modifier subunit  (Proteintech)

Journal: Redox Biology

Article Title: The electrophilic metabolite of kynurenine, kynurenine-CKA, requires C151 in Keap1 to derepress Nrf2

doi: 10.1016/j.redox.2026.104009

Figure Lengend Snippet: Kynurenine induces Nrf2-driven transcription in BMDMs . WT and Nrf2-KO BMDMs were seeded into six-well plates (1 × 10 6 /well), allowed to adhere overnight, and then treated for 18 h with vehicle or kynurenine (Kyn; 20 μM or 200 μM). The levels of mRNA for Nrf2 (gene name Nfe2l2 ) (A) , Keap1 (B) , and the Nrf2 transcriptional targets Nqo1 (C) , Gclm (D) , Gclc (E) , Slc7a11 (F) , and Slc7a5 (G) were determined by quantitative RT-PCR after normalization to the housekeeping control Rps18 (or B2m for Keap1 ). Bars represent means ± SEM (n = 3 biologically independent replicates per condition), with individual data points shown. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001.

Article Snippet: The TaqManTM Gene Expression Assay IDs (Thermo Fisher, Waltham, MA) were: Mm01253561 (for Nqo1 ); Mm00516005 (for Ho1); Mm01324400 (for Gclm ); Mm00434228 (for IL1b ); Mm00446190 (for IL6 ); Mm00441242 (for Mcp1 ); Mm00443252 (for Tnf ); Mm004405021 (for Nos2 ); Mm00437762 (for B2m ); Mm03024075 (for Hprt1 ); and 4352341E (for Actb ).

Techniques: Quantitative RT-PCR, Control

Journal: Redox Biology

Article Title: The electrophilic metabolite of kynurenine, kynurenine-CKA, requires C151 in Keap1 to derepress Nrf2

doi: 10.1016/j.redox.2026.104009

Figure Lengend Snippet: The low-dose anti-inflammatory effects of Kyn-CKA in BMDMs are dependent on Nrf2. (A – B) Treatment with Kyn-CKA (5 h) fails to induce the expression of Nqo1 and Gclm in BMDMs obtained from Nrf2-knockout (Nrf2 −/− ) mice. Data are n = 3, ∗∗∗∗p < 0.0001 by two-way ANOVA and Tukey's post-test. (C – G) Effects of Kyn-CKA on the expression of NF-κB−regulated genes in WT and Nrf2-KO BMDMs following 5 h incubation with LPS (0.5 μg/mL) and the indicated concentrations of Kyn-CKA. Data are n = 6, ∗p < 0.05, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 by two-way ANOVA and Tukey's post-test. Nrf2 was identified as a significant source of variation for MCP1, IL6, TNFα, Nos2, (p < 0.0001) and IL1β (p < 0.005).

Article Snippet: The TaqManTM Gene Expression Assay IDs (Thermo Fisher, Waltham, MA) were: Mm01253561 (for Nqo1 ); Mm00516005 (for Ho1); Mm01324400 (for Gclm ); Mm00434228 (for IL1b ); Mm00446190 (for IL6 ); Mm00441242 (for Mcp1 ); Mm00443252 (for Tnf ); Mm004405021 (for Nos2 ); Mm00437762 (for B2m ); Mm03024075 (for Hprt1 ); and 4352341E (for Actb ).

Techniques: Expressing, Knock-Out, Incubation