gst–cpsf2  (Abnova)

Journal: BMC Medicine

Article Title: Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke

doi: 10.1186/s12916-021-02001-9

Figure Lengend Snippet: List of antibody biomarkers for atherosclerosis

Article Snippet: GST–FOXJ2 and GST–CPSF2 were purchased from Abnova (Taipei, Taiwan).

Techniques: Protein Array, Membrane, Binding Assay, Activation Assay

Journal: BMC Medicine

Article Title: Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke

doi: 10.1186/s12916-021-02001-9

Figure Lengend Snippet: Presence of antibodies against DIDO1, FOXJ2, and CPSF2 in sera from a healthy donor (an HD) or a patient with transient ischemic attack (TIA) or acute ischemic stroke (AIS). Purified proteins of glutathione S-transferase (GST) (lane 1), GST–DIDO1 (1-275) (lane 2), GST–DIDO1 (271-545) (lane 3), GST–CPSF2 (lane 4), and GST–FOXJ2 (lane 5) were separated through sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by western blotting analysis using anti-GST ( b ), anti-DIDO1 ( c ), anti-FOXJ2 ( d ), anti-CPSF2 ( e ), and sera from HD-#30017 ( f ), patients with AIS-#07684 ( g ), AIS-#07581 ( i ), and AIS-#07115 ( k ), and those with TIA-#07207 ( h ), TIA-#07175 ( j ), and TIA-#07060 ( l ). The Coomassie brilliant blue (CBB) staining profile is also shown in a . M, molecular weight marker

Article Snippet: GST–FOXJ2 and GST–CPSF2 were purchased from Abnova (Taipei, Taiwan).

Techniques: Purification, Polyacrylamide Gel Electrophoresis, Western Blot, Staining, Molecular Weight, Marker

Journal: BMC Medicine

Article Title: Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke

doi: 10.1186/s12916-021-02001-9

Figure Lengend Snippet: Comparison of serum DIDO1-, CPSF2-, and FOXJ2-Ab levels between HDs and patients with TIA or AIS. GST-DIDO1:1-275 ( a ), biotinylated FOXJ2 peptide (bFOXJ2-426) ( d ), and biotinylated CPSF2 peptide (bCPSF2-607) ( g ) were used as the antigens. The AlphaLISA-determined serum antibody levels after subtraction of the levels against those of control GST are shown as box-whisker plots displaying the 10th, 20th, 50th, 80th, and 90th percentiles. P values were calculated by the Kruskal–Wallis test. * P < 0.05, ** P < 0.01, *** P < 0.001. The serum numbers of HDs, TIA, and AIS were 285, 92, and 464, respectively. The other information including total (male/female) numbers, average values, SDs, cutoff values, positive numbers, positive rates (%), and P values is summarized and shown in Table . Receiver operating characteristic curve (ROC) analysis was performed to assess the ability of DIDO1-Abs ( b , c ), FOXJ2-Abs ( e , f ), and CPSF2-Abs ( h , i ) to detect TIA and AIS. The numbers in the figures indicate the cutoff values for marker levels, and the numbers in parentheses indicate the sensitivity (left) and specificity (right). The areas under the curve (AUC), and 95% confidence intervals (CIs) are also shown in Table

Article Snippet: GST–FOXJ2 and GST–CPSF2 were purchased from Abnova (Taipei, Taiwan).

Techniques: Comparison, Control, Whisker Assay, Marker

Journal: BMC Medicine

Article Title: Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke

doi: 10.1186/s12916-021-02001-9

Figure Lengend Snippet: Comparison of serum DIDO1-, FOXJ2-, and CPSF2-Ab levels between HDs and patients with acute myocardial infarction (AMI) or diabetes mellitus (DM) examined by AlphaLISA

Article Snippet: GST–FOXJ2 and GST–CPSF2 were purchased from Abnova (Taipei, Taiwan).

Techniques: Comparison

Journal: BMC Medicine

Article Title: Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke

doi: 10.1186/s12916-021-02001-9

Figure Lengend Snippet: Receiver operating characteristic (ROC) analysis

Article Snippet: GST–FOXJ2 and GST–CPSF2 were purchased from Abnova (Taipei, Taiwan).

