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anti β gal  (Proteintech)


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    Structured Review

    Proteintech anti β gal
    Anti β Gal, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gal+1/Beta+galactosidase+Antibody/pm41833832-60-30-32
    Average 95 stars, based on 84 article reviews
    anti β gal - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: Galectin-3-integrin α5β1 phase separation disrupted by advanced glycation end-products impairs diabetic wound healing in rodents
    Article Snippet: The following primary antibodies were used: Gal-3 (1:100, ab76245, Abcam, USA), Gal-1 (1:200, 11858-1-AP, Proteintech, China), Gal-7 (1:200, 16730-1-AP, Proteintech, China), CD31 (1:500, ab182981, Abcam, USA), AGEs (1:100, BS-1158R, Bioss, China), The following secondary antibodies were used: goat anti-mouse HRP-conjugated (1:500, CW0102, CWBIO, China), goat anti-rabbit HRP-conjugated (1:500, CW0103, CWBIO, China).

    Incubation:

    Article Title: Gal-1-mediated cytochrome p450 activation promotes fibroblast into myofibroblast differentiation in pulmonary fibrosis.
    Article Snippet: Pulmonary fibrosis (PF) results from excessive extracellular matrix (ECM) deposition and tissue remodeling after activation of fibroblasts into myofibroblasts.. Abnormally deposited fibrotic ECM, in turn, promotes fibroblast activation and accelerates loss of lung structure and function.. However, the molecular mediators and exact mechanisms by which fibrotic ECM promotes fibroblast activation are unclear.



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    Proteintech anti β gal
    ( A ) Quantified levels of IL-1α from naïve fibroblast (Naïve fibro), inflammatory fibroblast (Inflam fibro), and ectopic basal cell (Basal) conditioned media as determined by ELISA. ( B ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates and treated with a 5-fold titration of IL-1α added to control media, including the level determined in (A). ( C ) Graphic illustrating the strategy used to deplete conditioned media of endogenous IL-1α with antibodies, magnetic beads, and column-based separation. ( D ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates in control or conditioned media depleted using <t>IgG</t> (CTRL IgG or CM IgG) or IL-1α nAb (CTRL nAB, CM nAb) and supplemented with additional IL-1α nAb after depletion (+Spike). [(B) and (D)] Data shows mean fold change ± SD and are pooled from 3 independent experiments (n=3 mice). P values were calculated using either (B) a ratio paired t -test or (D) a one-way ANOVA with post-hoc Turkey multiple comparison test: * P < 0.05, ** P < 0.01, **** P < 0.0001, ns: not significant.
    Anti β Gal, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gal+1/Beta+galactosidase+Antibody/pm41833832-60-30-32
    Average 95 stars, based on 1 article reviews
    anti β gal - by Bioz Stars, 2026-09
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    Image Search Results


    Representative immunohistochemical staining of Gal-1 in placental tissues (DAB staining). ( A ) Normal pregnancy control group. ( B ) Early-onset FGR group. ( C ) Late-onset FGR group.

    Journal: International Journal of Women's Health

    Article Title: Expression Levels and Significance of Gal-1 in Peripheral Blood and Placental Tissues of Pregnant Women with Fetal Growth Restriction

    doi: 10.2147/IJWH.S586881

    Figure Lengend Snippet: Representative immunohistochemical staining of Gal-1 in placental tissues (DAB staining). ( A ) Normal pregnancy control group. ( B ) Early-onset FGR group. ( C ) Late-onset FGR group.

    Article Snippet: The primary antibody was rabbit anti-human monoclonal Gal-1 antibody (1:1000, Affinity Biosciences LTD), and the steps were strictly followed according to the kit instructions.

    Techniques: Immunohistochemical staining, Staining, Control

    Scatter plot showing the negative correlation between serum Gal-1 levels and relative placental Gal-1 mRNA expression levels.

    Journal: International Journal of Women's Health

    Article Title: Expression Levels and Significance of Gal-1 in Peripheral Blood and Placental Tissues of Pregnant Women with Fetal Growth Restriction

    doi: 10.2147/IJWH.S586881

    Figure Lengend Snippet: Scatter plot showing the negative correlation between serum Gal-1 levels and relative placental Gal-1 mRNA expression levels.

    Article Snippet: The primary antibody was rabbit anti-human monoclonal Gal-1 antibody (1:1000, Affinity Biosciences LTD), and the steps were strictly followed according to the kit instructions.

