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gramd1b  (Proteintech)


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    Proteintech gramd1b
    Figure 1. CRISPR/dCas9 activation of endogenous <t>GRAMD1B</t> gene expression in ARPE19 GRAMD1B-null cells. (A) ARPE-19 cells were transfected with scrambled DNA at 0.5, 1, and 1.5 µg DNA per well and GRAMD1B plasmid DNA at 0.5, 1, and 1.5 µg DNA per well, respectively. Western blot analysis was conducted with 50 µg of total protein per lane and showed that GRAMD1B is expressed before clonal selection and becomes enriched in post-clonal ARPE-19 cells (25 and 50 µg per well) treated with GRAMD1B plasmid DNA (1.5 µg per well). In contrast, GRAMD1B expression is absent in control cells treated with scrambled control DNA (1.5 µg per well). A549 cells were used as a positive control for GRAMD1B expression. β-Actin was used as a loading control. (B) Confocal imaging analysis with ARPE-19 cells transfected with plasmid DNA confirmed that GRAMD1B protein is expressed and mostly localized in the cytoplasm. Similar results were obtained from WB and ICC in more than three independent experiments. The scale bar is 10 µm.
    Gramd1b, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gramd1b/product/Proteintech
    Average 93 stars, based on 10 article reviews
    gramd1b - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "Aster-B Modulates Oxidative Stress Responses and Carotenoid Distribution in ARPE-19 Cells."

    Article Title: Aster-B Modulates Oxidative Stress Responses and Carotenoid Distribution in ARPE-19 Cells.

    Journal: Antioxidants (Basel, Switzerland)

    doi: 10.3390/antiox14050575

    Figure 1. CRISPR/dCas9 activation of endogenous GRAMD1B gene expression in ARPE19 GRAMD1B-null cells. (A) ARPE-19 cells were transfected with scrambled DNA at 0.5, 1, and 1.5 µg DNA per well and GRAMD1B plasmid DNA at 0.5, 1, and 1.5 µg DNA per well, respectively. Western blot analysis was conducted with 50 µg of total protein per lane and showed that GRAMD1B is expressed before clonal selection and becomes enriched in post-clonal ARPE-19 cells (25 and 50 µg per well) treated with GRAMD1B plasmid DNA (1.5 µg per well). In contrast, GRAMD1B expression is absent in control cells treated with scrambled control DNA (1.5 µg per well). A549 cells were used as a positive control for GRAMD1B expression. β-Actin was used as a loading control. (B) Confocal imaging analysis with ARPE-19 cells transfected with plasmid DNA confirmed that GRAMD1B protein is expressed and mostly localized in the cytoplasm. Similar results were obtained from WB and ICC in more than three independent experiments. The scale bar is 10 µm.
    Figure Legend Snippet: Figure 1. CRISPR/dCas9 activation of endogenous GRAMD1B gene expression in ARPE19 GRAMD1B-null cells. (A) ARPE-19 cells were transfected with scrambled DNA at 0.5, 1, and 1.5 µg DNA per well and GRAMD1B plasmid DNA at 0.5, 1, and 1.5 µg DNA per well, respectively. Western blot analysis was conducted with 50 µg of total protein per lane and showed that GRAMD1B is expressed before clonal selection and becomes enriched in post-clonal ARPE-19 cells (25 and 50 µg per well) treated with GRAMD1B plasmid DNA (1.5 µg per well). In contrast, GRAMD1B expression is absent in control cells treated with scrambled control DNA (1.5 µg per well). A549 cells were used as a positive control for GRAMD1B expression. β-Actin was used as a loading control. (B) Confocal imaging analysis with ARPE-19 cells transfected with plasmid DNA confirmed that GRAMD1B protein is expressed and mostly localized in the cytoplasm. Similar results were obtained from WB and ICC in more than three independent experiments. The scale bar is 10 µm.

