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gelred  (Biotium)


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    Structured Review

    Biotium gelred
    Gelred, supplied by Biotium, used in various techniques. Bioz Stars score: 97/100, based on 3769 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gelred/GelRed+Nucleic+Acid+Gel+Stain/10__1016_slash_j__ijcha__2026__101938-104-12-13
    Average 97 stars, based on 3769 article reviews
    gelred - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: Bacillus subtilis ParB C-terminal lysine residues are essential for dimerization and in vivo function, indicating their roles in DNA sliding
    Article Snippet: During DNA–protein incubation, a 5% TBE (tris-borate-EDTA) precast gel (Bio-Rad, 4565015, Hercules, CA) in TBE buffer (89 mM Tris, pH 7.6, 89 mM boric acid, 2 mM EDTA) was pre-run at 120 V at 4°C for 30 min to remove ions from the gel. .. Upon completion of the pre-run, 17 μl of the protein–DNA mixture were loaded onto each gel well, and an electrophoresis was performed at 120 V at 4°C for 1 h. The gel was stained for 30 min in GelRed (Biotium, 41003, Fremont, CA) solution before imaging. ..

    Article Title: Bacillus subtilis ParB C-terminal lysine residues are essential for dimerization and in vivo function, indicating their roles in DNA sliding.
    Article Snippet: During DNA –protein incubation, a 5% TBE (tris-borate-EDTA) precast gel (Bio-Rad, 4565015, Hercules, CA) in TBE buffer (89 mM Tris, pH 7.6, 89 mM boric acid, 2 mM EDTA) was prerun at 120 V at 4 ◦C for 30 min to remove ions from the gel. .. Upon completion of the pre-run, 17 μl of the protein –DNA mixture were loaded onto each gel well, and an electrophoresis was performed at 120 V at 4 ◦C for 1 h. The gel was stained for 30 min in GelRed (Biotium, 41003, Fremont, CA) solution before imaging. ..

    Article Title: Fanconi Anemia in Mexican Patients: Molecular Spectrum and Clinical Manifestations in a Case Series.
    Article Snippet: Genomic DNA was isolated from PB using QIAamp® DNA Blood Mini kit (Qiagen GmbH, Germantown, MD, USA) according to the manufacturer’s instructions. .. Genomic isolated DNA integrity was analyzed by electrophoresis on 1% agarose gel stained with GelRed (Biotium, Fremont, CA, USA; Cat. No. 41003). .. DNA concentration was determined using spectrophotometry, with the absorbance (A) at 260 nm (A260) measured; genomic DNA “purity” was assessed by reading the A230 and A280, resulting in values of ~1.8 and 2.1 for A260/A280 and A260/A230 ratio, according to the results obtained with the NanoDropTM One (NanoDrop Technologies; Thermo Fisher Scientific, Inc., Grand Island, NY, USA).

    Article Title: Heterogeneity of estrogen and β-adrenergic receptors in female human coronary artery endothelial cells
    Article Snippet: .. A 4% agarose gel was prepared in 1x TBE buffer, stained with GelRed (Biotium, 41003), and electrophoresis with a GeneRuler 50 bp DNA Ladder (Thermo Fisher Scientific, SM0371) as a size marker. .. Electrophoresis was performed at 120 V for 1–2 h. Gel images were captured using the FUSION FX VILBER system.

    Staining:

    Article Title: Bacillus subtilis ParB C-terminal lysine residues are essential for dimerization and in vivo function, indicating their roles in DNA sliding
    Article Snippet: During DNA–protein incubation, a 5% TBE (tris-borate-EDTA) precast gel (Bio-Rad, 4565015, Hercules, CA) in TBE buffer (89 mM Tris, pH 7.6, 89 mM boric acid, 2 mM EDTA) was pre-run at 120 V at 4°C for 30 min to remove ions from the gel. .. Upon completion of the pre-run, 17 μl of the protein–DNA mixture were loaded onto each gel well, and an electrophoresis was performed at 120 V at 4°C for 1 h. The gel was stained for 30 min in GelRed (Biotium, 41003, Fremont, CA) solution before imaging. ..

