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hbdsmc  (Lonza)


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    Lonza hbdsmc
    Hbdsmc, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hbdsmc/hbdsmc/pmc11592815-26-45-70
    Average 90 stars, based on 1 article reviews
    hbdsmc - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Transmembrane-4 L-Six Family Member-1 Is Essential for Embryonic Blood Vessel Development
    Article Snippet: All seven human primary endothelial cells (ECs), HUVEC (human umbilical vein EC), HLMEC (lung microvascular EC), HDMEC, (human dermal microvascular EC), HCAEC (human coronary artery EC), HPAEC (human pulmonary artery EC), ECFCs (endothelial colony forming cells), HMVEC-dLy (human dermal microvascular lymphatic EC), as well as HBdSMC (smooth muscle cells, bladder), HDF (fibroblast, dermal), HemaLP (epidermal melanocytes), and bone marrow-derived mesenchymal stem cells were purchased along with their respective media from Lonza (Walkersville, MD, USA), and used within six passages in all experiments conducted in this study.



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    Image Search Results


    RNA-seq analysis of total DEGs for YAP-KD hBdSMC. (A) . The heatmap of YAP-KD and NC hBdSMCs. (B) . The volcano plot of DEGs. (C) . KEGG analysis of DEGs. (D) . GO analysis of DEGs. (E) . The PPI network of DEGs. hBdSMC: human bladder smooth muscle cell; KD: knock-down, NC: nature control, DEG: differentially expressed genes, PPI: protein-protein interaction.

    Journal: Frontiers in Genetics

    Article Title: YAP-mediated mechanotransduction in urinary bladder remodeling: Based on RNA-seq and CUT&Tag

    doi: 10.3389/fgene.2023.1106927

    Figure Lengend Snippet: RNA-seq analysis of total DEGs for YAP-KD hBdSMC. (A) . The heatmap of YAP-KD and NC hBdSMCs. (B) . The volcano plot of DEGs. (C) . KEGG analysis of DEGs. (D) . GO analysis of DEGs. (E) . The PPI network of DEGs. hBdSMC: human bladder smooth muscle cell; KD: knock-down, NC: nature control, DEG: differentially expressed genes, PPI: protein-protein interaction.

    Article Snippet: hBdSMCs cell line was purchased from ScienCell, United States (Cat No.4310), which was cultured with SMCM medium (ScienCell, United States, Cat No.1102) with fetal bovine serum (10%), streptomycin (100 μg/mL), penicillin (100 U/mL), and growth factor.

    Techniques: RNA Sequencing, Knockdown, Control

    YAP is the key regulator in ECM stiffness‐induced hBdSMCs proliferation. (A) Proliferating cell nuclear antigen (PCNA) protein of cells with YAP knocked down under different stiffness was detected by western blot, n = 3. KD‐YAP, Knock down of YAP. Scrambled1 is for KD‐YAP. (B) PCNA was detected by Western blot under 32 kPa in rescue experiment, n = 3. Ad‐YAP, over‐expression of YAP. Scrambled1 is for KD‐YAP, Scrambled2 is for Ad‐YAP. (C) EdU assay of KD‐YAP under different stiffness, scale bar = 200 µm. (D) Quantification of EdU assay in (C) is the mean ± SEM, n = 10, ** P < 0.01, **** P < 0.0001. (E) EdU assay of rescue experiment under 32 kPa was performed to detect the DNA replication, scale bar = 200 µm. Scrambled1 is for KD‐YAP, Scrambled2 is for Ad‐YAP. (F) Quantification of the EdU assay in (E) is the mean ± SEM, n = 5, * P < 0.05, **** P < 0.0001

    Journal: MedComm

    Article Title: YAP/Smad3 promotes pathological extracellular matrix microenviroment‐induced bladder smooth muscle proliferation in bladder fibrosis progression

    doi: 10.1002/mco2.169

    Figure Lengend Snippet: YAP is the key regulator in ECM stiffness‐induced hBdSMCs proliferation. (A) Proliferating cell nuclear antigen (PCNA) protein of cells with YAP knocked down under different stiffness was detected by western blot, n = 3. KD‐YAP, Knock down of YAP. Scrambled1 is for KD‐YAP. (B) PCNA was detected by Western blot under 32 kPa in rescue experiment, n = 3. Ad‐YAP, over‐expression of YAP. Scrambled1 is for KD‐YAP, Scrambled2 is for Ad‐YAP. (C) EdU assay of KD‐YAP under different stiffness, scale bar = 200 µm. (D) Quantification of EdU assay in (C) is the mean ± SEM, n = 10, ** P < 0.01, **** P < 0.0001. (E) EdU assay of rescue experiment under 32 kPa was performed to detect the DNA replication, scale bar = 200 µm. Scrambled1 is for KD‐YAP, Scrambled2 is for Ad‐YAP. (F) Quantification of the EdU assay in (E) is the mean ± SEM, n = 5, * P < 0.05, **** P < 0.0001

    Article Snippet: hBdSMCs (ScienCell, USA, Cat No. 4310) were cultured with SMCM medium (ScienCell, USA, Cat. No. 1102) containing fetal bovine serum (10%), treptomycin (100 μg/ml), and penicillin (100 U/ml), and smooth muscle cell growth factor., The 293T cells supplied by Genechem, Shanghai, were cultured with Dulbecco's modified Eagle medium (DMEM) containing fetal bovine serum (10%), treptomycin (100 μg/ml), and penicillin (100 U/ml).

    Techniques: Western Blot, Knockdown, Over Expression, EdU Assay