hescs  (MedChemExpress)

Journal: bioRxiv

Article Title: Increased chromatin accessibility following 1α,25-dihydroxyvitamin D 3 treatment in human endometrial stromal cells

doi: 10.64898/2026.05.06.723064

Figure Lengend Snippet: The analysis shows that 1,25(OH) 2 D 3 treatment is associated with increased accessibility signal without widespread reorganization of the global accessible chromatin landscape. (A) Overall profiles and heatmaps of ATAC-seq signal centered on called accessible regions (center ± 2 kb) and (B) surrounding transcription start sites (TSS ± 2 kb) in vehicle- and 1,25(OH) 2 D 3 -treated T-HESCs. (C) Overlap of open chromatin peaks identified by ATAC-seq in vehicle- and 1,25(OH) 2 D 3 -treated cells. (D) Representative genome browser loci of ATAC-seq signal at VDR, CYP24A1, PRL, and EFL1 regions from vehicle- and 1,25(OH) 2 D 3 -treated cells. RefSeq gene models are shown below.

Article Snippet: hTERT-immortalized endometrial stromal cells (T-HESCs) were purchased from the American Type Culture Collection (ATCC; Cat. CRL-4003, VA, USA).

Techniques:

Journal: bioRxiv

Article Title: Increased chromatin accessibility following 1α,25-dihydroxyvitamin D 3 treatment in human endometrial stromal cells

doi: 10.64898/2026.05.06.723064

Figure Lengend Snippet: (A) Clustered heatmaps and average signal profiles comparing ATAC-seq intensity patterns between vehicle- and 1,25(OH) 2 D 3 -treated cells across DARs. (B) Volcano plot showing differential chromatin accessibility between 1,25(OH) 2 D 3 -treated and vehicle-treated T-HESCs. Peaks were classified as increased or decreased accessible regions based on adjusted p -value and fold-change thresholds. (C) Comparison of genomic features of regions between loss and gain of accessibility. (D) Metaplot and heatmap of ATAC-seq signal across regions with increased accessibility in response to 1,25(OH) 2 D 3 , showing elevated signal intensity in ligand-treated cells compared with vehicle-treated cells. (E) Metaplot and heatmap of ATAC-seq signal across regions with decreased accessibility in response to 1,25(OH) 2 D 3 . The result shows that 1,25(OH) 2 D 3 induces a distinct set of accessibility changes, with pronounced signal enhancement at selected ligand-responsive regions.

Article Snippet: hTERT-immortalized endometrial stromal cells (T-HESCs) were purchased from the American Type Culture Collection (ATCC; Cat. CRL-4003, VA, USA).

Techniques: Comparison

Journal: bioRxiv

Article Title: Increased chromatin accessibility following 1α,25-dihydroxyvitamin D 3 treatment in human endometrial stromal cells

doi: 10.64898/2026.05.06.723064

Figure Lengend Snippet: (A) The number of overlapped peaks between ATAC-seq and publicly available CUT&RUN. (B) Enriched binding motifs from cistromic modification peaks within the open chromatin areas in T-HESCs by HOMER de novo analysis. (C) Venn diagram showing overlap of genes annotated to ATAC-seq peaks in vehicle- and 1,25(OH) 2 D 3 -treated T-HESCs. Peaks were annotated to the nearest gene within 100 kb of the transcription start site. A total of 19,789 genes were associated with open chromatin regions in vehicle-treated cells, whereas 19,114 genes were associated with open chromatin regions in 1,25(OH) 2 D 3 -treated cells. Most annotated genes were shared between conditions, with 18,322 genes common to both datasets. (D) Overlap between 1,25(OH) 2 D 3 -responsive DEGs and genes associated with open chromatin regions in vehicle- or 1,25(OH) 2 D 3 -treated cells. Among 626 DEGs, 540 overlapped with vehicle-associated open chromatin genes, whereas 530 overlapped with 1,25(OH) 2 D 3 -associated open chromatin genes. The high proportion of overlap indicates that most ligand-responsive transcripts are associated with nearby accessible chromatin regions present in either condition. (E) Ingenuity Pathway Analysis (IPA) of the 530 differentially expressed genes associated with open chromatin regions in 1,25(OH) 2 D 3 -treated T-HESCs. Causal network analysis identified VDR as the most significant predicted activated master regulator. (F) IPA canonical pathway analysis of the same gene set revealed enrichment of pathways related to transcriptional regulation, immune-associated signaling, and vitamin D receptor activity.

