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gsk429286 (Tocris)

Name: gsk429286
Brand: Tocris
Rating: 94 (25 reviews)

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Tocris gsk429286

(A) Sensitivity indices for 5 dpf cyfip2 sibling (+/) and mutant (-/-) larvae, following a 40-minute heatshock at 120 hpf (5 dpf) to express either wildtype (Tg+; green), Rac1-(ΔRac1+; blue) or FMRP/eIF4E-(ΔFMRP+; pink) binding deficient versions of Cyfip2-EGFP. Comparisons were made to non-transgenic (Tg-), heatshocked sibling (+/) and mutant (-/-) controls. All indices (mean ± SD) compared using a Kruskal-Wallis test with Dunn’s multiple comparisons correction; p-values listed; p** < 0.01, p**** < 0.0001. (B) Sensitivity indices for 5 dpf cyfip2 wildtype (+/+; white bar) and heterozygous (+/-; gray bar) larvae, treated for 30 minutes on d5 with 5, 20 or 50 µM CK-869. Comparisons were made both within genotype and within condition. All indices (mean ± SD) compared using a Kruskal-Wallis test with Dunn’s multiple comparisons correction; p* < 0.05; p**** < 0.0001. (C) Sensitivity indices for 5 dpf Tüpfel longfin (TL) larvae treated for 30 minutes on d5 with the highest, non-lethal doses the formin antagonist (SMIFH2; 5 µM), PAK3 antagonist (IPA-3; 50 µM) and ROCK antagonist <t>(GSK429286;</t> 100 µM). Comparisons were made between respective treatments and the DMSO controls. All indices (mean ± SD) were compared using a Kruskal-Wallis test with Dunn’s multiple comparisons correction; All comparisons made were non-significant (n.s.). (D) Sensitivity indices for 5 dpf cyfip2 sibling (+/) and mutant (-/-) larvae, treated for 30 minutes on d5 with 100 or 500 µM MK-801. Comparisons were made both between genotypes within condition and between conditions by genotype. All indices (mean ± SD) were compared using an Ordinary one-way ANOVA with Tukey’s multiple comparisons correction. p** < 0.01; p*** < 0.001; p**** < 0.0001.

Gsk429286, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gsk429286/product/Tocris
Average 94 stars, based on 25 article reviews

gsk429286 - by Bioz Stars, 2026-02

94/100 stars

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1) Product Images from "Cyfip2 controls the acoustic startle threshold through FMRP, actin polymerization, and GABA B receptor function"

Article Title: Cyfip2 controls the acoustic startle threshold through FMRP, actin polymerization, and GABA B receptor function

Journal: bioRxiv

doi: 10.1101/2023.12.22.573054

Figure Legend Snippet: (A) Sensitivity indices for 5 dpf cyfip2 sibling (+/) and mutant (-/-) larvae, following a 40-minute heatshock at 120 hpf (5 dpf) to express either wildtype (Tg+; green), Rac1-(ΔRac1+; blue) or FMRP/eIF4E-(ΔFMRP+; pink) binding deficient versions of Cyfip2-EGFP. Comparisons were made to non-transgenic (Tg-), heatshocked sibling (+/) and mutant (-/-) controls. All indices (mean ± SD) compared using a Kruskal-Wallis test with Dunn’s multiple comparisons correction; p-values listed; p** < 0.01, p**** < 0.0001. (B) Sensitivity indices for 5 dpf cyfip2 wildtype (+/+; white bar) and heterozygous (+/-; gray bar) larvae, treated for 30 minutes on d5 with 5, 20 or 50 µM CK-869. Comparisons were made both within genotype and within condition. All indices (mean ± SD) compared using a Kruskal-Wallis test with Dunn’s multiple comparisons correction; p* < 0.05; p**** < 0.0001. (C) Sensitivity indices for 5 dpf Tüpfel longfin (TL) larvae treated for 30 minutes on d5 with the highest, non-lethal doses the formin antagonist (SMIFH2; 5 µM), PAK3 antagonist (IPA-3; 50 µM) and ROCK antagonist (GSK429286; 100 µM). Comparisons were made between respective treatments and the DMSO controls. All indices (mean ± SD) were compared using a Kruskal-Wallis test with Dunn’s multiple comparisons correction; All comparisons made were non-significant (n.s.). (D) Sensitivity indices for 5 dpf cyfip2 sibling (+/) and mutant (-/-) larvae, treated for 30 minutes on d5 with 100 or 500 µM MK-801. Comparisons were made both between genotypes within condition and between conditions by genotype. All indices (mean ± SD) were compared using an Ordinary one-way ANOVA with Tukey’s multiple comparisons correction. p** < 0.01; p*** < 0.001; p**** < 0.0001.

