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gpr34 inhibitor  (MedChemExpress)


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    MedChemExpress gpr34 inhibitor
    Gpr34 Inhibitor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gpr34 inhibitor/product/MedChemExpress
    Average 94 stars, based on 3 article reviews
    gpr34 inhibitor - by Bioz Stars, 2026-02
    94/100 stars

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    A series of experiments to verify the expression of GPR34. (A) IHC was used to examine GPR34 expression in tumor tissues and normal tissues. Scale bars: 20 µm (n>3). (B) Western blotting analysis of GPR34 expression in MDA-MB-231 cells compared with MCF-10A cells. (C) RT-PCR analysis of GPR34 expression in MDA-MB-231 cells compared with MCF-10A cells (n=3). (D) Immunofluorescence staining for GPR34 and CD163 in human breast cancer tissue. Tissues were stained with DAPI. Blue: DPAI. Red: GPR34. The green represents CD163. Scale bars, 100 µm (n>3). IHC, immunohistochemistry; GPR34, G protein-coupled receptor 34; RT-PCR, reverse-transcriptase polymerase chain reaction; DAPI, 4',6-diamidino-2'-phenylindole. *, P<0.05.

    Journal: Translational Cancer Research

    Article Title: Integrated analysis reveals prognostic correlation and immune characteristics of a tumor-associated macrophage-based risk signature in triple-negative breast cancer

    doi: 10.21037/tcr-24-1037

    Figure Lengend Snippet: A series of experiments to verify the expression of GPR34. (A) IHC was used to examine GPR34 expression in tumor tissues and normal tissues. Scale bars: 20 µm (n>3). (B) Western blotting analysis of GPR34 expression in MDA-MB-231 cells compared with MCF-10A cells. (C) RT-PCR analysis of GPR34 expression in MDA-MB-231 cells compared with MCF-10A cells (n=3). (D) Immunofluorescence staining for GPR34 and CD163 in human breast cancer tissue. Tissues were stained with DAPI. Blue: DPAI. Red: GPR34. The green represents CD163. Scale bars, 100 µm (n>3). IHC, immunohistochemistry; GPR34, G protein-coupled receptor 34; RT-PCR, reverse-transcriptase polymerase chain reaction; DAPI, 4',6-diamidino-2'-phenylindole. *, P<0.05.

    Article Snippet: After being blocked in 5% nonfat dry milk, the membranes were then incubated at 4 °C in primary antibody against GPR34 (Abnova, PAB26304, Shanghai, China) overnight followed by incubation in HRP-conjugated secondary antibody (Beyotime, A0208, Shanghai, China) for 1 hour at room temperature.

    Techniques: Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Staining, Immunohistochemistry, Reverse Transcription, Polymerase Chain Reaction

    A series of experiments to verify the expression of GPR34. (A) IHC was used to examine GPR34 expression in tumor tissues and normal tissues. Scale bars: 20 µm (n>3). (B) Western blotting analysis of GPR34 expression in MDA-MB-231 cells compared with MCF-10A cells. (C) RT-PCR analysis of GPR34 expression in MDA-MB-231 cells compared with MCF-10A cells (n=3). (D) Immunofluorescence staining for GPR34 and CD163 in human breast cancer tissue. Tissues were stained with DAPI. Blue: DPAI. Red: GPR34. The green represents CD163. Scale bars, 100 µm (n>3). IHC, immunohistochemistry; GPR34, G protein-coupled receptor 34; RT-PCR, reverse-transcriptase polymerase chain reaction; DAPI, 4',6-diamidino-2'-phenylindole. *, P<0.05.

    Journal: Translational Cancer Research

    Article Title: Integrated analysis reveals prognostic correlation and immune characteristics of a tumor-associated macrophage-based risk signature in triple-negative breast cancer

    doi: 10.21037/tcr-24-1037

    Figure Lengend Snippet: A series of experiments to verify the expression of GPR34. (A) IHC was used to examine GPR34 expression in tumor tissues and normal tissues. Scale bars: 20 µm (n>3). (B) Western blotting analysis of GPR34 expression in MDA-MB-231 cells compared with MCF-10A cells. (C) RT-PCR analysis of GPR34 expression in MDA-MB-231 cells compared with MCF-10A cells (n=3). (D) Immunofluorescence staining for GPR34 and CD163 in human breast cancer tissue. Tissues were stained with DAPI. Blue: DPAI. Red: GPR34. The green represents CD163. Scale bars, 100 µm (n>3). IHC, immunohistochemistry; GPR34, G protein-coupled receptor 34; RT-PCR, reverse-transcriptase polymerase chain reaction; DAPI, 4',6-diamidino-2'-phenylindole. *, P<0.05.

    Article Snippet: After blocking the tissues, the primary antibody, GPR34 (Abnova, PAB26304), was applied at 1:200 dilution, overnight at 4 °C.

    Techniques: Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Staining, Immunohistochemistry, Reverse Transcription, Polymerase Chain Reaction