hlif (Millipore)
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hlif
Hlif, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hlif/hlif/us12351831-709-28-29
Average 90 stars, based on 1 article reviews
Hlif, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hlif/hlif/us12351831-709-28-29
Average 90 stars, based on 1 article reviews
hlif - by Bioz Stars,
2026-09
90/100 stars
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Modification:Article Title: Substrate Stiffness Modulates Stemness and Differentiation of Rabbit Corneal Endothelium Through the Paxillin–YAP Pathway Article Snippet: The clusters of CECs were cultured in Opti-MEM I medium (31985070; Gibco) supplemented with 8% fetal bovine serum (10099141C; Gibco), 200 mg/L calcium chloride (C5670; Sigma-Aldrich, St. Louis, MO, USA), 50 μg/mL BPE (02-104; Upstate, Darmstadt, Germany), 20 μg/mL ascorbic acid (A4544; Sigma-Aldrich), 5 ng/mL human epidermal growth factor (E9644; Sigma-Aldrich), 0.08% chondroitin sulfate (C9819; Sigma-Aldrich), and 50 μg/mL penicillin–streptomycin (15140122; Gibco). .. After 24 hours, medium was replaced with a serum-free modified embryonic stem cell medium composed of DMEM/F12 (11320033; Gibco) with 10% knockout serum (10828010; Gibco), 4 ng/mL fibroblast growth factor–basic (100-18B; PeproTech, Cranbury, NJ, USA), 5 μg/mL ITS (41400045; Gibco), 10 ng/mL Knock-Out:Article Title: Substrate Stiffness Modulates Stemness and Differentiation of Rabbit Corneal Endothelium Through the Paxillin–YAP Pathway Article Snippet: The clusters of CECs were cultured in Opti-MEM I medium (31985070; Gibco) supplemented with 8% fetal bovine serum (10099141C; Gibco), 200 mg/L calcium chloride (C5670; Sigma-Aldrich, St. Louis, MO, USA), 50 μg/mL BPE (02-104; Upstate, Darmstadt, Germany), 20 μg/mL ascorbic acid (A4544; Sigma-Aldrich), 5 ng/mL human epidermal growth factor (E9644; Sigma-Aldrich), 0.08% chondroitin sulfate (C9819; Sigma-Aldrich), and 50 μg/mL penicillin–streptomycin (15140122; Gibco). .. After 24 hours, medium was replaced with a serum-free modified embryonic stem cell medium composed of DMEM/F12 (11320033; Gibco) with 10% knockout serum (10828010; Gibco), 4 ng/mL fibroblast growth factor–basic (100-18B; PeproTech, Cranbury, NJ, USA), 5 μg/mL ITS (41400045; Gibco), 10 ng/mL Article Title: In vitro production of expanded potential stem cells Article Snippet: Materials and Methods Culturing Mouse EPSCs, ESCs and iPSCs Mouse ESCs were cultured in standard N2B27-2i/LIF, or in M15 media-Knockout DMEM (Invitrogen), 15% foetal bovine serum (FBS, Hyclone), 1× Glutamine-Penicillin-Streptomycin (GPS, Invitrogen), 1× non-essential amino acids (NEAA, Invitrogen), 0.1 mM β-mercaptoethanol (β-ME, Sigma) and 106 U/ml hLIF (Millipore). .. Mouse EPSCs were cultured in EPSCM—DMEM/F12 (Invitrogen), 20% Knockout Serum Replacement (Invitrogen), 1× GPS, 1×NEAA, 0.1 mM β-ME and 106 U/ml Article Title: Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation. Article Snippet: Zhang et al., STAR Protocols 6, 103633 March 21, 2025 a 2025 The In vitro models of neuronal aging and gene manipulation in human neurons (hNeurons) are valuable tools for investigating brain aging and disease.. Here, we present a protocol for applying human embryonic stem cell (hESC)-derived neurons tomodel aging and the further application of small interfering RNA (siRNA)-mediated gene silencing for functional investigations.. We describe steps for neuronal differentiation and culture, siRNA transfection, and technical considerations to ensure reproducibility. Synthesized:Article Title: Platform for the induction and maintenance of ground state pluripotency Article Snippet: .. FRM consists of conventional medium (described above) supplemented with 5 μM Thiazovivin (synthesized in-house), 0.4 μM PD0325901 (BIOVISION), 1 μM CHIR99021 (BIOVISION), 2 μM SB431542 (BIOVISION), 100 ng/mL bFGF (LIFE TECHNOLOGIES), 10 ng/ml Article Title: Platform for the induction and maintenance of ground state pluripotency Article Snippet: Conventional medium consists of DMEM/F12 (MEDIATECH), 20% Knock-Out Serum Replacement (LIFE TECHNOLOGIES), 