Techniques Used: Mutagenesis, Binding Assay, Transgenic Assay

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Effect of GSK429286 A on specific airway resistance (sRAW) in vivo conditions in experimental model of allergic asthma in guinea pigs. The measurement of the change’s values in sRaw after inhalation of 0.1 mM histamine for 30s after 3 weeks and 4 weeks inhalation of OVA. OVA – group represents guinea pigs sensitized with saline for 28 days and OVA + with ovalbumin. Administration of inhibitor of Rho-kinase, GSK429286 A, in concentration of 1 mg/kg (Rho-inh. 1) and 10 mg/kg (Rho-inh. 10) per os was carried out on the 15 th day of sensitization. Data are shown as average ± SEM; *p<0,0.05 OVA + versus tested substance.

Journal: International Journal of Immunopathology and Pharmacology

Article Title: Anti-astmatic effect of ROCK inhibitor, GSK429286 A, in experimentally induced allergic airway inflammation

doi: 10.1177/03946320241282949

Figure Lengend Snippet: Effect of GSK429286 A on specific airway resistance (sRAW) in vivo conditions in experimental model of allergic asthma in guinea pigs. The measurement of the change’s values in sRaw after inhalation of 0.1 mM histamine for 30s after 3 weeks and 4 weeks inhalation of OVA. OVA – group represents guinea pigs sensitized with saline for 28 days and OVA + with ovalbumin. Administration of inhibitor of Rho-kinase, GSK429286 A, in concentration of 1 mg/kg (Rho-inh. 1) and 10 mg/kg (Rho-inh. 10) per os was carried out on the 15 th day of sensitization. Data are shown as average ± SEM; *p<0,0.05 OVA + versus tested substance.

Article Snippet: Tested Rho-kinase inhibitor GSK429286 A (Rho-inh.) was obtained from MedChemExpress (MedChemExpress, New Jersey, USA).

Techniques: In Vivo, Saline, Concentration Assay

Effect of GSK429286 A on cough reflex response (A) and on ciliary beating frequency (CBF, B) in experimental model of allergic asthma in guinea pigs. The number of coughs after 3 min inhalation of 0.3 M citric acid solution was measured after 3 weeks and 4 weeks inhalation of OVA (A). Ciliary beating frequency was measured immediately after killing of animals (B). OVA – group represents guinea pigs sensitized with saline for 28 days and OVA + with ovalbumin. Administration of inhibitor of Rho-kinase, GSK429286 A, in concentration of 1 mg/kg (Rho-inh. 1) and 10 mg/kg (Rho-inh. 10) per os was carried out on the 15 th day of sensitization. Data are shown as average ± SEM; * p <0.05 and ** p <0.01 OVA + versus tested substance.

Journal: International Journal of Immunopathology and Pharmacology

Article Title: Anti-astmatic effect of ROCK inhibitor, GSK429286 A, in experimentally induced allergic airway inflammation

doi: 10.1177/03946320241282949

Figure Lengend Snippet: Effect of GSK429286 A on cough reflex response (A) and on ciliary beating frequency (CBF, B) in experimental model of allergic asthma in guinea pigs. The number of coughs after 3 min inhalation of 0.3 M citric acid solution was measured after 3 weeks and 4 weeks inhalation of OVA (A). Ciliary beating frequency was measured immediately after killing of animals (B). OVA – group represents guinea pigs sensitized with saline for 28 days and OVA + with ovalbumin. Administration of inhibitor of Rho-kinase, GSK429286 A, in concentration of 1 mg/kg (Rho-inh. 1) and 10 mg/kg (Rho-inh. 10) per os was carried out on the 15 th day of sensitization. Data are shown as average ± SEM; * p <0.05 and ** p <0.01 OVA + versus tested substance.

Article Snippet: Tested Rho-kinase inhibitor GSK429286 A (Rho-inh.) was obtained from MedChemExpress (MedChemExpress, New Jersey, USA).

Techniques: Saline, Concentration Assay

The concentration of nuclear factor kappa b (NF-κB) in lung tissue homogenates. The concentration of transcription factor NF-κB was measured by ELISA and subsequently quantified for the appropriate amount of proteins. OVA – group represents unsensitized guinea pigs treated with saline for 28 days and OVA + with ovalbumin. Administration of inhibitor of Rho-kinase, GSK429286 A, in concentration of 1 mg/kg (Rho-inh. 1) and 10 mg/kg (Rho-inh. 10) per os was carried out on the 15 th day of sensitization. Data are shown as average ± SEM; * p <0.05 and OVA + versus OVA-, and versus tested substance.

