hadha (Proteintech)

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Proteintech hadha
Hadha, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hadha/HADHA+Antibody/pm41872163-137-27-29
Average 94 stars, based on 38 article reviews

hadha - by Bioz Stars, 2026-10

94/100 stars

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Incubation:

Article Title: SIRT1 deficiency promotes age-related heart failure through enhancing ferroptosis via GATA4-HADHA-GPX4 axis.
Article Snippet: Proteins were transferred to PVDF membranes (Millipore, Billerica, MA, USA). .. The membranes were then incubated with primary antibodies against TF (1:500, Proteintech, cat#17435-1-AP, RRID: AB_2035023), FTH1 (1:500, Bioss, cat#bs-5907R, RRID: AB_11050926), GPX4 (1:500, Abcam, cat#ab125066, RRID: AB_10973901), HADHA (1:2000, Proteintech, cat#10758-1-AP, RRID: AB_2115593), and SIRT1 (IP, 1:500, Proteintech, cat#60303-1-Ig, RRID: AB_2881417; IB, 1:500, Abcam, cat#ab189494, RRID: AB_2864311), GATA4 (1:500, Proteintech, cat#19530-1-AP, RRID: AB_10642003), and GAPDH (1:10000, Abcam, cat#ab128915, RRID: AB_2747414) at 4°C overnight. .. After washing, the membranes were incubated with anti-IgG horseradish peroxidase-conjugated secondary antibodies (Jackson Immuno Research, West Grove, PA, USA).

Article Title: Very high-dose vitamin D₃ supplementation reduces the expression of genes and proteins engaged in β-oxidation in healthy pigs.
Article Snippet: For the Western blotting, 10 μg of protein samples were separated by 12 % SDS/PAGE gel and transferred with semi-wet transfer onto a polyvinylidene difluoride (PVDF) membrane (Trans-Blot Turbo System, Bio-Rad, USA). .. The membrane was blocked with 3 % BSA (Serva, Germany) in TBS containing 0.1 % Tween-20 (TBS-T) for one h at room temperature and then incubated with primary antibody against LDHB, HADHA, CRP, RGN, IDH1, ASL, ACSL5, ACADM, TPI1, β-tubulin (Proteintech, Germany), or β-actin (Invitrogen, USA) dissolved in blocking buffer, overnight at 4 ◦C. .. The blots were then washed in TBS-T three times and incubated with HRP-labeled secondary antibody (Thermo Scientific, USA) for 1 h at room temperature.

Article Title: SIRT1 deficiency promotes age-related heart failure through enhancing ferroptosis via GATA4-HADHA-GPX4 axis
Article Snippet: Proteins were transferred to PVDF membranes (Millipore, Billerica, MA, USA). .. The membranes were then incubated with primary antibodies against TF (1:500, Proteintech, cat#17435-1-AP, RRID: AB_2035023), FTH1 (1:500, Bioss, cat#bs-5907R, RRID: AB_11050926), GPX4 (1:500, Abcam, cat#ab125066, RRID: AB_10973901), HADHA (1:2000, Proteintech, cat#10758-1-AP, RRID: AB_2115593), and SIRT1 (IP, 1:500, Proteintech, cat#60303-1-Ig, RRID: AB_2881417; IB, 1:500, Abcam, cat#ab189494, RRID: AB_2864311), GATA4 (1:500, Proteintech, cat#19530-1-AP, RRID: AB_10642003), and GAPDH (1:10000, Abcam, cat#ab128915, RRID: AB_2747414) at 4°C overnight. .. After washing, the membranes were incubated with anti-IgG horseradish peroxidase-conjugated secondary antibodies (Jackson Immuno Research, West Grove, PA, USA).

Membrane:

Blocking Assay:

Control:

Article Title: Putative Role of Fatty Acid Metabolic Therapy Using Ketogenic Diet and HIF-1α Inhibition in Hepatocellular Carcinoma: Evidence from an In Vitro Study
Article Snippet: .. The primary antibodies used were HIF-1α (1:1000; cat. no. 610959; BD Biosciences, CA, USA), HEL (Hexanoyl Lysine; 1:1000; cat. no. MHL-021P; JaICA, Shizuoka, Japan), cleaved caspase-3 (1:1000; cat. no. 9661; Cell Signaling Technology, Danvers, MA, USA), ACSL1 (acyl-CoA synthetase long-chain family member 1; 1:1000; cat. no. 13989-1-AP; Proteintech, Rosemont, IL, USA), CPT1A (carnitine palmitoyltransferase 1A; 1:1000; cat. no. 12252; Cell Signaling Technology), MCAD (medium-chain acyl-CoA dehydrogenase; 1:1000; cat. no. 55210-1-AP; Proteintech), LCAD (long-chain acyl-CoA dehydrogenase; 1:2000; cat. no. 17442-1-AP; Proteintech), HADHA (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha; 1:50,000; cat. no. 10758-1-AP; Proteintech), HADHB (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta; 1:12,000; cat. no. 29091-1-AP; Proteintech), ECHS1 (enoyl-CoA hydratase, short chain 1; 1:20,000; cat. no. 29091-1-AP; Proteintech), BDH1 (3-hydroxybutyrate dehydrogenase type 1; 1:10,000; cat. no. 67448-1-lg; Proteintech), HMGCS2 (3-hydroxy-3-methylglutaryl-CoA synthase 2; 1:2000; cat. no. 20940; Cell Signaling Technology), HMGCL (3-hydroxy-3-methylglutaryl-CoA lyase; 1:2000; cat. no. ab197022; abcam, Cambridge, UK), MCT4 (monocarboxylate transporter 4; 1:100,000; cat. no. 22787-1-AP; Proteintech), MCT1 (monocarboxylate transporter 1; 1:3000; cat. no. 85680; Cell Signaling Technology), OXCT1 (3-oxoacid CoA-transferase 1; 1:30,000; cat. no. 67836-1-lg; Proteintech), ACAT1 (acetyl-CoA acetyltransferase 1; 1:10,000; cat. no. 44276; Cell Signaling Technology); ACTB (β-actin; 1:10,000, cat. no. AC15; Sigma-Aldrich; Merck KGaA, MO, USA) was used as the internal control. ..

Article Title: Putative Role of Fatty Acid Metabolic Therapy Using Ketogenic Diet and HIF-1α Inhibition in Hepatocellular Carcinoma: Evidence from an In Vitro Study.
Article Snippet: .. The primary antibodies used were HIF-1α (1:1000; cat. no. 610959; BD Biosciences, CA, USA), HEL (Hexanoyl Lysine; 1:1000; cat. no. MHL-021P; JaICA, Shizuoka, Japan), cleaved caspase-3 (1:1000; cat. no. 9661; Cell Signaling Technology, Danvers, MA, USA), ACSL1 (acyl-CoA synthetase long-chain family member 1; 1:1000; cat. no. 13989-1-AP; Proteintech, Rosemont, IL, USA), CPT1A (carnitine palmitoyltransferase 1A; 1:1000; cat. no. 12252; Cell Signaling Technology), MCAD (medium-chain acyl-CoA dehydrogenase; 1:1000; cat. no. 55210-1-AP; Proteintech), LCAD (long-chain acyl-CoA dehydrogenase; 1:2000; cat. no. 17442-1-AP; Proteintech), HADHA (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha; 1:50,000; cat. no. 10758-1-AP; Proteintech), HADHB (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta; 1:12,000; cat. no. 29091-1-AP; Proteintech), ECHS1 (enoyl-CoA hydratase, short chain 1; 1:20,000; cat. no. 29091-1-AP; Proteintech), BDH1 (3-hydroxybutyrate dehydrogenase type 1; 1:10,000; cat. no. 67448-1-lg; Proteintech), HMGCS2 (3-hydroxy-3methylglutaryl-CoA synthase 2; 1:2000; cat. no. 20940; Cell Signaling Technology), HMGCL (3-hydroxy-3-methylglutaryl-CoA lyase; 1:2000; cat. no. ab197022; abcam, Cambridge, UK), MCT4 (monocarboxylate transporter 4; 1:100,000; cat. no. 22787-1-AP; Proteintech), MCT1 (monocarboxylate transporter 1; 1:3000; cat. no. 85680; Cell Signaling Technology), OXCT1 (3-oxoacid CoA-transferase 1; 1:30,000; cat. no. 67836-1-lg; Proteintech), ACAT1 (acetyl-CoA acetyltransferase 1; 1:10,000; cat. no. 44276; Cell Signaling Technology); ACTB (β-actin; 1:10,000, cat. no. AC15; Sigma-Aldrich; Merck KGaA, MO, USA) was used as the internal control. ..

Review