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Structured Review

Proteintech anti grb7
Anti Grb7, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/grb7/GRB7+Antibody/pm40691533-130-24-32
Average 93 stars, based on 9 article reviews
anti grb7 - by Bioz Stars, 2026-09
93/100 stars

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Related Articles

other:

Article Title: Novel nonphosphorylated peptides with conserved sequences selectively bind to Grb7 SH2 domain with affinity comparable to its phosphorylated ligand.
Article Snippet: Materials The ABL_1 and Grb2 plasmids were gifts from Professor Ruibao Ren (Brandeis University, USA), and the Grb7 (BC006535) and Grb14 cDNA (BC053559) plasmids were from the Proteintech Group.

Blocking Assay:

Article Title: Astragalus propinquus schischkin and Salvia miltiorrhiza bunge promote angiogenesis to treat myocardial ischemia via Ang-1/Tie-2/FAK pathway
Article Snippet: .. After blocking with 5% fat-free dry milk in Tris-buffered saline containing .1% Tween-20 for 2 h, the membranes were incubated with the following primary antibodies overnight at 4°C: Collagen 1 (1:1000, #72026, Cell Signaling), matrix metalloproteinase-9 (MMP9) (1:1000, 10375-2-AP, Proteintech), PDGFR-β (1:1000, 13449-1-ap, Proteintech), NG2 (1:1000, #4226, Cell Signaling), VE-cadherin (1:1000, ab33168, Abcam), VEGF (1:1000, 19003-1-ap, Proteintech), TGF-β (1:1000, 21898-1-ap, Proteintech), Ang-1 (1:1000, ab183701, Abcam), Ang-2 (1:1000, ab155106, Abcam), phospho-Tie-2 (1:1000, #4226, Cell Signaling), GRB7 (1:1000, 10045-1-Ig, Proteintech), FAK (1:1000, ab40794, Abcam) and β-actin (1:1000, 66009-1-lg, Proteintech). .. Then, the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies at room temperature for 1 h. The protein bands were visualized using an ECL Substrate (Thermo Fisher Scientific), and the quantification of the average densities of the protein bands was performed using the ImageJ software.

Saline:

Article Title: Astragalus propinquus schischkin and Salvia miltiorrhiza bunge promote angiogenesis to treat myocardial ischemia via Ang-1/Tie-2/FAK pathway
Article Snippet: .. After blocking with 5% fat-free dry milk in Tris-buffered saline containing .1% Tween-20 for 2 h, the membranes were incubated with the following primary antibodies overnight at 4°C: Collagen 1 (1:1000, #72026, Cell Signaling), matrix metalloproteinase-9 (MMP9) (1:1000, 10375-2-AP, Proteintech), PDGFR-β (1:1000, 13449-1-ap, Proteintech), NG2 (1:1000, #4226, Cell Signaling), VE-cadherin (1:1000, ab33168, Abcam), VEGF (1:1000, 19003-1-ap, Proteintech), TGF-β (1:1000, 21898-1-ap, Proteintech), Ang-1 (1:1000, ab183701, Abcam), Ang-2 (1:1000, ab155106, Abcam), phospho-Tie-2 (1:1000, #4226, Cell Signaling), GRB7 (1:1000, 10045-1-Ig, Proteintech), FAK (1:1000, ab40794, Abcam) and β-actin (1:1000, 66009-1-lg, Proteintech). .. Then, the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies at room temperature for 1 h. The protein bands were visualized using an ECL Substrate (Thermo Fisher Scientific), and the quantification of the average densities of the protein bands was performed using the ImageJ software.

Incubation:

Article Title: Astragalus propinquus schischkin and Salvia miltiorrhiza bunge promote angiogenesis to treat myocardial ischemia via Ang-1/Tie-2/FAK pathway
Article Snippet: .. After blocking with 5% fat-free dry milk in Tris-buffered saline containing .1% Tween-20 for 2 h, the membranes were incubated with the following primary antibodies overnight at 4°C: Collagen 1 (1:1000, #72026, Cell Signaling), matrix metalloproteinase-9 (MMP9) (1:1000, 10375-2-AP, Proteintech), PDGFR-β (1:1000, 13449-1-ap, Proteintech), NG2 (1:1000, #4226, Cell Signaling), VE-cadherin (1:1000, ab33168, Abcam), VEGF (1:1000, 19003-1-ap, Proteintech), TGF-β (1:1000, 21898-1-ap, Proteintech), Ang-1 (1:1000, ab183701, Abcam), Ang-2 (1:1000, ab155106, Abcam), phospho-Tie-2 (1:1000, #4226, Cell Signaling), GRB7 (1:1000, 10045-1-Ig, Proteintech), FAK (1:1000, ab40794, Abcam) and β-actin (1:1000, 66009-1-lg, Proteintech). .. Then, the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies at room temperature for 1 h. The protein bands were visualized using an ECL Substrate (Thermo Fisher Scientific), and the quantification of the average densities of the protein bands was performed using the ImageJ software.



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