Journal: mSphere
Article Title: The essential kinase TgGSK regulates centrosome segregation and endodyogeny in Toxoplasma gondii
doi: 10.1128/msphere.00111-25
Figure Lengend Snippet: Acetylation by GCN5b stabilizes TgGSK. (A) Plot of TgGSK immunoprecipitation data. More significant interactors are toward the upper right of the plot. Points in red are members of the GCN5b complex. (B) IFA of non-dividing GSK.3xHA parasites treated with 0, 2, or 4 µM garcinol for 18 hours. Staining was done for IMC3, HA, and DAPI to visualize IMC, TgGSK, and nuclear material, respectively. (C) Western blot analysis of TgGSK protein levels after 18 hours of garcinol treatment. The cytosolic protein aldolase was used as a control.
Article Snippet: GSK.3xHA parasites were seeded simultaneously with 2 or 4 μM Garcinol (BOC Sciences) in 1% serum media for 18 hours.
Techniques: Immunoprecipitation, Staining, Western Blot, Control