Incubation:Article Title: Tryptophan Metabolism Acts as a New Anti‐Ferroptotic Pathway to Mediate Tumor Growth
Article Snippet: Total 20 μg proteins were separated by 10% SDS‐polyacrylamide gel, transferred onto nitrocellulose filter membrane (Millipore Corp, Billerica, MA, USA), blocked with 5% fat‐free milk for 1 h at room temperature. .. The primary antibodies against MAOA (1:1000; 10539‐1‐AP, Proteintech), MAOB (1:1000; 10602‐1‐AP, Proteintech), DDC (1:1000; ab131282, abcam), KYNU (1:1000; 11796‐1‐AP, Proteintech), HAAO (1:1000; 12791‐1‐AP, Proteintech), GPX4 (1:1000; ab125066, abcam), FSP1 (1:1000; 20886‐1‐AP, Proteintech), DHODH (1:1000; 14877‐1‐AP, Proteintech), GCH1 (1:1000; 28501‐1‐AP, Proteintech), SLC7A11 (1:1000; 12691S, CST), p‐AMPK α (1:1000; 2535S, CST), p‐mTOR (1:1000; 5536, CST), ACSL4 (1:1000; ab155282, abcam), 4‐HNE (1:1000; ab46545, abcam), NRF2 (1:1000; ab137550, abcam), 5‐HT1A (1:1000; AF0482, Affinity), 5‐HT1B (1:1000; DF3497, Affinity), 5‐HT1D (1:1000; DF2706, Affinity), 5‐HT1F (1:1000; DF3499, Affinity), 5‐HT2A (1:1000; DF8900, Affinity), 5‐HT2B (1:1000; DF3500, Affinity), 5‐HT2C (1:1000; ab133570, abcam), 5‐HT3A (1:1000; DF2707, Affinity), 5‐HT3B (1:1000; DF10195, Affinity), 5‐HT4 (1:1000; DF3503, Affinity), 5‐HT5A (1:1000; AF0631, Affinity), 5‐HT6 (1:1000; DF3505, Affinity), 5‐HT7 (1:1000; ab133570, abcam), Vinculin (1:1000; 66305‐1, Proteintech) and β ‐actin(1;3000; 20536, Proteintech) were incubated overnight at 4 °C, sequentially the peroxidase affinipure goat anti‐rabbit or mouse IgG(H+L) (Jackson ImmunoResearch Laboratories) and enhanced chemiluminescence solution (GE Healthcare) were used for visualizing protein expression. ..
Article Title: Tryptophan Metabolism Acts as a New Anti-Ferroptotic Pathway to Mediate Tumor Growth.
Article Snippet: Total 20 μg proteins were separated by 10% SDS-polyacrylamide gel, transferred onto nitrocellulose filter membrane (Millipore Corp, Billerica, MA, USA), blocked with 5% fat-free milk for 1 h at room temperature. .. The primary antibodies against MAOA (1:1000; 10539-1-AP, Proteintech), MAOB (1:1000; 10602-1-AP, Proteintech), DDC (1:1000; ab131282, abcam), KYNU (1:1000; 11796-1-AP, Proteintech), HAAO (1:1000; 12791-1-AP, Proteintech), GPX4 (1:1000; ab125066, abcam), FSP1 (1:1000; 20886-1-AP, Proteintech), DHODH (1:1000; 14877- 1-AP, Proteintech), GCH1 (1:1000; 28501-1-AP, Proteintech), SLC7A11 (1:1000; 12691S, CST), p-AMPKα (1:1000; 2535S, CST), p-mTOR (1:1000; 5536, CST), ACSL4 (1:1000; ab155282, abcam), 4-HNE (1:1000; ab46545, abcam), NRF2 (1:1000; ab137550, abcam), 5-HT1A (1:1000; AF0482, Affinity), 5-HT1B (1:1000; DF3497, Affinity), 5-HT1D (1:1000; DF2706, Affinity), 5-HT1F (1:1000; DF3499, Affinity), 5-HT2A (1:1000; DF8900, Affinity), 5- HT2B (1:1000; DF3500, Affinity), 5-HT2C (1:1000; ab133570, abcam), 5- HT3A (1:1000; DF2707, Affinity), 5-HT3B (1:1000; DF10195, Affinity), 5- HT4 (1:1000; DF3503, Affinity), 5-HT5A (1:1000; AF0631, Affinity), 5-HT6 (1:1000; DF3505, Affinity), 5-HT7 (1:1000; ab133570, abcam), Vinculin (1:1000; 66305-1, Proteintech) and β-actin(1;3000; 20536, Proteintech) were incubated overnight at 4 °C, sequentially the peroxidase affinipure goat anti-rabbit or mouse IgG(H+L) (Jackson ImmunoResearch Laboratories) and enhanced chemiluminescence solution (GE Healthcare) were used for visualizing protein expression. ..