    Techniques: Expressing

    Receiver Operating Characteristic (ROC) curve analyzing the diagnostic performance of serum Gal-1 levels for distinguishing all FGR patients from healthy controls.

    Journal: International Journal of Women's Health

    Article Title: Expression Levels and Significance of Gal-1 in Peripheral Blood and Placental Tissues of Pregnant Women with Fetal Growth Restriction

    doi: 10.2147/IJWH.S586881

    Figure Lengend Snippet: Receiver Operating Characteristic (ROC) curve analyzing the diagnostic performance of serum Gal-1 levels for distinguishing all FGR patients from healthy controls.

    Article Snippet: The primary antibody was rabbit anti-human monoclonal Gal-1 antibody (1:1000, Affinity Biosciences LTD), and the steps were strictly followed according to the kit instructions.

    Techniques: Diagnostic Assay

    Analysis of galectin expression in the spleen. A – D : Galectin-1 (Gal-1) immunoreactivity. E–H : Galectin-3 (Gal-3) immunoreactivity. I–L : Galectin-9 (Gal-9) immunoreactivity. Immunolabeling is observed in both white pulp (wp) and red pulp (rp), with differences in distribution and intensity among groups: SHAM (control animals), MCP (animals treated with MCP), CIS (animals treated with cisplatin), and MCP + CIS (animals treated with MCP and cisplatin). Counterstain: Carazzi’s hematoxylin. Scale bars: 100 μm. M–O : Densitometric analysis of Gal-1, Gal-3, and Gal-9 immunoreactivity in splenic tissue. Data represent the mean ± SEM of arbitrary units (a.u.) of protein expression ( n = 5 animals/group). * p < 0.05; ** p < 0.01, *** p < 0.001; **** p < 0.0001 (M, O, Q-S: ANOVA followed by post-hoc Tukey test; N: Kruskal-Wallis followed by post-hoc Dunn’s test)

    Journal: Journal of Molecular Histology

    Article Title: Modified citrus pectin modulates splenic immune responses and galectin expression following cisplatin treatment in Wistar rats

    doi: 10.1007/s10735-026-10828-w

    Figure Lengend Snippet: Analysis of galectin expression in the spleen. A – D : Galectin-1 (Gal-1) immunoreactivity. E–H : Galectin-3 (Gal-3) immunoreactivity. I–L : Galectin-9 (Gal-9) immunoreactivity. Immunolabeling is observed in both white pulp (wp) and red pulp (rp), with differences in distribution and intensity among groups: SHAM (control animals), MCP (animals treated with MCP), CIS (animals treated with cisplatin), and MCP + CIS (animals treated with MCP and cisplatin). Counterstain: Carazzi’s hematoxylin. Scale bars: 100 μm. M–O : Densitometric analysis of Gal-1, Gal-3, and Gal-9 immunoreactivity in splenic tissue. Data represent the mean ± SEM of arbitrary units (a.u.) of protein expression ( n = 5 animals/group). * p < 0.05; ** p < 0.01, *** p < 0.001; **** p < 0.0001 (M, O, Q-S: ANOVA followed by post-hoc Tukey test; N: Kruskal-Wallis followed by post-hoc Dunn’s test)

    Article Snippet: A – D : Galectin-1 (Gal-1) immunoreactivity.

    Techniques: Expressing, Immunolabeling, Control

    Correlation analysis of galectin expression and splenic immune cell populations between CIS and MCP + CIS groups. A , B : Correlation analyses show no significant correlations between galectin expression (Gal-1, Gal-3, and Gal-9) and CD68⁺ macrophages or CD3⁺ T cells in the CIS group. C : Correlation analysis indicating positive associations between Gal-1, Gal-3, and Gal-9 expression and the CD68 + macrophage population in the MCP + CIS group. D : Correlation analysis showing a positive association between Gal-3 expression and CD3⁺ T cells in the MCP + CIS group. Correlation analyses were performed using Pearson or Spearman tests, depending on the data distribution ( n = 5 animals/group). * p < 0.05; *** p < 0.001; **** p < 0.0001

