pparα activator gw7647  (MedChemExpress)

Journal: Journal of Lipid Research

Article Title: PPARɑ variant V227A reduces plasma triglycerides through enhanced lipoprotein lipolysis

doi: 10.1016/j.jlr.2025.100806

Figure Lengend Snippet: V227A increases Lpl transcript abundance in liver. A: Genes that account for GSEA enrichment of lipoprotein catabolism gene set used in I. B: qRT-PCR of PPARɑ target genes using liver tissue from 6-h fasted chow-fed male (n = 11–15 per group), chow-fed female (n = 12 per group), Western diet-fed male (n = 10–11 per group), and Western diet-fed female mice (n = 6–8 per group). C: Lpl transcript abundance in liver tissue from male mice with indicated fasting duration as assessed by qRT-PCR (n = 4–9 per group). D: Gene expression in liver tissue from 6-h fasted male mice gavaged with PPARɑ agonist GW7647 (10 mg/kg) as assessed by qRT-PCR (n = 8). E: Ppara binding to Lpl enhancer (Enh) and promoter genomic regions in 6-h fasted V227A liver tissue (n = 5) as assessed by ChIP-qPCR. Enrichment is normalized to Rpl35 promoter region that does not exhibit known PPARɑ binding . ∗ = P < 0.05, ∗∗ = P < 0.01, ∗∗∗ = P < 0.001 as assessed by Student’s t test. Error bars are SEM.

Article Snippet: GW7647 (Cayman) was prepared in 0.5% hypromellose and gavaged at 16 and 21 h prior to tissue harvest.

Techniques: Quantitative RT-PCR, Western Blot, Gene Expression, Binding Assay, ChIP-qPCR