Journal: Pharmacology Research & Perspectives
Article Title: A novel and selective fluorescent ligand for the study of adenosine A 2B receptors
doi: 10.1002/prp2.1223
Figure Lengend Snippet: Effect of PSB603‐BY630 on Glosensor cAMP concentration‐response curves to the A 2B ‐selective agonist BAY 60–6583 in (A) HEK293G cells endogenously expressing A 2B R or (B) HEK293G cells overexpressing NLuc‐A 2B R. Concentration response curves were obtained in the absence and presence of 20 nM or 200 nM PSB603‐BY630. Values are mean ± S.E.M. of five separate experiments carried out in triplicate. Data represent peak luminescence response and are expressed as a percentage of the peak luminescence response to 3 μM BAY 60–6583 (in a) or 0.3 μM BAY 60–6583 (in b) obtained in the absence of antagonist in each individual experiment.
Article Snippet: A clonal HEK 293 cell line stably expressing the cAMP GloSensor (20F) biosensor (HEK293G) , was obtained from Promega Corporation (Madison, WI, USA).
Techniques: Concentration Assay, Expressing