    Techniques Used: CRISPR, Activation Assay, Gene Expression, Transfection, Plasmid Preparation, Western Blot, Selection, Expressing, Control, Positive Control, Imaging



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    Figure 1. CRISPR/dCas9 activation of endogenous <t>GRAMD1B</t> gene expression in ARPE19 GRAMD1B-null cells. (A) ARPE-19 cells were transfected with scrambled DNA at 0.5, 1, and 1.5 µg DNA per well and GRAMD1B plasmid DNA at 0.5, 1, and 1.5 µg DNA per well, respectively. Western blot analysis was conducted with 50 µg of total protein per lane and showed that GRAMD1B is expressed before clonal selection and becomes enriched in post-clonal ARPE-19 cells (25 and 50 µg per well) treated with GRAMD1B plasmid DNA (1.5 µg per well). In contrast, GRAMD1B expression is absent in control cells treated with scrambled control DNA (1.5 µg per well). A549 cells were used as a positive control for GRAMD1B expression. β-Actin was used as a loading control. (B) Confocal imaging analysis with ARPE-19 cells transfected with plasmid DNA confirmed that GRAMD1B protein is expressed and mostly localized in the cytoplasm. Similar results were obtained from WB and ICC in more than three independent experiments. The scale bar is 10 µm.
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    Figure 1. CRISPR/dCas9 activation of endogenous <t>GRAMD1B</t> gene expression in ARPE19 GRAMD1B-null cells. (A) ARPE-19 cells were transfected with scrambled DNA at 0.5, 1, and 1.5 µg DNA per well and GRAMD1B plasmid DNA at 0.5, 1, and 1.5 µg DNA per well, respectively. Western blot analysis was conducted with 50 µg of total protein per lane and showed that GRAMD1B is expressed before clonal selection and becomes enriched in post-clonal ARPE-19 cells (25 and 50 µg per well) treated with GRAMD1B plasmid DNA (1.5 µg per well). In contrast, GRAMD1B expression is absent in control cells treated with scrambled control DNA (1.5 µg per well). A549 cells were used as a positive control for GRAMD1B expression. β-Actin was used as a loading control. (B) Confocal imaging analysis with ARPE-19 cells transfected with plasmid DNA confirmed that GRAMD1B protein is expressed and mostly localized in the cytoplasm. Similar results were obtained from WB and ICC in more than three independent experiments. The scale bar is 10 µm.
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    CRISPR/dCas9 activation of endogenous <t>GRAMD1B</t> gene expression in ARPE19 GRAMD1B -null cells. ( A ) ARPE-19 cells were transfected with scrambled DNA at 0.5, 1, and 1.5 μg DNA per well and GRAMD1B plasmid DNA at 0.5, 1, and 1.5 μg DNA per well, respectively. Western blot analysis was conducted with 50 μg of total protein per lane and showed that GRAMD1B is expressed before clonal selection and becomes enriched in post-clonal ARPE-19 cells (25 and 50 μg per well) treated with GRAMD1B plasmid DNA (1.5 μg per well). In contrast, GRAMD1B expression is absent in control cells treated with scrambled control DNA (1.5 μg per well). A549 cells were used as a positive control for GRAMD1B expression. β-Actin was used as a loading control. ( B ) Confocal imaging analysis with ARPE-19 cells transfected with plasmid DNA confirmed that GRAMD1B protein is expressed and mostly localized in the cytoplasm. Similar results were obtained from WB and ICC in more than three independent experiments. The scale bar is 10 μm.
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    Image Search Results


    Figure 1. CRISPR/dCas9 activation of endogenous GRAMD1B gene expression in ARPE19 GRAMD1B-null cells. (A) ARPE-19 cells were transfected with scrambled DNA at 0.5, 1, and 1.5 µg DNA per well and GRAMD1B plasmid DNA at 0.5, 1, and 1.5 µg DNA per well, respectively. Western blot analysis was conducted with 50 µg of total protein per lane and showed that GRAMD1B is expressed before clonal selection and becomes enriched in post-clonal ARPE-19 cells (25 and 50 µg per well) treated with GRAMD1B plasmid DNA (1.5 µg per well). In contrast, GRAMD1B expression is absent in control cells treated with scrambled control DNA (1.5 µg per well). A549 cells were used as a positive control for GRAMD1B expression. β-Actin was used as a loading control. (B) Confocal imaging analysis with ARPE-19 cells transfected with plasmid DNA confirmed that GRAMD1B protein is expressed and mostly localized in the cytoplasm. Similar results were obtained from WB and ICC in more than three independent experiments. The scale bar is 10 µm.

