hnrnpf KO ESCs are shown in panel (F). Data are presented as average ± SD ( n = 3 biological replicates). Statistical analysis was performed with student’s t -test. *, P < .05; **, P < .01; ***, P < .001.
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    <t>Hnrnpf</t> KO compromises ESC proliferation. ( A ) Schematic illustration of the experimental design to knock out Hnrnpf . Yellow lines show the recognition sites <t>of</t> <t>sgRNAs.</t> Red arrows are PCR primers for genotyping.Validation of two Hnrnpf KO ESC clones by genotyping PCR ( B ) and western blot ( C ). ( D ) Reduced proliferation rate of Hnrnpf KO ESCs. ( E ) Cell cycle analysis of E14 and Hnrnpf KO ESCs. ( F ) Colony formation assay of E14 and Hnrnpf KO ESCs. Representative phase-contrast and AP staining images are shown. Scale bar: 500 µm. The colony number ( G ) and diameter ( H ) of E14 and Hnrnpf KO ESCs are shown in panel (F). Data are presented as average ± SD ( n = 3 biological replicates). Statistical analysis was performed with student’s t -test. *, P < .05; **, P < .01; ***, P < .001.
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    <t>Hnrnpf</t> KO compromises ESC proliferation. ( A ) Schematic illustration of the experimental design to knock out Hnrnpf . Yellow lines show the recognition sites <t>of</t> <t>sgRNAs.</t> Red arrows are PCR primers for genotyping.Validation of two Hnrnpf KO ESC clones by genotyping PCR ( B ) and western blot ( C ). ( D ) Reduced proliferation rate of Hnrnpf KO ESCs. ( E ) Cell cycle analysis of E14 and Hnrnpf KO ESCs. ( F ) Colony formation assay of E14 and Hnrnpf KO ESCs. Representative phase-contrast and AP staining images are shown. Scale bar: 500 µm. The colony number ( G ) and diameter ( H ) of E14 and Hnrnpf KO ESCs are shown in panel (F). Data are presented as average ± SD ( n = 3 biological replicates). Statistical analysis was performed with student’s t -test. *, P < .05; **, P < .01; ***, P < .001.
    Mouse Anti Hnrnpf, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Hnrnpf KO compromises ESC proliferation. ( A ) Schematic illustration of the experimental design to knock out Hnrnpf . Yellow lines show the recognition sites of sgRNAs. Red arrows are PCR primers for genotyping.Validation of two Hnrnpf KO ESC clones by genotyping PCR ( B ) and western blot ( C ). ( D ) Reduced proliferation rate of Hnrnpf KO ESCs. ( E ) Cell cycle analysis of E14 and Hnrnpf KO ESCs. ( F ) Colony formation assay of E14 and Hnrnpf KO ESCs. Representative phase-contrast and AP staining images are shown. Scale bar: 500 µm. The colony number ( G ) and diameter ( H ) of E14 and Hnrnpf KO ESCs are shown in panel (F). Data are presented as average ± SD ( n = 3 biological replicates). Statistical analysis was performed with student’s t -test. *, P < .05; **, P < .01; ***, P < .001.

    Journal: Nucleic Acids Research

    Article Title: ERK phosphorylates HNRNPF to promote the proliferation and suppress the differentiation of embryonic stem cells

    doi: 10.1093/nar/gkaf1490

    Figure Lengend Snippet: Hnrnpf KO compromises ESC proliferation. ( A ) Schematic illustration of the experimental design to knock out Hnrnpf . Yellow lines show the recognition sites of sgRNAs. Red arrows are PCR primers for genotyping.Validation of two Hnrnpf KO ESC clones by genotyping PCR ( B ) and western blot ( C ). ( D ) Reduced proliferation rate of Hnrnpf KO ESCs. ( E ) Cell cycle analysis of E14 and Hnrnpf KO ESCs. ( F ) Colony formation assay of E14 and Hnrnpf KO ESCs. Representative phase-contrast and AP staining images are shown. Scale bar: 500 µm. The colony number ( G ) and diameter ( H ) of E14 and Hnrnpf KO ESCs are shown in panel (F). Data are presented as average ± SD ( n = 3 biological replicates). Statistical analysis was performed with student’s t -test. *, P < .05; **, P < .01; ***, P < .001.

    Article Snippet: Single guide RNAs (sgRNAs) targeting Hnrnpf were designed using the website https://crispor.gi.ucsc.edu/ and synthesized by the GENEWIZ Company.

    Techniques: Knock-Out, Biomarker Discovery, Clone Assay, Western Blot, Cell Cycle Assay, Colony Assay, Staining