Journal: International Journal of Women's Health

Article Title: Expression Levels and Significance of Gal-1 in Peripheral Blood and Placental Tissues of Pregnant Women with Fetal Growth Restriction

doi: 10.2147/IJWH.S586881

Figure Lengend Snippet: Representative immunohistochemical staining of Gal-1 in placental tissues (DAB staining). ( A ) Normal pregnancy control group. ( B ) Early-onset FGR group. ( C ) Late-onset FGR group.

Article Snippet: The primary antibody was rabbit anti-human monoclonal Gal-1 antibody (1:1000, Affinity Biosciences LTD), and the steps were strictly followed according to the kit instructions.

Techniques: Immunohistochemical staining, Staining, Control

Journal: International Journal of Women's Health

Article Title: Expression Levels and Significance of Gal-1 in Peripheral Blood and Placental Tissues of Pregnant Women with Fetal Growth Restriction

doi: 10.2147/IJWH.S586881

Figure Lengend Snippet: Scatter plot showing the negative correlation between serum Gal-1 levels and relative placental Gal-1 mRNA expression levels.

Article Snippet: The primary antibody was rabbit anti-human monoclonal Gal-1 antibody (1:1000, Affinity Biosciences LTD), and the steps were strictly followed according to the kit instructions.

Techniques: Expressing

Journal: International Journal of Women's Health

Article Title: Expression Levels and Significance of Gal-1 in Peripheral Blood and Placental Tissues of Pregnant Women with Fetal Growth Restriction

doi: 10.2147/IJWH.S586881

Figure Lengend Snippet: Receiver Operating Characteristic (ROC) curve analyzing the diagnostic performance of serum Gal-1 levels for distinguishing all FGR patients from healthy controls.

Article Snippet: The primary antibody was rabbit anti-human monoclonal Gal-1 antibody (1:1000, Affinity Biosciences LTD), and the steps were strictly followed according to the kit instructions.

Techniques: Diagnostic Assay

Journal: Journal of Molecular Histology

Article Title: Modified citrus pectin modulates splenic immune responses and galectin expression following cisplatin treatment in Wistar rats

doi: 10.1007/s10735-026-10828-w

Figure Lengend Snippet: Analysis of galectin expression in the spleen. A – D : Galectin-1 (Gal-1) immunoreactivity. E–H : Galectin-3 (Gal-3) immunoreactivity. I–L : Galectin-9 (Gal-9) immunoreactivity. Immunolabeling is observed in both white pulp (wp) and red pulp (rp), with differences in distribution and intensity among groups: SHAM (control animals), MCP (animals treated with MCP), CIS (animals treated with cisplatin), and MCP + CIS (animals treated with MCP and cisplatin). Counterstain: Carazzi’s hematoxylin. Scale bars: 100 μm. M–O : Densitometric analysis of Gal-1, Gal-3, and Gal-9 immunoreactivity in splenic tissue. Data represent the mean ± SEM of arbitrary units (a.u.) of protein expression ( n = 5 animals/group). * p < 0.05; ** p < 0.01, *** p < 0.001; **** p < 0.0001 (M, O, Q-S: ANOVA followed by post-hoc Tukey test; N: Kruskal-Wallis followed by post-hoc Dunn’s test)

Article Snippet: A – D : Galectin-1 (Gal-1) immunoreactivity.

Techniques: Expressing, Immunolabeling, Control

Journal: Journal of Molecular Histology

Article Title: Modified citrus pectin modulates splenic immune responses and galectin expression following cisplatin treatment in Wistar rats

doi: 10.1007/s10735-026-10828-w

Figure Lengend Snippet: Correlation analysis of galectin expression and splenic immune cell populations between CIS and MCP + CIS groups. A , B : Correlation analyses show no significant correlations between galectin expression (Gal-1, Gal-3, and Gal-9) and CD68⁺ macrophages or CD3⁺ T cells in the CIS group. C : Correlation analysis indicating positive associations between Gal-1, Gal-3, and Gal-9 expression and the CD68 + macrophage population in the MCP + CIS group. D : Correlation analysis showing a positive association between Gal-3 expression and CD3⁺ T cells in the MCP + CIS group. Correlation analyses were performed using Pearson or Spearman tests, depending on the data distribution ( n = 5 animals/group). * p < 0.05; *** p < 0.001; **** p < 0.0001

Article Snippet: A – D : Galectin-1 (Gal-1) immunoreactivity.

Techniques: Expressing

Journal: bioRxiv

Article Title: Dysplastic Epithelial Repair Propagates Chronic Pathology Through the Paracrine Transformation of Pulmonary Fibroblasts

doi: 10.64898/2026.04.02.716135

Figure Lengend Snippet: ( A ) Quantified levels of IL-1α from naïve fibroblast (Naïve fibro), inflammatory fibroblast (Inflam fibro), and ectopic basal cell (Basal) conditioned media as determined by ELISA. ( B ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates and treated with a 5-fold titration of IL-1α added to control media, including the level determined in (A). ( C ) Graphic illustrating the strategy used to deplete conditioned media of endogenous IL-1α with antibodies, magnetic beads, and column-based separation. ( D ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates in control or conditioned media depleted using IgG (CTRL IgG or CM IgG) or IL-1α nAb (CTRL nAB, CM nAb) and supplemented with additional IL-1α nAb after depletion (+Spike). [(B) and (D)] Data shows mean fold change ± SD and are pooled from 3 independent experiments (n=3 mice). P values were calculated using either (B) a ratio paired t -test or (D) a one-way ANOVA with post-hoc Turkey multiple comparison test: * P < 0.05, ** P < 0.01, **** P < 0.0001, ns: not significant.

Article Snippet: The working concentration for the following reagents can be found in the text and figure legends: Human IL-1RA Recombinant Protein (IL1Ra) (PeproTech®, 200-01RA), Mouse IL-1 alpha Recombinant Protein, (PeproTech®, 211-11A), Mouse IL-1α Neutralizing Antibody (Invivogen, Anti-mIL-1α-mIgG1 clone 6H7, mIL-1α-mab9-02), Recombinant Mouse IgG1 Isotype Control Antibody (Invivogen, Anti-β-Gal-mIgG1 clone T9C6, bgal-mab9-02), Human TGF-β 1 Recombinant Protein (PeproTech®, 100-21), Human GDF15 Recombinant Protein (PeproTech®, 120-28C), Human VEGF Recombinant Protein (PeproTech®, 450-32), Human OPN Recombinant Protein (PeproTech®, 120-35), Murine CXCL1 Recombinant Protein (PeproTech®, 250-11), Human IGFBP3 Recombinant Protein (PeproTech®, 100-08), Human TNFRSF11B Recombinant Protein (PeproTech®, 450-14), Murine CXCL5 Recombinant Protein (PeproTech®, 250-17), and Muring CCL20 Recombinant Protein (PeproTech®, 250-27).

Techniques: Enzyme-linked Immunosorbent Assay, Gene Expression, Quantitative RT-PCR, Cell Culture, Titration, Control, Magnetic Beads, Comparison