"Investigation of the association between the expression of plasmalogen biosynthesis and ferroptosis in COPD mice. ( A ) H&E staining of sections of mouse lung tissues from COPD model and normal group. ( B ) The concentration of IL-33 and IL-1α in the bronchoalveolar lavage fluid was determined by ELISA assay. ( C ) The neutrophils, macrophages, and lymphocytes in bronchoalveolar lavage fluid were quantified and compared between COPD and normal groups. ( D ) Tissue damage of lung were elevated by immunohistochemistry staingin <t>of</t> <t>Bax.</t> ( E ) GPX4 activity and the contents of GSH and MDA were analyzed between COPD and normal groups. ( F ) GPX4 expression in the lung tissues of mice was tested by immunohistochemistry (×100 and ×200). ( G ) The protein expression of GPX4, FAR-1, AGPS, and <t>GNPAT</t> was detected via western blot analysis. ( H ) The mRNA expression of FAR-1, AGPS, and GNPAT was measured via quantitative real-time PCR. ( I ) GNPAT expression in the lung tissues of mice was tested by immunohistochemistry (×100 and ×200). Data are presented as the mean ± SD of three replicates and analyzed using Student’s t-test. ns p > 0.05, ** p < 0.01, *** p < 0.001, compared with normal. The gels were cropped reasonably "