    Journal: Antioxidants (Basel, Switzerland)

    Article Title: Aster-B Modulates Oxidative Stress Responses and Carotenoid Distribution in ARPE-19 Cells.

    doi: 10.3390/antiox14050575

    Figure Lengend Snippet: Figure 1. CRISPR/dCas9 activation of endogenous GRAMD1B gene expression in ARPE19 GRAMD1B-null cells. (A) ARPE-19 cells were transfected with scrambled DNA at 0.5, 1, and 1.5 µg DNA per well and GRAMD1B plasmid DNA at 0.5, 1, and 1.5 µg DNA per well, respectively. Western blot analysis was conducted with 50 µg of total protein per lane and showed that GRAMD1B is expressed before clonal selection and becomes enriched in post-clonal ARPE-19 cells (25 and 50 µg per well) treated with GRAMD1B plasmid DNA (1.5 µg per well). In contrast, GRAMD1B expression is absent in control cells treated with scrambled control DNA (1.5 µg per well). A549 cells were used as a positive control for GRAMD1B expression. β-Actin was used as a loading control. (B) Confocal imaging analysis with ARPE-19 cells transfected with plasmid DNA confirmed that GRAMD1B protein is expressed and mostly localized in the cytoplasm. Similar results were obtained from WB and ICC in more than three independent experiments. The scale bar is 10 µm.

    Article Snippet: The antibodies used included GRAMD1B (Proteintech), COXIV, Caspase-3, PARP, and Histone H3 (Cell Signaling Technology) at a dilution of 1:1000.

    Techniques: CRISPR, Activation Assay, Gene Expression, Transfection, Plasmid Preparation, Western Blot, Selection, Expressing, Control, Positive Control, Imaging

    Figure 1. CRISPR/dCas9 activation of endogenous GRAMD1B gene expression in ARPE19 GRAMD1B-null cells. (A) ARPE-19 cells were transfected with scrambled DNA at 0.5, 1, and 1.5 µg DNA per well and GRAMD1B plasmid DNA at 0.5, 1, and 1.5 µg DNA per well, respectively. Western blot analysis was conducted with 50 µg of total protein per lane and showed that GRAMD1B is expressed before clonal selection and becomes enriched in post-clonal ARPE-19 cells (25 and 50 µg per well) treated with GRAMD1B plasmid DNA (1.5 µg per well). In contrast, GRAMD1B expression is absent in control cells treated with scrambled control DNA (1.5 µg per well). A549 cells were used as a positive control for GRAMD1B expression. β-Actin was used as a loading control. (B) Confocal imaging analysis with ARPE-19 cells transfected with plasmid DNA confirmed that GRAMD1B protein is expressed and mostly localized in the cytoplasm. Similar results were obtained from WB and ICC in more than three independent experiments. The scale bar is 10 µm.

    Journal: Antioxidants (Basel, Switzerland)

    Article Title: Aster-B Modulates Oxidative Stress Responses and Carotenoid Distribution in ARPE-19 Cells.

    doi: 10.3390/antiox14050575

    Figure Lengend Snippet: Figure 1. CRISPR/dCas9 activation of endogenous GRAMD1B gene expression in ARPE19 GRAMD1B-null cells. (A) ARPE-19 cells were transfected with scrambled DNA at 0.5, 1, and 1.5 µg DNA per well and GRAMD1B plasmid DNA at 0.5, 1, and 1.5 µg DNA per well, respectively. Western blot analysis was conducted with 50 µg of total protein per lane and showed that GRAMD1B is expressed before clonal selection and becomes enriched in post-clonal ARPE-19 cells (25 and 50 µg per well) treated with GRAMD1B plasmid DNA (1.5 µg per well). In contrast, GRAMD1B expression is absent in control cells treated with scrambled control DNA (1.5 µg per well). A549 cells were used as a positive control for GRAMD1B expression. β-Actin was used as a loading control. (B) Confocal imaging analysis with ARPE-19 cells transfected with plasmid DNA confirmed that GRAMD1B protein is expressed and mostly localized in the cytoplasm. Similar results were obtained from WB and ICC in more than three independent experiments. The scale bar is 10 µm.

