Journal: iScience
Article Title: Jchain -diphtheria toxin receptor mice allow for depletion of antibody-secreting cells and analysis of differentiation kinetics
doi: 10.1016/j.isci.2025.113946
Figure Lengend Snippet: Validation of DTR gene expression by ASCs from J-DTR mice (A) Representative flow cytometry pseudocolor plots showing gating of CD19 + CD138 -/LO B cells and CD138 HI CD267(TACI) + ASCs in total spleen or cells purified with STEMCELL Technologies Pan-B and CD138 (ASC) isolation kits. Numbers in plots indicate percentages of gated populations within total live singlets. (B) Quantification of ASC percentages in total spleen or purified cells using Pan-B and ASC isolation kits. (C–E) Relative gene expression of (C) Prdm1 , (D) Jchain , and (E) DTR ( HBEGF ) in cells purified using Pan-B and ASC isolation kits. All values are relative to the expression of Actb . (F) DTR expression normalized to Prdm1 expression in WT and J-DTR ASCs. (G) Jchain expression normalized to Prdm1 expression in WT and J-DTR ASCs. (B–G) Symbols represent individual 3–7 months old female (orange) and male (blue) mice. Data pooled from 3 independent experiments. Horizontal lines represent mean ± standard error of the mean (SEM). WT spleen, Pan-B and ASC: female n = 2, male n = 3; J-DTR spleen, Pan-B and ASC: female n = 3, male n = 3. (B) Statistics: One-way ANOVA with Dunnett’s multiple comparisons test with ASCs for each genotype set as the control column. (C–G) Statistics: Unpaired Student’s t test between genotypes or cell types (related to and ).
Article Snippet: TaqMan Human HBEGF (DTR) (Hs00961129_m1) , Thermo Fisher Scientific , Cat# 4331182.
Techniques: Biomarker Discovery, Gene Expression, Flow Cytometry, Purification, Isolation, Expressing, Control
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