    Article Title: Bacillus subtilis ParB C-terminal lysine residues are essential for dimerization and in vivo function, indicating their roles in DNA sliding.
    Article Snippet: During DNA –protein incubation, a 5% TBE (tris-borate-EDTA) precast gel (Bio-Rad, 4565015, Hercules, CA) in TBE buffer (89 mM Tris, pH 7.6, 89 mM boric acid, 2 mM EDTA) was prerun at 120 V at 4 ◦C for 30 min to remove ions from the gel. .. Upon completion of the pre-run, 17 μl of the protein –DNA mixture were loaded onto each gel well, and an electrophoresis was performed at 120 V at 4 ◦C for 1 h. The gel was stained for 30 min in GelRed (Biotium, 41003, Fremont, CA) solution before imaging. ..

    Article Title: Fanconi Anemia in Mexican Patients: Molecular Spectrum and Clinical Manifestations in a Case Series.
    Article Snippet: Genomic DNA was isolated from PB using QIAamp® DNA Blood Mini kit (Qiagen GmbH, Germantown, MD, USA) according to the manufacturer’s instructions. .. Genomic isolated DNA integrity was analyzed by electrophoresis on 1% agarose gel stained with GelRed (Biotium, Fremont, CA, USA; Cat. No. 41003). .. DNA concentration was determined using spectrophotometry, with the absorbance (A) at 260 nm (A260) measured; genomic DNA “purity” was assessed by reading the A230 and A280, resulting in values of ~1.8 and 2.1 for A260/A280 and A260/A230 ratio, according to the results obtained with the NanoDropTM One (NanoDrop Technologies; Thermo Fisher Scientific, Inc., Grand Island, NY, USA).

    Article Title: Heterogeneity of estrogen and β-adrenergic receptors in female human coronary artery endothelial cells
    Article Snippet: .. A 4% agarose gel was prepared in 1x TBE buffer, stained with GelRed (Biotium, 41003), and electrophoresis with a GeneRuler 50 bp DNA Ladder (Thermo Fisher Scientific, SM0371) as a size marker. .. Electrophoresis was performed at 120 V for 1–2 h. Gel images were captured using the FUSION FX VILBER system.

    Article Title: A Simple Tool for the Design of Polyhedral DNA Nanostructures
    Article Snippet: .. The gel was then stained with GelRed [Biotium, Cat. No. 41003], diluted to 3× in 0.1 M NaCl solution, for 30 minutes on a gel rocker [Benchmark Scientific, Cat. No. BR2000]. ..

    Imaging:

    Article Title: Bacillus subtilis ParB C-terminal lysine residues are essential for dimerization and in vivo function, indicating their roles in DNA sliding
    Article Snippet: During DNA–protein incubation, a 5% TBE (tris-borate-EDTA) precast gel (Bio-Rad, 4565015, Hercules, CA) in TBE buffer (89 mM Tris, pH 7.6, 89 mM boric acid, 2 mM EDTA) was pre-run at 120 V at 4°C for 30 min to remove ions from the gel. .. Upon completion of the pre-run, 17 μl of the protein–DNA mixture were loaded onto each gel well, and an electrophoresis was performed at 120 V at 4°C for 1 h. The gel was stained for 30 min in GelRed (Biotium, 41003, Fremont, CA) solution before imaging. ..

    Article Title: Bacillus subtilis ParB C-terminal lysine residues are essential for dimerization and in vivo function, indicating their roles in DNA sliding.
    Article Snippet: During DNA –protein incubation, a 5% TBE (tris-borate-EDTA) precast gel (Bio-Rad, 4565015, Hercules, CA) in TBE buffer (89 mM Tris, pH 7.6, 89 mM boric acid, 2 mM EDTA) was prerun at 120 V at 4 ◦C for 30 min to remove ions from the gel. .. Upon completion of the pre-run, 17 μl of the protein –DNA mixture were loaded onto each gel well, and an electrophoresis was performed at 120 V at 4 ◦C for 1 h. The gel was stained for 30 min in GelRed (Biotium, 41003, Fremont, CA) solution before imaging. ..

