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hdac6  (ATCC)


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    Structured Review

    ATCC hdac6
    Hdac6, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1081 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hdac6/product/ATCC
    Average 97 stars, based on 1081 article reviews
    hdac6 - by Bioz Stars, 2026-03
    97/100 stars

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    Primary human monocyte-derived macrophages (hMDM) were treated with dopamine (10 -6 M) for 3 hours, and gene expression was assessed by qPCR. ( A ) Dopamine significantly increased HDAC2 mRNA expression (n=17 donors, *p<0.05). ( B ) Dopamine significantly increased HDAC6 mRNA expression (n=14 donors, *p<0.05). ( C ) KAT2A mRNA expression was not significantly altered by dopamine treatment(n=17 donors). ( D ) KDM6B mRNA expression was not significantly altered by dopamine treatment (n=17 donors).

    Journal: bioRxiv

    Article Title: Epigenetic Regulation of Inflammation by Dopamine in Primary Human Macrophages

    doi: 10.64898/2026.01.21.700899

    Figure Lengend Snippet: Primary human monocyte-derived macrophages (hMDM) were treated with dopamine (10 -6 M) for 3 hours, and gene expression was assessed by qPCR. ( A ) Dopamine significantly increased HDAC2 mRNA expression (n=17 donors, *p<0.05). ( B ) Dopamine significantly increased HDAC6 mRNA expression (n=14 donors, *p<0.05). ( C ) KAT2A mRNA expression was not significantly altered by dopamine treatment(n=17 donors). ( D ) KDM6B mRNA expression was not significantly altered by dopamine treatment (n=17 donors).

    Article Snippet: TaqMan Fast Universal Master Mix, and PCR assay probes for DRD1-5 (Hs00265245_s1, Hs00241436_m1, Hs00364455_m1, Hs00609526_m1, Hs00361234_s1), IL1B (Hs01555410_m1), DNMT1 (Hs00945875_m1), DNMT3A (Hs01027166_m1), DNMT3B (Hs00171876_m1), TET2 (Hs00325999_m1), HDAC2 (Hs00231032_m1), HDAC6 (Hs00997427_m1), KDM6B (Hs00996325_g1), KAT2A (Hs00904943_gH), and 18S (4319413E) genes were purchased from Applied Biosystems.

    Techniques: Derivative Assay, Gene Expression, Expressing

    Data from were stratified by donor sex and age to assess potential sources of inter-donor variability. ( A ) Dopamine significantly increased HDAC2 mRNA expression in hMDMs from female donors (n=10, *p<0.05) but not male donors (n=7). ( B ) Dopamine treatment showed an increasing trend in HDAC6 expression in male hMDMs (n=9; p=0.0742) and a non-significant increase in female hMDMs (n=5). Dopamine treatment did not reveal sex-associated differences in ( C ) KAT2A or ( D ) KDM6B gene expression (n=17 donors each). ( E ) Dopamine treatment showed an increasing trend in HDAC2 expression in donors under 40 years of age (n=8, p=0.0547), but not in donors aged 40 years or older (n=9). No age-associated differences were observed for dopamine-induced changes in ( F ) HDAC6 (n=14), ( G ) KAT2A (n=17), or ( H ) KDM6B (n=17) expression.

    Journal: bioRxiv

    Article Title: Epigenetic Regulation of Inflammation by Dopamine in Primary Human Macrophages

    doi: 10.64898/2026.01.21.700899

    Figure Lengend Snippet: Data from were stratified by donor sex and age to assess potential sources of inter-donor variability. ( A ) Dopamine significantly increased HDAC2 mRNA expression in hMDMs from female donors (n=10, *p<0.05) but not male donors (n=7). ( B ) Dopamine treatment showed an increasing trend in HDAC6 expression in male hMDMs (n=9; p=0.0742) and a non-significant increase in female hMDMs (n=5). Dopamine treatment did not reveal sex-associated differences in ( C ) KAT2A or ( D ) KDM6B gene expression (n=17 donors each). ( E ) Dopamine treatment showed an increasing trend in HDAC2 expression in donors under 40 years of age (n=8, p=0.0547), but not in donors aged 40 years or older (n=9). No age-associated differences were observed for dopamine-induced changes in ( F ) HDAC6 (n=14), ( G ) KAT2A (n=17), or ( H ) KDM6B (n=17) expression.

    Article Snippet: TaqMan Fast Universal Master Mix, and PCR assay probes for DRD1-5 (Hs00265245_s1, Hs00241436_m1, Hs00364455_m1, Hs00609526_m1, Hs00361234_s1), IL1B (Hs01555410_m1), DNMT1 (Hs00945875_m1), DNMT3A (Hs01027166_m1), DNMT3B (Hs00171876_m1), TET2 (Hs00325999_m1), HDAC2 (Hs00231032_m1), HDAC6 (Hs00997427_m1), KDM6B (Hs00996325_g1), KAT2A (Hs00904943_gH), and 18S (4319413E) genes were purchased from Applied Biosystems.

    Techniques: Expressing, Gene Expression

    Donors from were combined with an independent archival cohort to assess correlations between dopamine receptor expression and epigenetic enzyme expression by qPCR. DRD1 expression was significantly correlated with ( A ) HDAC2 (n=28, **p<0.01), ( B ) HDAC6 (n=25, **p<0.01), ( C ) KAT2A (n=28, **p<0.01), and ( D ) KDM6B expression (n=28, ***p<0.001). DRD5 expression was significantly correlated with ( E ) HDAC2 (n=28, *p<0.01), ( F ) HDAC6 (n=25, *p<0.01), and ( H ) KDM6B (n=28, *p<0.05), but not with ( G ) KAT2A expression (n=28, p=0.0733).

    Journal: bioRxiv

    Article Title: Epigenetic Regulation of Inflammation by Dopamine in Primary Human Macrophages

    doi: 10.64898/2026.01.21.700899

    Figure Lengend Snippet: Donors from were combined with an independent archival cohort to assess correlations between dopamine receptor expression and epigenetic enzyme expression by qPCR. DRD1 expression was significantly correlated with ( A ) HDAC2 (n=28, **p<0.01), ( B ) HDAC6 (n=25, **p<0.01), ( C ) KAT2A (n=28, **p<0.01), and ( D ) KDM6B expression (n=28, ***p<0.001). DRD5 expression was significantly correlated with ( E ) HDAC2 (n=28, *p<0.01), ( F ) HDAC6 (n=25, *p<0.01), and ( H ) KDM6B (n=28, *p<0.05), but not with ( G ) KAT2A expression (n=28, p=0.0733).

    Article Snippet: TaqMan Fast Universal Master Mix, and PCR assay probes for DRD1-5 (Hs00265245_s1, Hs00241436_m1, Hs00364455_m1, Hs00609526_m1, Hs00361234_s1), IL1B (Hs01555410_m1), DNMT1 (Hs00945875_m1), DNMT3A (Hs01027166_m1), DNMT3B (Hs00171876_m1), TET2 (Hs00325999_m1), HDAC2 (Hs00231032_m1), HDAC6 (Hs00997427_m1), KDM6B (Hs00996325_g1), KAT2A (Hs00904943_gH), and 18S (4319413E) genes were purchased from Applied Biosystems.

    Techniques: Expressing