Journal: Nature Communications
Article Title: Intranasal replicon vaccine establishes mucosal immunity and protects against H5N1 and H7N9 influenza
doi: 10.1038/s41467-025-64829-6
Figure Lengend Snippet: For all assays, n = 6 (3 male and 3 female) animals per group. A Animal survival after challenge with 1 × 10 6 pfu of intranasally-delivered A/Vietnam/1203/2004. B Averaged clinical scores. C Percent change in body weight from date of challenge. Two statistical hypotheses were tested within each figure, with filled lines showing comparisons to the vector control and dotted lines representing tests between key experimental groups. D – F Viral load in nasal wash at Day D 1, E 3, and F 5 post-challenge. Statistical analysis performed on log-transformed data using one-way ANOVA with Dunnett’s (filled lines) or Tukey’s (dotted lines) multiple comparisons test. G H5-binding IgG antibody titers in pre-challenge ferret serum. H H5 pseudovirus neutralization capacity (IC 50 ) of pre-challenge ferret serum. G Statistical analysis performed on log-transformed data used one-way ANOVA with Dunnett’s multiple comparisons test (filled lines and dotted lines). H Statistical analysis performed on log-transformed data using the Kruskal–Wallis test with Dunn’s multiple comparisons. Data are presented as D – G mean +/− SD or H geometric mean +/− geometric SD. IN Intranasal, IM Intramuscular, LLOD lower limit of detection, ns not significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Source data are provided as a Source Data file.
Article Snippet: An anti-HA A/Vietnam/1203/04 influenza virus (VN04-8) antibody (Rockland Immunochemicals #200-301-976) was used as a positive control for H5-specific ELISAs.
Techniques: Plasmid Preparation, Control, Transformation Assay, Binding Assay, Neutralization
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