1× Glutagro (MEDIATECH), 1× Non-Essential Amino Acids (NEAA) (MEDIATECH), 1× Pen/Strep (MEDIATECH), and 100 μM β-Mercaptoethanol. .. FMM consists of conventional medium supplemented with 5 μM Thiazovivin (synthesized in-house), 0.4 μM PD0325901 (BIOVISION), 1 μM CHIR99021 (BIOVISION), 100 ng/ml bFGF (LIFE TECHNOLOGIES), and 10 ng/ml Cell Culture:Article Title: In vitro production of expanded potential stem cells Article Snippet: Materials and Methods Culturing Mouse EPSCs, ESCs and iPSCs Mouse ESCs were cultured in standard N2B27-2i/LIF, or in M15 media-Knockout DMEM (Invitrogen), 15% foetal bovine serum (FBS, Hyclone), 1× Glutamine-Penicillin-Streptomycin (GPS, Invitrogen), 1× non-essential amino acids (NEAA, Invitrogen), 0.1 mM β-mercaptoethanol (β-ME, Sigma) and 106 U/ml hLIF (Millipore). .. Mouse EPSCs were cultured in EPSCM—DMEM/F12 (Invitrogen), 20% Knockout Serum Replacement (Invitrogen), 1× GPS, 1×NEAA, 0.1 mM β-ME and 106 U/ml Article Title: Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation. Article Snippet: Zhang et al., STAR Protocols 6, 103633 March 21, 2025 a 2025 The In vitro models of neuronal aging and gene manipulation in human neurons (hNeurons) are valuable tools for investigating brain aging and disease.. Here, we present a protocol for applying human embryonic stem cell (hESC)-derived neurons tomodel aging and the further application of small interfering RNA (siRNA)-mediated gene silencing for functional investigations.. We describe steps for neuronal differentiation and culture, siRNA transfection, and technical considerations to ensure reproducibility. Article Title: Gasdermin D Mediated Mitochondrial Metabolism Orchestrate Neurogenesis Through LDHA During Embryonic Development. Article Snippet: After 5 days, single cells were dissociated with Accutase (Thermo Fisher) and seeded onto Metrigelcoated plates. .. The cells were then cultured in human NPCs proliferation medium containing 50% Neurobasal medium (Gibco), 50% DMEMF/12 medium (Gibco), 0.5% GlutaMAX (Invitrogen), 2% B27 supplement without VA (Invitrogen), 1% N2 supplement (Invitrogen), 10 ng mL−1 Recombinant:Article Title: Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation. Article Snippet: Zhang et al., STAR Protocols 6, 103633 March 21, 2025 a 2025 The In vitro models of neuronal aging and gene manipulation in human neurons (hNeurons) are valuable tools for investigating brain aging and disease.. Here, we present a protocol for applying human embryonic stem cell (hESC)-derived neurons tomodel aging and the further application of small interfering RNA (siRNA)-mediated gene silencing for functional investigations.. We describe steps for neuronal differentiation and culture, siRNA transfection, and technical considerations to ensure reproducibility. Transfection:Article Title: Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation. Article Snippet: Zhang et al., STAR Protocols 6, 103633 March 21, 2025 a 2025 The In vitro models of neuronal aging and gene manipulation in human neurons (hNeurons) are valuable tools for investigating brain aging and disease.. Here, we present a protocol for applying human embryonic stem cell (hESC)-derived neurons tomodel aging and the further application of small interfering RNA (siRNA)-mediated gene silencing for functional investigations.. We describe steps for neuronal differentiation and culture, siRNA transfection, and technical considerations to ensure reproducibility. Quantitative RT-PCR:Article Title: Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation. Article Snippet: Zhang et al., STAR Protocols 6, 103633 March 21, 2025 a 2025 The In vitro models of neuronal aging and gene manipulation in human neurons (hNeurons) are valuable tools for investigating brain aging and disease.. Here, we present a protocol for applying human embryonic stem cell (hESC)-derived neurons tomodel aging and the further application of small interfering RNA (siRNA)-mediated gene silencing for functional investigations.. We describe steps for neuronal differentiation and culture, siRNA transfection, and technical considerations to ensure reproducibility. |