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Curcuminoids increase global 5hmC in leukemia cells. Graded concentrations of curcumin (Cur) or DMC were used to treat U937 ( a , b ) and <t>HL60</t> leukemia cells ( c , d ) for 48 h, followed by genomic DNA extraction and quantitation of global 5hmC as described in the methods. The data represent the mean of 3 replicates ± SD. * indicates a significant difference from the control at p < 0.05.
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Curcuminoids increase global 5hmC in leukemia cells. Graded concentrations of curcumin (Cur) or DMC were used to treat U937 ( a , b ) and HL60 leukemia cells ( c , d ) for 48 h, followed by genomic DNA extraction and quantitation of global 5hmC as described in the methods. The data represent the mean of 3 replicates ± SD. * indicates a significant difference from the control at p < 0.05.

Journal: International Journal of Molecular Sciences

Article Title: Curcuminoids Activate TET Enzymes and Increase DNA Hydroxymethylation and Active Demethylation in Leukemia Cells

doi: 10.3390/ijms27010310

Figure Lengend Snippet: Curcuminoids increase global 5hmC in leukemia cells. Graded concentrations of curcumin (Cur) or DMC were used to treat U937 ( a , b ) and HL60 leukemia cells ( c , d ) for 48 h, followed by genomic DNA extraction and quantitation of global 5hmC as described in the methods. The data represent the mean of 3 replicates ± SD. * indicates a significant difference from the control at p < 0.05.

Article Snippet: Human promyeloid leukemia (HL60) and human monocytic leukemia (U937) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in RPMI-1640 medium (Sigma-Aldrich, Milwaukee, WI, USA) containing 10% fetal bovine serum (FBS) and 2.5 mM L-glutamine.

Techniques: DNA Extraction, Quantitation Assay, Control

Curcuminoids increase TET enzymatic activity in leukemia cells. Graded concentrations of curcumin (Cur) or DMC were used to treat U937 ( a , b ) and HL60 leukemia cells ( c , d ) for 48 h, followed by nuclear protein extraction and quantitation of TET activity, as described in the methods. The data represent the mean of 3 replicates ± SD. * indicates significant difference from the control at p < 0.05.

Journal: International Journal of Molecular Sciences

Article Title: Curcuminoids Activate TET Enzymes and Increase DNA Hydroxymethylation and Active Demethylation in Leukemia Cells

doi: 10.3390/ijms27010310

Figure Lengend Snippet: Curcuminoids increase TET enzymatic activity in leukemia cells. Graded concentrations of curcumin (Cur) or DMC were used to treat U937 ( a , b ) and HL60 leukemia cells ( c , d ) for 48 h, followed by nuclear protein extraction and quantitation of TET activity, as described in the methods. The data represent the mean of 3 replicates ± SD. * indicates significant difference from the control at p < 0.05.

Article Snippet: Human promyeloid leukemia (HL60) and human monocytic leukemia (U937) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in RPMI-1640 medium (Sigma-Aldrich, Milwaukee, WI, USA) containing 10% fetal bovine serum (FBS) and 2.5 mM L-glutamine.

Techniques: Activity Assay, Protein Extraction, Quantitation Assay, Control