Journal: International Journal of Immunopathology and Pharmacology

Article Title: Anti-astmatic effect of ROCK inhibitor, GSK429286 A, in experimentally induced allergic airway inflammation

doi: 10.1177/03946320241282949

Figure Lengend Snippet: The concentration of nuclear factor kappa b (NF-κB) in lung tissue homogenates. The concentration of transcription factor NF-κB was measured by ELISA and subsequently quantified for the appropriate amount of proteins. OVA – group represents unsensitized guinea pigs treated with saline for 28 days and OVA + with ovalbumin. Administration of inhibitor of Rho-kinase, GSK429286 A, in concentration of 1 mg/kg (Rho-inh. 1) and 10 mg/kg (Rho-inh. 10) per os was carried out on the 15 th day of sensitization. Data are shown as average ± SEM; * p <0.05 and OVA + versus OVA-, and versus tested substance.

Article Snippet: Tested Rho-kinase inhibitor GSK429286 A (Rho-inh.) was obtained from MedChemExpress (MedChemExpress, New Jersey, USA).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Saline

The concentration of cytokines, namely IL-2, IL-4, IL-5 and IL-13 in lung tissue homogenates. The concentration of cytokines was measured by ELISA and subsequently quantified for the appropriate amount of proteins. OVA – group represents unsensitized guinea pigs treated with saline for 28 days and OVA + with ovalbumin. Administration of inhibitor of Rho-kinase, GSK429286 A, in concentration of 1 mg/kg (Rho-inh. 1) and 10 mg/kg (Rho-inh. 10) per os was carried out on the 15 th day of sensitization. Data are shown as average ± SEM; * p <0.05, *** p <0.001, **** p <0.0001 and OVA + versus OVA-, and versus tested substance.

Journal: International Journal of Immunopathology and Pharmacology

Article Title: Anti-astmatic effect of ROCK inhibitor, GSK429286 A, in experimentally induced allergic airway inflammation

doi: 10.1177/03946320241282949

Figure Lengend Snippet: The concentration of cytokines, namely IL-2, IL-4, IL-5 and IL-13 in lung tissue homogenates. The concentration of cytokines was measured by ELISA and subsequently quantified for the appropriate amount of proteins. OVA – group represents unsensitized guinea pigs treated with saline for 28 days and OVA + with ovalbumin. Administration of inhibitor of Rho-kinase, GSK429286 A, in concentration of 1 mg/kg (Rho-inh. 1) and 10 mg/kg (Rho-inh. 10) per os was carried out on the 15 th day of sensitization. Data are shown as average ± SEM; * p <0.05, *** p <0.001, **** p <0.0001 and OVA + versus OVA-, and versus tested substance.

Article Snippet: Tested Rho-kinase inhibitor GSK429286 A (Rho-inh.) was obtained from MedChemExpress (MedChemExpress, New Jersey, USA).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Saline

Immunohistochemical determination of the collagen I and III by Picrosirius red staining, amount of smooth muscle mass by antibodies against SMA, and amount of mucus by antibodies against MUC5AC in the lung section. The Picrosirius red staining, immunohistochemistry of SMA and MUC5AC was made on the lung section from guinea pigs sensibilized by saline (OVA -), ovalbumin (OVA +), and sensitized by ovalbumin and on the 15 th day of sensitization the administration of 10 mg/kg GSK429286 A (Rho-inh. 10) per os during 28 days of experiment. The amount of collagen in bronchioles was measured semi-quantitatively by using ZEN software. The signal from collagen from negative control (OVA-) serves as a 100% and all groups are compared to negative control (A). The amount of smooth muscle mass was measured by wall thickness in the bronchioles using Quick Photo Micro program (B), and the Image J program was used to determine mucus in the bronchioles (C). Data are shown as average ± SEM; ** p <0.01; **** p <0.0001 OVA + versus OVA – and versus tested substance.

Journal: International Journal of Immunopathology and Pharmacology

Article Title: Anti-astmatic effect of ROCK inhibitor, GSK429286 A, in experimentally induced allergic airway inflammation

doi: 10.1177/03946320241282949

Figure Lengend Snippet: Immunohistochemical determination of the collagen I and III by Picrosirius red staining, amount of smooth muscle mass by antibodies against SMA, and amount of mucus by antibodies against MUC5AC in the lung section. The Picrosirius red staining, immunohistochemistry of SMA and MUC5AC was made on the lung section from guinea pigs sensibilized by saline (OVA -), ovalbumin (OVA +), and sensitized by ovalbumin and on the 15 th day of sensitization the administration of 10 mg/kg GSK429286 A (Rho-inh. 10) per os during 28 days of experiment. The amount of collagen in bronchioles was measured semi-quantitatively by using ZEN software. The signal from collagen from negative control (OVA-) serves as a 100% and all groups are compared to negative control (A). The amount of smooth muscle mass was measured by wall thickness in the bronchioles using Quick Photo Micro program (B), and the Image J program was used to determine mucus in the bronchioles (C). Data are shown as average ± SEM; ** p <0.01; **** p <0.0001 OVA + versus OVA – and versus tested substance.

