Journal: Nucleic Acids Research
Article Title: Extensive homologous recombination safeguards oocyte genome integrity in mammals
doi: 10.1093/nar/gkae1304
Figure Lengend Snippet: FIRRM-deleted oocytes accumulate nuclear RAD51 foci. ( A ) Schematic diagram showing the start points of CRE expression in Stra8-Cre , Gdf9-Cre and Zp3-Cre strains during ovarian development. E13.5, embryonic day 13.5. The relevant ovarian follicles are indicated. ( B ) Immunohistochemical staining of FIRRM in WT, Firrm zKO and Firrm gKO ovaries at PD21. The nuclei of oocytes are indicated with dashed lines. Scale bar, 50 μm. ( C ) Quantification of nuclear RAD51 intensity in oocytes of primordial follicles and primary follicles in WT, Firrm zKO and Firrm gKO ovaries. Error bars indicate SD. n indicates the number of oocytes analyzed. n.s., P > 0.05; *, P < 0.05 and ***, P < 0.001 (Mann-Whitney nonparametric test). ( D and E ) IF staining detects RAD51 signal in WT, Firrm zKO and Firrm gKO ovaries at PD5 and PD8. Representative images of primordial follicles (D) and primary follicles (E) are shown. Oocytes are indicated with dashed lines. Scale bar, 20 μm.
Article Snippet: Firrm flox , H11-CAG-CreERT2 and H11-Gdf9-iCre mice were purchased from the GemPharmatech Co., Ltd. Transgenic Zp3-Cre was reported previously ( ).
Techniques: Expressing, Immunohistochemical staining, Staining, MANN-WHITNEY