Journal: STAR Protocols
Article Title: Protocol for morphogen-guided differentiation of brain cell types using human induced pluripotent stem cells
doi: 10.1016/j.xpro.2025.104339
Figure Lengend Snippet: Characterization of the representative human HPCs (A) Flow cytometry on HPC markers, CD34, CD41, CD253a, CD45, and CD43. Cellular events were subjected to debris removal (top-left), doublet removal (top-middle), and stained with DAPI (top-right) to gate for the analysis of viable cells. A combination of surface markers was used to evaluate the HPC identity (bottom panel) with expression of 92.1% CD34+/CD43+/CD253a+/CD41+/CD45-cells. (B) Representative images of HPC differentiation from Day 7 (D7) HPC, Day 14 (D14), Day 19 (D19) microglia to Day 38 (D38) mature microglia. Scale bar: 100 μm.
Article Snippet: CD45 (HI30) (1:50) , Tonbo Bioscience , Cat# 25-0459, RRID: AB_2621631.
Techniques: Flow Cytometry, Staining, Expressing