Expressing:Article Title: Tryptophan Metabolism Acts as a New Anti‐Ferroptotic Pathway to Mediate Tumor Growth
Article Snippet: Total 20 μg proteins were separated by 10% SDS‐polyacrylamide gel, transferred onto nitrocellulose filter membrane (Millipore Corp, Billerica, MA, USA), blocked with 5% fat‐free milk for 1 h at room temperature. .. The primary antibodies against MAOA (1:1000; 10539‐1‐AP, Proteintech), MAOB (1:1000; 10602‐1‐AP, Proteintech), DDC (1:1000; ab131282, abcam), KYNU (1:1000; 11796‐1‐AP, Proteintech), HAAO (1:1000; 12791‐1‐AP, Proteintech), GPX4 (1:1000; ab125066, abcam), FSP1 (1:1000; 20886‐1‐AP, Proteintech), DHODH (1:1000; 14877‐1‐AP, Proteintech), GCH1 (1:1000; 28501‐1‐AP, Proteintech), SLC7A11 (1:1000; 12691S, CST), p‐AMPK α (1:1000; 2535S, CST), p‐mTOR (1:1000; 5536, CST), ACSL4 (1:1000; ab155282, abcam), 4‐HNE (1:1000; ab46545, abcam), NRF2 (1:1000; ab137550, abcam), 5‐HT1A (1:1000; AF0482, Affinity), 5‐HT1B (1:1000; DF3497, Affinity), 5‐HT1D (1:1000; DF2706, Affinity), 5‐HT1F (1:1000; DF3499, Affinity), 5‐HT2A (1:1000; DF8900, Affinity), 5‐HT2B (1:1000; DF3500, Affinity), 5‐HT2C (1:1000; ab133570, abcam), 5‐HT3A (1:1000; DF2707, Affinity), 5‐HT3B (1:1000; DF10195, Affinity), 5‐HT4 (1:1000; DF3503, Affinity), 5‐HT5A (1:1000; AF0631, Affinity), 5‐HT6 (1:1000; DF3505, Affinity), 5‐HT7 (1:1000; ab133570, abcam), Vinculin (1:1000; 66305‐1, Proteintech) and β ‐actin(1;3000; 20536, Proteintech) were incubated overnight at 4 °C, sequentially the peroxidase affinipure goat anti‐rabbit or mouse IgG(H+L) (Jackson ImmunoResearch Laboratories) and enhanced chemiluminescence solution (GE Healthcare) were used for visualizing protein expression. ..
Article Title: Tryptophan Metabolism Acts as a New Anti-Ferroptotic Pathway to Mediate Tumor Growth.
Article Snippet: Total 20 μg proteins were separated by 10% SDS-polyacrylamide gel, transferred onto nitrocellulose filter membrane (Millipore Corp, Billerica, MA, USA), blocked with 5% fat-free milk for 1 h at room temperature. .. The primary antibodies against MAOA (1:1000; 10539-1-AP, Proteintech), MAOB (1:1000; 10602-1-AP, Proteintech), DDC (1:1000; ab131282, abcam), KYNU (1:1000; 11796-1-AP, Proteintech), HAAO (1:1000; 12791-1-AP, Proteintech), GPX4 (1:1000; ab125066, abcam), FSP1 (1:1000; 20886-1-AP, Proteintech), DHODH (1:1000; 14877- 1-AP, Proteintech), GCH1 (1:1000; 28501-1-AP, Proteintech), SLC7A11 (1:1000; 12691S, CST), p-AMPKα (1:1000; 2535S, CST), p-mTOR (1:1000; 5536, CST), ACSL4 (1:1000; ab155282, abcam), 4-HNE (1:1000; ab46545, abcam), NRF2 (1:1000; ab137550, abcam), 5-HT1A (1:1000; AF0482, Affinity), 5-HT1B (1:1000; DF3497, Affinity), 5-HT1D (1:1000; DF2706, Affinity), 5-HT1F (1:1000; DF3499, Affinity), 5-HT2A (1:1000; DF8900, Affinity), 5- HT2B (1:1000; DF3500, Affinity), 5-HT2C (1:1000; ab133570, abcam), 5- HT3A (1:1000; DF2707, Affinity), 5-HT3B (1:1000; DF10195, Affinity), 5- HT4 (1:1000; DF3503, Affinity), 5-HT5A (1:1000; AF0631, Affinity), 5-HT6 (1:1000; DF3505, Affinity), 5-HT7 (1:1000; ab133570, abcam), Vinculin (1:1000; 66305-1, Proteintech) and β-actin(1;3000; 20536, Proteintech) were incubated overnight at 4 °C, sequentially the peroxidase affinipure goat anti-rabbit or mouse IgG(H+L) (Jackson ImmunoResearch Laboratories) and enhanced chemiluminescence solution (GE Healthcare) were used for visualizing protein expression. ..