Techniques:

Journal: BMC Medicine

Article Title: Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke

doi: 10.1186/s12916-021-02001-9

Figure Lengend Snippet: Comparison of serum DIDO1-Abs, FOXJ2-Abs, and CPSF2-Abs levels between HDs and patients with acute myocardial infarction (AMI) and diabetes mellitus (DM). GST-DIDO1:1-275 ( a ), bFOXJ2-426 ( d ), and bCPSF2-607 ( g ) were used as antigens. Serum antibody levels in HDs and patients with AMI and type 2 DM were determined using AlphaLISA and are shown as box-whisker plots, as described in the legend of Fig. . The same results are summarized in Table . Responses to DIDO1-Abs ( b , c ), FOXJ2-Abs ( e , f ), and CPSF2-Abs ( h , i ) were also evaluated using ROC analysis, and summarized in Table

Article Snippet: GST–FOXJ2 and GST–CPSF2 were purchased from Abnova (Taipei, Taiwan).

Techniques: Comparison, Whisker Assay

Journal: BMC Medicine

Article Title: Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke

doi: 10.1186/s12916-021-02001-9

Figure Lengend Snippet: Comparison of the serum antibody levels of HDs versus those of patients with transient ischemic attack (TIA) or acute ischemic stroke (AIS)

Article Snippet: GST–FOXJ2 and GST–CPSF2 were purchased from Abnova (Taipei, Taiwan).

Techniques: Comparison

Journal: BMC Medicine

Article Title: Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke

doi: 10.1186/s12916-021-02001-9

Figure Lengend Snippet: Comparison of serum antibody levels of HDs versus those of patients with chronic kidney disease (CKD)

Article Snippet: GST–FOXJ2 and GST–CPSF2 were purchased from Abnova (Taipei, Taiwan).

Techniques: Comparison

Journal: BMC Medicine

Article Title: Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke

doi: 10.1186/s12916-021-02001-9

Figure Lengend Snippet: Comparison of serum DIDO1-Abs, FOXJ2-Abs, and CPSF2-Abs levels between HDs and patients with chronic kidney disease (CKD). Serum antibody levels against GST-DIDO1:1-275 protein ( a ), bFOXJ2-426 peptide ( e ), and bCPSF2-607 peptide ( i ) were compared between HDs and patients with CKD types 1, 2, and 3. The P values of CKD types 1, 2, and 3 versus HD controls are shown. Results are presented as described in the legend of Fig. . P values versus HD specimens are shown. The details are shown in Table . Responses to DIDO1-Abs ( b – d ), FOXJ2-Abs ( f – h ), and CPSF2-Abs ( j – l ) were also evaluated using the ROC analysis and are summarized in Table

Article Snippet: GST–FOXJ2 and GST–CPSF2 were purchased from Abnova (Taipei, Taiwan).

Techniques: Comparison

Journal: BMC Medicine

Article Title: Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke

doi: 10.1186/s12916-021-02001-9

Figure Lengend Snippet: Correlation analysis of antibody levels against synthetic bDIDO1, bCPSF2, and bFOXJ2 peptides with data of subjects in the Sawara Hospital cohort

Article Snippet: GST–FOXJ2 and GST–CPSF2 were purchased from Abnova (Taipei, Taiwan).

Techniques:

Journal: BMC Medicine

Article Title: Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke

doi: 10.1186/s12916-021-02001-9

Figure Lengend Snippet: Immunohistochemical staining of antigenic marker proteins in the atherosclerotic lesions. Surgically resected carotid atherosclerotic plaques were stained using immunohistochemistry. The antibodies used were anti-DIDO1 (Aviva Systems Biology), anti-FOXJ2 (Thermo Fisher Scientific), anti-CPSF2 (GeneTex), and anti-DHPS (Proteintech) antibodies for comparison. The tissue was also stained with antibodies against smooth muscle cell marker, vimentin (VIM) and smooth muscle actin (SMA), vascular endothelial cell marker, CD31 and CD34, and macrophage marker, CD68

Article Snippet: GST–FOXJ2 and GST–CPSF2 were purchased from Abnova (Taipei, Taiwan).

Techniques: Immunohistochemical staining, Staining, Marker, Immunohistochemistry, Comparison

Journal: BMC Medicine

Article Title: Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke

doi: 10.1186/s12916-021-02001-9

Figure Lengend Snippet: Results of the Japan Public Health Center (JPHC) cohort samples

Article Snippet: GST–FOXJ2 and GST–CPSF2 were purchased from Abnova (Taipei, Taiwan).

Techniques: