galactomannan antigen  (Bio-Rad)

Journal: bioRxiv

Article Title: Unusual molecular architecture of a human gut microbiota β-mannanase reveals a new CBM family

doi: 10.64898/2026.02.11.704390

Figure Lengend Snippet: A) Heatmap showing maximum OD 600 values reached by 19 human gut Bacteroides and Phocaeicola species when grown on 5 mg/ml β-mannan substrates – konjac glucomannan, guar gum galactomannan, and carob galactomannan. Bacteria were grown in 96-well plate (200 µl well volume) over a course of 48 h. The OD 600 values of negative controls were subtracted before plotting. B) Corresponding growth curves used to plot the heatmap.

Article Snippet: Mannooligosaccharides, cellooligosaccharides, carob galactomannan (low-viscosity), ivory nut mannan, konjac glucomannan (low-viscosity), wheat arabinoxylan, xyloglucan, barley and oat β-glucans were purchased from MegaZyme (Bray, Ireland).

Techniques: Guar Gum, Bacteria

Journal: bioRxiv

Article Title: Unusual molecular architecture of a human gut microbiota β-mannanase reveals a new CBM family

doi: 10.64898/2026.02.11.704390

Figure Lengend Snippet: Glycans used are carob galactomannan (CGM), mannopentaose (M5), cellopentaose (G5), and di-alpha-D-galactosyl-mannopentaose (GM5). Titrations were conducted at 25 °C in 20 mM Tris buffer, pH 8.0, containing 150 mM NaCl. Upper parts of each panel are raw binding heats, lower parts are integrated data fit to single set of sites model if binding observed.

Article Snippet: Mannooligosaccharides, cellooligosaccharides, carob galactomannan (low-viscosity), ivory nut mannan, konjac glucomannan (low-viscosity), wheat arabinoxylan, xyloglucan, barley and oat β-glucans were purchased from MegaZyme (Bray, Ireland).

Techniques: Binding Assay

Journal: Molecular Microbiology

Article Title: Late Stage Mannan Metabolism in Cellvibrio japonicus Requires the Combined Action of a Mannosyl‐Glucose Phosphorylase and a Mannobiose Epimerase

doi: 10.1111/mmi.70043

Figure Lengend Snippet: Growth analyses of C. japonicus strains grown in MOPS defined media supplemented with 0.5% (w/v) of (A) mannobiose, (B) mannotriose, (C) mannosyl‐glucose, (D) cellobiose, (E) glucomannan, (F) carob galactomannan, (G) guar galactomannan, or (H) linear mannan. Notably, panels A—D were grown on a separate microplate from panels E—F and are shown together for ease of visualization. Growth analyses were conducted using an EPOCH2 microplate reader (Biotek) in biological triplicate for 48 h. Error bars depict standard deviation but are too small to be observed in some graphs. Complete growth statistics are available in Table .

Article Snippet: Glucomannan (40 glucose: 60 mannose), carob galactomannan (21 galactose: 79 mannose), guar galactomannan (38 galactose: 62 mannose), and linear ivory nut mannan were obtained from Megazyme (cat. no. P‐GLCML, P‐GALML, P‐GGMMV, and P‐MANIV).

Techniques: Standard Deviation

Journal: Molecular Microbiology

Article Title: Late Stage Mannan Metabolism in Cellvibrio japonicus Requires the Combined Action of a Mannosyl‐Glucose Phosphorylase and a Mannobiose Epimerase

doi: 10.1111/mmi.70043

Figure Lengend Snippet: Growth analyses of C. japonicus single gene deletions, intermediate strains, and double ectopic complementation strains grown in MOPS defined media supplemented with 0.5% (w/v) of either (A) mannobiose, (B) mannotriose, (C) glucomannan, (D) carob galactomannan, (E) guar galactomannan, or (F) linear mannan. Panels A, B, and F were grown on a separate microplate from Panels C, D, and E, and are shown here together for ease of visualization. Growth analyses were conducted using an EPOCH2 microplate reader (Biotek) in biological triplicate for 48 h. Error bars depict standard deviation but are too small to be observed in many of the graphs. Complete growth statistics are available in Table .

Article Snippet: Glucomannan (40 glucose: 60 mannose), carob galactomannan (21 galactose: 79 mannose), guar galactomannan (38 galactose: 62 mannose), and linear ivory nut mannan were obtained from Megazyme (cat. no. P‐GLCML, P‐GALML, P‐GGMMV, and P‐MANIV).

Techniques: Standard Deviation

Journal: Molecular Microbiology

Article Title: Late Stage Mannan Metabolism in Cellvibrio japonicus Requires the Combined Action of a Mannosyl‐Glucose Phosphorylase and a Mannobiose Epimerase

doi: 10.1111/mmi.70043

Figure Lengend Snippet: Growth analyses of C. japonicus pJKN5 complement strains grown in MOPS defined media supplemented with 0.5% (w/v) of either (A) mannobiose, (B) mannotriose, (C) glucomannan, (D) carob galactomannan, (E) guar galactomannan, or (F) linear mannan. Notably, panels A, B, and F were grown on a separate microplate from panels C, D, and E and are shown together for ease of visualization. Growth analyses were conducted in an EPOCH2 microplate reader (Biotek) in biological triplicate for 48 h. Error bars depict standard deviation and complete growth statistics are available in Table .

Article Snippet: Glucomannan (40 glucose: 60 mannose), carob galactomannan (21 galactose: 79 mannose), guar galactomannan (38 galactose: 62 mannose), and linear ivory nut mannan were obtained from Megazyme (cat. no. P‐GLCML, P‐GALML, P‐GGMMV, and P‐MANIV).

Techniques: Standard Deviation