Western Blot:Article Title: 3-HAA Metabolic Pathway Regulates HCC Growth
Article Snippet: .. Antibodies against the following proteins were used for immunoblotting: IDO1 (CST, cat: 86630), TDO2 (Origene, cat: TA504879), KYNU (Proteintech, cat: 11796-1-AP), KMO (Proteintech, cat: 10698-1-AP), HAAO (Proteintech, cat: 12791-1-AP), Cleaved Caspase3 (CST, cat:9315), PARP (Proteintech, cat: 13371-1-AP), DUSP6 (Proteintech, cat: 10433-1-AP), IGFBP1 (Proteintech, cat: 13981-1-AP), p-ERK (CST, cat: 4370), ERK (CST, cat: 4695), BAD (CST, cat: 9239), BCL2 (CST, cat: 4223), BCL-XL (CST, cat: 2764), YY1 (Active Motif, cat: 61779), H3 (Proteintech, cat: 17168-1-AP), p-YY1 Page 5/24 (Abclonal, cat: E13449), HA (Sangon, cat: D110004), PKCζ (CST, cat: 9368), RIP3 (Proteintech, cat: 17563- 1-AP), P62 (CST, cat: 5114), LC3B (CST, cat: 2775), CYP1A1 (Proteintech, cat: 13241-1-AP), CYP1B1 (Proteintech, cat: 18505-1-AP), TIPARP (Sigma, cat:SAB2102431) and β-actin (Santa Cruz, cat: 47778). .. The immunoblots were scanned using an Odyssey infrared imaging system (LI-COR).
Article Title: Kynurenine catabolic enzyme KMO regulates HCC growth
Article Snippet: For nuclear and cytoplasmic protein analysis, the Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime, cat: P0028) was used according to the instructions. .. Antibodies against the following proteins were used for immunoblotting: IDO1 (CST, cat: 86630), TDO2 (Origene, cat: TA504879), KYNU (Proteintech, cat: 11796-1-AP), KMO (Proteintech, cat: 10698-1-AP), HAAO (Proteintech, cat: 12791-1-AP), Cleaved Caspase3 (CST, cat:9315), PARP (Proteintech, cat: 13371-1-AP), DUSP6 (Proteintech, cat: 10433-1-AP), IGFBP1 (Proteintech, cat: 13981-1-AP), p-ERK (CST, cat: 4370), ERK (CST, cat: 4695), BAD (CST, cat: 9239), BCL2 (CST, cat: 4223), BCL-XL (CST, cat: 2764), and β-actin (Santa Cruz, cat: 47778). .. The immunoblots were scanned using an Odyssey infrared imaging system (LI-COR).
Article Title: Kynurenine derivative 3-HAA is an agonist ligand for transcription factor YY1
Article Snippet: .. Antibodies against the following proteins were used for immunoblotting: IDO1 (CST, cat: 86630), TDO2 (Origene, cat: TA504879), KYNU (Proteintech, cat: 11796-1-AP), KMO (Proteintech, cat: 10698-1-AP), HAAO (Proteintech, cat: 12791-1-AP), Cleaved Caspase3 (CST, cat:9315), PARP (Proteintech, cat: 13371-1-AP), DUSP6 (Proteintech, cat: 10433-1-AP), IGFBP1 (Proteintech, cat: 13981-1-AP), p-ERK (CST, cat: 4370), ERK (CST, cat: 4695), BAD (CST, cat: 9239), BCL2 (CST, cat: 4223), BCL-XL (CST, cat: 2764), YY1 (Active Motif, cat: D110004), PKCζ (CST, cat: 9368), RIP3 (Proteintech, cat: 17563-1-AP), P62 (CST, cat: 5114), LC3B (CST, cat: 2775), CYP1A1 (Proteintech, cat: 13241-1-AP), CYP1B1 (Proteintech, cat: 18505-1-AP), TIPARP (Sigma, cat:SAB2102431) and β-actin (Santa Cruz, cat: 47778). .. The immunoblots were scanned using an Odyssey infrared imaging system (LI-COR).
Immunohistochemistry:Article Title: 3-HAA Metabolic Pathway Regulates HCC Growth
Article Snippet: .. Immunohistochemistry Tissue samples were xed in 4% paraformaldehyde (PFA) and embedded in para n. Primary antibodies used were 3-HAA (Abcam, ab15580), KYNU (Proteintech, cat: 11796-1-AP), KMO (Proteintech, cat: 10698- 1-AP), HAAO (Proteintech, cat: 12791-1-AP), IDO1 (CST, cat: 86630), and DUSP6 (Proteintech, cat: 10433- 1-AP). .. Detection was performed with the Elivision super Kit (MXB, cat: KIT-9921) and DAB Substrate (Boster, cat: AR1022), followed by hematoxylin counterstaining (BBI, cat: E607317).
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