Article Snippet: hTERT-immortalized endometrial stromal cells (T-HESCs) were purchased from the American Type Culture Collection (ATCC; Cat. CRL-4003, VA, USA).

Techniques: Binding Assay, Modification, Activity Assay

Journal: bioRxiv

Article Title: Increased chromatin accessibility following 1α,25-dihydroxyvitamin D 3 treatment in human endometrial stromal cells

doi: 10.64898/2026.05.06.723064

Figure Lengend Snippet: (A) Summarized transcript information on the overlap between DARs. (B) Upregulated DARs overlap with 1,25(OH) 2 D 3 DEGs, and downregulated DARs overlap with 1,25(OH) 2 D 3 DEGs. (C) Representative ligand-responsive accessible regions selected for locus-specific quantitative validation. Corresponding validation of the enhancer peak region of GPAT3 and MAMDC2. (D) Proposed working model of 1,25(OH) 2 D 3 -dependent chromatin-transcription coupling in T-HESCs. 1,25(OH) 2 D 3 reinforces a permissive chromatin landscape in human endometrial stromal cells.

Article Snippet: hTERT-immortalized endometrial stromal cells (T-HESCs) were purchased from the American Type Culture Collection (ATCC; Cat. CRL-4003, VA, USA).

Techniques: Biomarker Discovery

Journal: Human Mutation

Article Title: Single‐Cell Transcriptomic Profiling and Machine Learning Integration Unveil Stromal Cell Heterogeneity in Endometriosis

doi: 10.1155/humu/5565366

Figure Lengend Snippet: Differential expression of key genes in ectopic endometrial cells. qRT‐PCR validation of four key genes (HOXA10, ESR1, MMP9, and SPP1) in normal endometrial stromal cells (NESCs) and ectopic endometrial stromal cells (EESCs). Gene expression was normalized to GAPDH and presented as fold change relative to NESCs. Data are shown as mean ± SD ( n = 3). ∗∗∗ p < 0.001 versus NESCs.

Article Snippet: NESCs (ATCC Cat# CRL‐4003, RRID:CVCL_D697) and EESCs (ATCC Cat# CRL‐7566, RRID:CVCL_IW41) were authenticated by short tandem repeat (STR) profiling, showing ≥ 95% match to the reference profile in the ATCC database.

Techniques: Quantitative Proteomics, Quantitative RT-PCR, Biomarker Discovery, Gene Expression

Journal: Human Mutation

Article Title: Single‐Cell Transcriptomic Profiling and Machine Learning Integration Unveil Stromal Cell Heterogeneity in Endometriosis

doi: 10.1155/humu/5565366

Figure Lengend Snippet: Differential expression of key genes in ectopic endometrial cells. qRT‐PCR validation of four key genes (HOXA10, ESR1, MMP9, and SPP1) in normal endometrial stromal cells (NESCs) and ectopic endometrial stromal cells (EESCs). Gene expression was normalized to GAPDH and presented as fold change relative to NESCs. Data are shown as mean ± SD ( n = 3). ∗∗∗ p < 0.001 versus NESCs.

Article Snippet: NESCs (ATCC Cat# CRL‐4003, RRID:CVCL_D697) and EESCs (ATCC Cat# CRL‐7566, RRID:CVCL_IW41) were authenticated by short tandem repeat (STR) profiling, showing ≥ 95% match to the reference profile in the ATCC database.

Techniques: Quantitative Proteomics, Quantitative RT-PCR, Biomarker Discovery, Gene Expression