glut4  (Proteintech)

Journal: Genes & Diseases

Article Title: cTnIR193H restrictive cardiomyopathy mice satisfy high-energy metabolic demands through regulating glucose metabolism

doi: 10.1016/j.gendis.2025.101784

Figure Lengend Snippet: GLUT4 and CD36 expression levels. (A) GLUT4 and CD36 mRNA expression levels (WT, n = 4; cTnI193His-M, n = 3). (B) Immunohistochemical staining of GLUT4 and CD36 in heart tissue ( n = 3). Photographs were taken at 400× using an electron microscope. (C) CD36 and GLUT4 protein levels ( n = 3). (D) Statistical analysis of western blotting results for the detection of protein expression. (E) GLUT4 immunofluorescence staining on the cell membrane ( n = 3). (F) CD36 immunofluorescence staining on the cell membrane ( n = 3). Photographs were captured at 400× using a confocal laser. ∗ P < 0.05; ∗∗ P < 0.01.

Article Snippet: Western blotting analysis was used to detect the expression levels of GLUT4, CD36, PI3K, Akt, and p-Akt using specific antibodies (GLUT4, 1:2000, Proteintech, 66846-1-Ig, China; CD36, 1:500, Wanleibio, WL02390, China; PI3K, 1:1000, Wanleibio, WL03380, China; Akt, 1:500, Wanleibio, WL0003b, China; p-Akt, 1:500, Wanleibio, WLP001a, China).

Techniques: Expressing, Immunohistochemical staining, Staining, Microscopy, Western Blot, Immunofluorescence, Membrane

Journal: Genes & Diseases

Article Title: cTnIR193H restrictive cardiomyopathy mice satisfy high-energy metabolic demands through regulating glucose metabolism

doi: 10.1016/j.gendis.2025.101784

Figure Lengend Snippet: Cellular ATP concentration, glucose metabolism, and oxidative stress. (A) ATP concentration of primary cardiomyocytes 72 h after transfection of virus ( n = 3). (B) Glucose concentration in cell culture medium, 72 (Control, NC-AV, n = 4, cTnIR193H-AV, n = 3), 96 ( n = 5), and 108 ( n = 4) h after transfection of virus. (C) GLUT4 protein levels. (D) Statistical chart of GLUT4 protein expression levels ( n = 4). (E) PI3K protein levels. (F) Statistical chart of PI3K protein expression levels ( n = 5). (G) AKT and p-AKT protein levels. (H) Statistical chart of p-AKT/Akt protein expression ratio ( n = 4). (I) PI3K protein levels. (J) Statistical chart of PI3K protein expression levels ( n = 5). (K) AKT and p-AKT protein levels. (L) Statistical chart of p-AKT/Akt protein expression ratio ( n = 4). (M) Glucose concentration in cell culture medium, 72 ( n = 6), 96 ( n = 6), and 108 ( n = 5) h after transfection of virus. (N) Malondialdehyde (MDA) concentration ( n = 4) and superoxide dismutase (SOD) activity ( n = 3) in primary cardiomyocytes 130 h after transfection of virus. ∗ P < 0.05 versus control; ∗∗∗ P < 0.01 versus control; # P < 0.05 versus NC-AV; ## P < 0.01, ### P < 0.01, and #### P < 0.01 versus NC-AV; & P < 0.05 versus cTnIR193H-AV; && P < 0.01, &&& P < 0.01, and &&&& P < 0.01 versus cTnIR193H-AV.

Article Snippet: Western blotting analysis was used to detect the expression levels of GLUT4, CD36, PI3K, Akt, and p-Akt using specific antibodies (GLUT4, 1:2000, Proteintech, 66846-1-Ig, China; CD36, 1:500, Wanleibio, WL02390, China; PI3K, 1:1000, Wanleibio, WL03380, China; Akt, 1:500, Wanleibio, WL0003b, China; p-Akt, 1:500, Wanleibio, WLP001a, China).

Techniques: Concentration Assay, Transfection, Virus, Cell Culture, Control, Expressing, Activity Assay

Journal: Diabetes

Article Title: miR-432 Exacerbates Obesity-Induced Dysregulation of Glucose and Lipid Homeostasis

doi: 10.2337/db25-0295

Figure Lengend Snippet: cKI mice disrupt systemic metabolic homeostasis. A : Schematic of miR-432 knockin mouse construction. cKI mice were generated by crossing miR-432 ki/ki mice with adiponectin-cre, miR-432 ki/ki mice. Control (ctrl) mice were miR-432 ki/ki but lacking adiponectin-cre. B : Genotyping of WT, miR-432 ki/+ , miR-432 ki/ki (ctrl), and miR-432 ki/ki,cre (cKI) mice. C : Body weight of ctrl and cKI mice ( n = 8). D : Gross and adipose tissue appearance of ctrl and cKI mice. E : Tissue weight of ctrl and cKI mice ( n = 8). F : Adipose index of ctrl and cKI mice ([adipose mass / body mass] × 100) ( n = 8). G and H : Daily drinking and food intake measured via metabolic cages of ctrl and cKI mice over 24 h ( n = 6). I : H-E staining of epiWAT, iWAT, and BAT from ctrl and cKI mice (scale bar: 500 μm). J : Immunofluorescence of GLUT4 in epiWAT of ctrl and cKI mice (scale bar: 100 μm and 30 μm, respectively). L and M : Intraperitoneal GTT (2 g/kg glucose after 12 h of fasting) and ITT (0.5 units/kg premixed 30R after 4 h of fasting) ( n = 8) of ctrl and cKI mice. N – Q : Metabolic parameters of ctrl and cKI mice for 24 h (12-h light/12-h dark cycle): V o 2 , V co 2 , and heat production ( n = 6). Statistical analysis was performed using t test or nonparametric test, depending on data distribution. Two-way repeated-measures ANOVA was used to assess interaction effects between factors. * P < 0.05, ** P < 0.01. AUC, area under the curve; BGH pA, bovine growth hormone polyadenylation; visWAT, visceral white adipose tissue; WRPE, woodchuck hepatitis virus posttranscriptional regulatory element.

Article Snippet: For immunofluorescence, samples were incubated with GLUT4 antibody (Proteintech, Wuhan, China) at 4°C overnight, and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L) (Proteintech) for 60 min. Fluorescence was measured using confocal microscopy (Nikon, Tokyo, Japan).

Techniques: Knock-In, Generated, Control, Staining, Immunofluorescence, Virus