    Journal: Journal of Molecular Histology

    Article Title: Modified citrus pectin modulates splenic immune responses and galectin expression following cisplatin treatment in Wistar rats

    doi: 10.1007/s10735-026-10828-w

    Figure Lengend Snippet: Correlation analysis of galectin expression and splenic immune cell populations between CIS and MCP + CIS groups. A , B : Correlation analyses show no significant correlations between galectin expression (Gal-1, Gal-3, and Gal-9) and CD68⁺ macrophages or CD3⁺ T cells in the CIS group. C : Correlation analysis indicating positive associations between Gal-1, Gal-3, and Gal-9 expression and the CD68 + macrophage population in the MCP + CIS group. D : Correlation analysis showing a positive association between Gal-3 expression and CD3⁺ T cells in the MCP + CIS group. Correlation analyses were performed using Pearson or Spearman tests, depending on the data distribution ( n = 5 animals/group). * p < 0.05; *** p < 0.001; **** p < 0.0001

    Article Snippet: A – D : Galectin-1 (Gal-1) immunoreactivity.

    Techniques: Expressing

    ( A ) Quantified levels of IL-1α from naïve fibroblast (Naïve fibro), inflammatory fibroblast (Inflam fibro), and ectopic basal cell (Basal) conditioned media as determined by ELISA. ( B ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates and treated with a 5-fold titration of IL-1α added to control media, including the level determined in (A). ( C ) Graphic illustrating the strategy used to deplete conditioned media of endogenous IL-1α with antibodies, magnetic beads, and column-based separation. ( D ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates in control or conditioned media depleted using IgG (CTRL IgG or CM IgG) or IL-1α nAb (CTRL nAB, CM nAb) and supplemented with additional IL-1α nAb after depletion (+Spike). [(B) and (D)] Data shows mean fold change ± SD and are pooled from 3 independent experiments (n=3 mice). P values were calculated using either (B) a ratio paired t -test or (D) a one-way ANOVA with post-hoc Turkey multiple comparison test: * P < 0.05, ** P < 0.01, **** P < 0.0001, ns: not significant.

    Journal: bioRxiv

    Article Title: Dysplastic Epithelial Repair Propagates Chronic Pathology Through the Paracrine Transformation of Pulmonary Fibroblasts

    doi: 10.64898/2026.04.02.716135

    Figure Lengend Snippet: ( A ) Quantified levels of IL-1α from naïve fibroblast (Naïve fibro), inflammatory fibroblast (Inflam fibro), and ectopic basal cell (Basal) conditioned media as determined by ELISA. ( B ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates and treated with a 5-fold titration of IL-1α added to control media, including the level determined in (A). ( C ) Graphic illustrating the strategy used to deplete conditioned media of endogenous IL-1α with antibodies, magnetic beads, and column-based separation. ( D ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates in control or conditioned media depleted using IgG (CTRL IgG or CM IgG) or IL-1α nAb (CTRL nAB, CM nAb) and supplemented with additional IL-1α nAb after depletion (+Spike). [(B) and (D)] Data shows mean fold change ± SD and are pooled from 3 independent experiments (n=3 mice). P values were calculated using either (B) a ratio paired t -test or (D) a one-way ANOVA with post-hoc Turkey multiple comparison test: * P < 0.05, ** P < 0.01, **** P < 0.0001, ns: not significant.

    Article Snippet: The working concentration for the following reagents can be found in the text and figure legends: Human IL-1RA Recombinant Protein (IL1Ra) (PeproTech®, 200-01RA), Mouse IL-1 alpha Recombinant Protein, (PeproTech®, 211-11A), Mouse IL-1α Neutralizing Antibody (Invivogen, Anti-mIL-1α-mIgG1 clone 6H7, mIL-1α-mab9-02), Recombinant Mouse IgG1 Isotype Control Antibody (Invivogen, Anti-β-Gal-mIgG1 clone T9C6, bgal-mab9-02), Human TGF-β 1 Recombinant Protein (PeproTech®, 100-21), Human GDF15 Recombinant Protein (PeproTech®, 120-28C), Human VEGF Recombinant Protein (PeproTech®, 450-32), Human OPN Recombinant Protein (PeproTech®, 120-35), Murine CXCL1 Recombinant Protein (PeproTech®, 250-11), Human IGFBP3 Recombinant Protein (PeproTech®, 100-08), Human TNFRSF11B Recombinant Protein (PeproTech®, 450-14), Murine CXCL5 Recombinant Protein (PeproTech®, 250-17), and Muring CCL20 Recombinant Protein (PeproTech®, 250-27).

    Techniques: Enzyme-linked Immunosorbent Assay, Gene Expression, Quantitative RT-PCR, Cell Culture, Titration, Control, Magnetic Beads, Comparison