Article Snippet: Tested Rho-kinase inhibitor GSK429286 A (Rho-inh.) was obtained from MedChemExpress (MedChemExpress, New Jersey, USA).

Techniques: Immunohistochemical staining, Staining, Immunohistochemistry, Saline, Software, Negative Control

The changes of levels of collagen III (COL 3, A), collagen V (COL 5, B), transforming growth factor beta 1 (TGF-β1, C), and smooth muscle actin (SMA, D) in the lung homogenates. The concentration of COL one and 3, TGF-β1, and SMA proteins was measured by ELISA method and subsequently quantified for the appropriate amount of proteins. OVA – group represents unsensitized guinea pigs treated with saline for 28 days and OVA + with ovalbumin. Administration of inhibitor of Rho-kinase, GSK429286 A, in concentration of 1 mg/kg (Rho-inh. 1) and 10 mg/kg (Rho-inh. 10) per os was carried out on the 15 th day of sensitization. Data are shown as average ± SEM; * p <0.05 and ** p <0.01 OVA + versus OVA-, and versus tested substance.

Journal: International Journal of Immunopathology and Pharmacology

Article Title: Anti-astmatic effect of ROCK inhibitor, GSK429286 A, in experimentally induced allergic airway inflammation

doi: 10.1177/03946320241282949

Figure Lengend Snippet: The changes of levels of collagen III (COL 3, A), collagen V (COL 5, B), transforming growth factor beta 1 (TGF-β1, C), and smooth muscle actin (SMA, D) in the lung homogenates. The concentration of COL one and 3, TGF-β1, and SMA proteins was measured by ELISA method and subsequently quantified for the appropriate amount of proteins. OVA – group represents unsensitized guinea pigs treated with saline for 28 days and OVA + with ovalbumin. Administration of inhibitor of Rho-kinase, GSK429286 A, in concentration of 1 mg/kg (Rho-inh. 1) and 10 mg/kg (Rho-inh. 10) per os was carried out on the 15 th day of sensitization. Data are shown as average ± SEM; * p <0.05 and ** p <0.01 OVA + versus OVA-, and versus tested substance.

Article Snippet: Tested Rho-kinase inhibitor GSK429286 A (Rho-inh.) was obtained from MedChemExpress (MedChemExpress, New Jersey, USA).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Saline

Journal: bioRxiv

Article Title: Cyfip2 controls the acoustic startle threshold through FMRP, actin polymerization, and GABA B receptor function

doi: 10.1101/2023.12.22.573054

Figure Lengend Snippet: (A) Sensitivity indices for 5 dpf cyfip2 sibling (+/) and mutant (-/-) larvae, following a 40-minute heatshock at 120 hpf (5 dpf) to express either wildtype (Tg+; green), Rac1-(ΔRac1+; blue) or FMRP/eIF4E-(ΔFMRP+; pink) binding deficient versions of Cyfip2-EGFP. Comparisons were made to non-transgenic (Tg-), heatshocked sibling (+/) and mutant (-/-) controls. All indices (mean ± SD) compared using a Kruskal-Wallis test with Dunn’s multiple comparisons correction; p-values listed; p** < 0.01, p**** < 0.0001. (B) Sensitivity indices for 5 dpf cyfip2 wildtype (+/+; white bar) and heterozygous (+/-; gray bar) larvae, treated for 30 minutes on d5 with 5, 20 or 50 µM CK-869. Comparisons were made both within genotype and within condition. All indices (mean ± SD) compared using a Kruskal-Wallis test with Dunn’s multiple comparisons correction; p* < 0.05; p**** < 0.0001. (C) Sensitivity indices for 5 dpf Tüpfel longfin (TL) larvae treated for 30 minutes on d5 with the highest, non-lethal doses the formin antagonist (SMIFH2; 5 µM), PAK3 antagonist (IPA-3; 50 µM) and ROCK antagonist (GSK429286; 100 µM). Comparisons were made between respective treatments and the DMSO controls. All indices (mean ± SD) were compared using a Kruskal-Wallis test with Dunn’s multiple comparisons correction; All comparisons made were non-significant (n.s.). (D) Sensitivity indices for 5 dpf cyfip2 sibling (+/) and mutant (-/-) larvae, treated for 30 minutes on d5 with 100 or 500 µM MK-801. Comparisons were made both between genotypes within condition and between conditions by genotype. All indices (mean ± SD) were compared using an Ordinary one-way ANOVA with Tukey’s multiple comparisons correction. p** < 0.01; p*** < 0.001; p**** < 0.0001.

Article Snippet: SMIFH2, IPA-3, GSK429286 and NSC23766 were acquired from Tocris through Fisher Scientific.

Techniques: Mutagenesis, Binding Assay, Transgenic Assay

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1) Product Images from "Cyfip2 controls the acoustic startle threshold through FMRP, actin polymerization, and GABA B receptor function"

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