    Isolation:

    Article Title: Fanconi Anemia in Mexican Patients: Molecular Spectrum and Clinical Manifestations in a Case Series.
    Article Snippet: Genomic DNA was isolated from PB using QIAamp® DNA Blood Mini kit (Qiagen GmbH, Germantown, MD, USA) according to the manufacturer’s instructions. .. Genomic isolated DNA integrity was analyzed by electrophoresis on 1% agarose gel stained with GelRed (Biotium, Fremont, CA, USA; Cat. No. 41003). .. DNA concentration was determined using spectrophotometry, with the absorbance (A) at 260 nm (A260) measured; genomic DNA “purity” was assessed by reading the A230 and A280, resulting in values of ~1.8 and 2.1 for A260/A280 and A260/A230 ratio, according to the results obtained with the NanoDropTM One (NanoDrop Technologies; Thermo Fisher Scientific, Inc., Grand Island, NY, USA).

    Agarose Gel Electrophoresis:

    Article Title: Fanconi Anemia in Mexican Patients: Molecular Spectrum and Clinical Manifestations in a Case Series.
    Article Snippet: Genomic DNA was isolated from PB using QIAamp® DNA Blood Mini kit (Qiagen GmbH, Germantown, MD, USA) according to the manufacturer’s instructions. .. Genomic isolated DNA integrity was analyzed by electrophoresis on 1% agarose gel stained with GelRed (Biotium, Fremont, CA, USA; Cat. No. 41003). .. DNA concentration was determined using spectrophotometry, with the absorbance (A) at 260 nm (A260) measured; genomic DNA “purity” was assessed by reading the A230 and A280, resulting in values of ~1.8 and 2.1 for A260/A280 and A260/A230 ratio, according to the results obtained with the NanoDropTM One (NanoDrop Technologies; Thermo Fisher Scientific, Inc., Grand Island, NY, USA).

    Article Title: Automated plasmid purification system enables increased AAV production.
    Article Snippet: NanoDrop1000 (Thermo Scientific, Wilmington, DE, USA) was used to quantify potential plasmid contaminations (A260/A280 and A260/A230). .. To determine the level of supercoiling, 20 ng of each plasmid was loaded onto a 1% agarose gel with 1:10 000 v/v of GelRed (41003–1, Biotium, San Francisco, USA). .. The gel electrophoresis was run in 1x TAE buffer at 180 V in Sub-Cell GT cell (Bio-Rad) for 1 h and then imaged in Gel Doc EZ (Bio-Rad Laboratories, Hercules, CA, USA).

    Article Title: Heterogeneity of estrogen and β-adrenergic receptors in female human coronary artery endothelial cells
    Article Snippet: .. A 4% agarose gel was prepared in 1x TBE buffer, stained with GelRed (Biotium, 41003), and electrophoresis with a GeneRuler 50 bp DNA Ladder (Thermo Fisher Scientific, SM0371) as a size marker. .. Electrophoresis was performed at 120 V for 1–2 h. Gel images were captured using the FUSION FX VILBER system.

    Plasmid Preparation:

    Article Title: Automated plasmid purification system enables increased AAV production.
    Article Snippet: NanoDrop1000 (Thermo Scientific, Wilmington, DE, USA) was used to quantify potential plasmid contaminations (A260/A280 and A260/A230). .. To determine the level of supercoiling, 20 ng of each plasmid was loaded onto a 1% agarose gel with 1:10 000 v/v of GelRed (41003–1, Biotium, San Francisco, USA). .. The gel electrophoresis was run in 1x TAE buffer at 180 V in Sub-Cell GT cell (Bio-Rad) for 1 h and then imaged in Gel Doc EZ (Bio-Rad Laboratories, Hercules, CA, USA).

    Marker:

    Article Title: Heterogeneity of estrogen and β-adrenergic receptors in female human coronary artery endothelial cells
    Article Snippet: .. A 4% agarose gel was prepared in 1x TBE buffer, stained with GelRed (Biotium, 41003), and electrophoresis with a GeneRuler 50 bp DNA Ladder (Thermo Fisher Scientific, SM0371) as a size marker. .. Electrophoresis was performed at 120 V for 1–2 h. Gel images were captured using the FUSION FX VILBER system.



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