    Article Snippet: Control CRISPR Activation Plasmid (sc-437275), GRAMD1B CRISPR Activation Plasmid (h) (sc-408929-ACT), plasmid transfection medium (sc-108062), Ultra Cruz transfection reagent (sc-395739), hygromycin B (sc-29067), puromycin (sc-108071), and blasticidin S HCl (sc-204655A) (Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: CRISPR, Activation Assay, Gene Expression, Transfection, Plasmid Preparation, Western Blot, Selection, Expressing, Control, Positive Control, Imaging

    CRISPR/dCas9 activation of endogenous GRAMD1B gene expression in ARPE19 GRAMD1B -null cells. ( A ) ARPE-19 cells were transfected with scrambled DNA at 0.5, 1, and 1.5 μg DNA per well and GRAMD1B plasmid DNA at 0.5, 1, and 1.5 μg DNA per well, respectively. Western blot analysis was conducted with 50 μg of total protein per lane and showed that GRAMD1B is expressed before clonal selection and becomes enriched in post-clonal ARPE-19 cells (25 and 50 μg per well) treated with GRAMD1B plasmid DNA (1.5 μg per well). In contrast, GRAMD1B expression is absent in control cells treated with scrambled control DNA (1.5 μg per well). A549 cells were used as a positive control for GRAMD1B expression. β-Actin was used as a loading control. ( B ) Confocal imaging analysis with ARPE-19 cells transfected with plasmid DNA confirmed that GRAMD1B protein is expressed and mostly localized in the cytoplasm. Similar results were obtained from WB and ICC in more than three independent experiments. The scale bar is 10 μm.

    Journal: Antioxidants

    Article Title: Aster-B Modulates Oxidative Stress Responses and Carotenoid Distribution in ARPE-19 Cells

    doi: 10.3390/antiox14050575

    Figure Lengend Snippet: CRISPR/dCas9 activation of endogenous GRAMD1B gene expression in ARPE19 GRAMD1B -null cells. ( A ) ARPE-19 cells were transfected with scrambled DNA at 0.5, 1, and 1.5 μg DNA per well and GRAMD1B plasmid DNA at 0.5, 1, and 1.5 μg DNA per well, respectively. Western blot analysis was conducted with 50 μg of total protein per lane and showed that GRAMD1B is expressed before clonal selection and becomes enriched in post-clonal ARPE-19 cells (25 and 50 μg per well) treated with GRAMD1B plasmid DNA (1.5 μg per well). In contrast, GRAMD1B expression is absent in control cells treated with scrambled control DNA (1.5 μg per well). A549 cells were used as a positive control for GRAMD1B expression. β-Actin was used as a loading control. ( B ) Confocal imaging analysis with ARPE-19 cells transfected with plasmid DNA confirmed that GRAMD1B protein is expressed and mostly localized in the cytoplasm. Similar results were obtained from WB and ICC in more than three independent experiments. The scale bar is 10 μm.

    Article Snippet: Anti- GRAMD1B Polyclonal antibody (24905-1-AP, Proteintech, Rosemont, IL, USA), anti-Caspase-3 #9662, anti-NRF2 #20733S, anti-HO-1 #43966S, anti-P53 #9282 (Cell Signaling Technology, Danvers, MA, USA), Alexa Fluor 488-conjugated goat anti-rabbit IgG (A11008) HRP-conjugated goat anti-mouse and anti-rabbit IgG (H + L) (Invitrogen, Carlsbad, CA, USA), DAPI mounting medium (Southern biotech, Birmingham, AL, USA), HRP-conjugated secondary antibodies (Abcam, Waltham, MA, USA), Mitochondrial isolation kit, M-Per, Protease Inhibitor, Pierce ECL Western blotting substrate, BCA assay kit (Thermo Scientific, Waltham, MA, USA), PDVF membrane (Bio-Rad, Hercules, CA, USA), TaqMan primers (Thermo fisher, Waltham, MA, USA), TaqMan fast universal PCR master mix (Applied Biosystems, Foster City, CA, USA).

    Techniques: CRISPR, Activation Assay, Gene Expression, Transfection, Plasmid Preparation, Western Blot, Selection, Expressing, Control, Positive Control, Imaging