67648 1 ig  (Proteintech)

Journal: iScience

Article Title: A role for nucleolin in functional improvement in stroke

doi: 10.1016/j.isci.2026.116134

Figure Lengend Snippet: Ischemic cortical stroke increases nucleolin expression in excitatory neurons (A) Representative coronal section stained with DAPI with stroke (red dashed line) and area of interest (yellow box). (B) Representative in situ hybridization images of DAPI (nuclear counterstain), Slc17a7 (excitatory cortical neurons), Gad1 (inhibitory neurons), and nucleolin at time points across acute to chronic stroke. The dashed box indicates the region shown at higher resolution and magnification in accompanying panels. Scale bars, 20 μm, n = 5 animals per time point. (C) Quantification of nucleolin mRNA expression in excitatory cortical neurons post-stroke. Stroke induces both early and chronic expression of nucleolin in Slc17a7 + cells. 1-way ANOVA with post-hoc Tukey correction. Data are represented as mean ± SEM with data points representing individual animals. ∗ p = 0.039, ∗∗∗ p = 0.003 (3D post stroke), p = 0.004 (7D post stroke). (D) Quantification of nucleolin mRNA expression in inhibitory cortical neurons post-stroke. No significant change in nucleolin expression was detected in Gad1 + cells. Data are represented as mean ± SEM with data points representing individual animals. (E) Representative images of nucleolin protein expression in sham stroke and 7 days post-stroke mice. Scale bars, 10 μm, n = 5 animals per group. (F) Quantification of nucleolin protein expression post-stroke. Stroke significantly increases nucleolin expression 7 days post-stroke ( p = 0.0005 via t test). Data are represented as mean ± SEM with data points representing individual animals.

Article Snippet: HCR 3.0 Gad1 , Molecular Instruments , N/A.

Techniques: Expressing, Staining, In Situ Hybridization

Journal: CytoJournal

Article Title: Gamma-aminobutyric acid affects the tumor microenvironment and angiogenesis, promoting breast cancer progression

doi: 10.25259/Cytojournal_143_2025

Figure Lengend Snippet: GAD1 is highly expressed in BC and promotes GABA synthesis. (a) Relative mRNA expression of GAD1 in MCF 10A cells and MAD-MB-231 cells was detected by qRT-PCR. (b and c) Relative protein expression of GAD1 in MCF 10A cells and MDA-MB-231 cells was detected by WB. (d) Levels of GABA in MCF 10A cells and MDA-MB-231 cells were detected by ELISA. (e) Expression of GAD1 in the normal population and cancer patients was analyzed on the UALCAN website. For in vitro assays, n = 6; for UALCAN analysis, normal n = 114 and primary tumor n = 1097; ✶ ✶ P <0.01, ✶ ✶ ✶ P <0.001. GAD1: Glutamic acid decarboxylase 1, BC: Breast cancer, GABA: Gamma-aminobutyric acid, mRNA: Messenger RNA, qRT-PCR: Quantitative real-time reverse transcription polymerase chain reaction, WB: Western blot, ELISA: Enzyme-linked immunosorbent assay, UALCAN: University of Alabama at Birmingham Cancer.

Article Snippet: GAD1 overexpression, GAD1 knockdown, and negative control plasmids were obtained from Sangon Biotech Co., Ltd. (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, In Vitro, Reverse Transcription, Polymerase Chain Reaction, Western Blot

Journal: CytoJournal

Article Title: Gamma-aminobutyric acid affects the tumor microenvironment and angiogenesis, promoting breast cancer progression

doi: 10.25259/Cytojournal_143_2025

Figure Lengend Snippet: Transfection efficiency was verified using qRT-PCR and WB. (a-c) Overexpression and knockdown efficiency of GAD1 were detected by qRT-PCR and WB. (d-f) Knockdown efficiency of GAD1 was detected by qRT-PCR and WB. (g and h) Levels of GABA were tested using an ELISA kit. n = 6; ✶ ✶ ✶ P <0.001. GAD1: Glutamic acid decarboxylase 1, qRT-PCR: Quantitative real-time reverse transcription polymerase chain reaction, WB: Western blot, ELISA: Enzyme-linked immunosorbent assay.

Article Snippet: GAD1 overexpression, GAD1 knockdown, and negative control plasmids were obtained from Sangon Biotech Co., Ltd. (Shanghai, China).

Techniques: Transfection, Quantitative RT-PCR, Over Expression, Knockdown, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Polymerase Chain Reaction, Western Blot

Journal: CytoJournal

Article Title: Gamma-aminobutyric acid affects the tumor microenvironment and angiogenesis, promoting breast cancer progression

doi: 10.25259/Cytojournal_143_2025

Figure Lengend Snippet: GAD1 is highly expressed in BC and promotes GABA synthesis. (a) Relative mRNA expression of GAD1 in MCF 10A cells and MAD-MB-231 cells was detected by qRT-PCR. (b and c) Relative protein expression of GAD1 in MCF 10A cells and MDA-MB-231 cells was detected by WB. (d) Levels of GABA in MCF 10A cells and MDA-MB-231 cells were detected by ELISA. (e) Expression of GAD1 in the normal population and cancer patients was analyzed on the UALCAN website. For in vitro assays, n = 6; for UALCAN analysis, normal n = 114 and primary tumor n = 1097; ✶ ✶ P <0.01, ✶ ✶ ✶ P <0.001. GAD1: Glutamic acid decarboxylase 1, BC: Breast cancer, GABA: Gamma-aminobutyric acid, mRNA: Messenger RNA, qRT-PCR: Quantitative real-time reverse transcription polymerase chain reaction, WB: Western blot, ELISA: Enzyme-linked immunosorbent assay, UALCAN: University of Alabama at Birmingham Cancer.

Article Snippet: GAD1 overexpression, GAD1 knockdown, and negative control plasmids were obtained from Sangon Biotech Co., Ltd. (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, In Vitro, Reverse Transcription, Polymerase Chain Reaction, Western Blot

Journal: CytoJournal

Article Title: Gamma-aminobutyric acid affects the tumor microenvironment and angiogenesis, promoting breast cancer progression

doi: 10.25259/Cytojournal_143_2025

Figure Lengend Snippet: Transfection efficiency was verified using qRT-PCR and WB. (a-c) Overexpression and knockdown efficiency of GAD1 were detected by qRT-PCR and WB. (d-f) Knockdown efficiency of GAD1 was detected by qRT-PCR and WB. (g and h) Levels of GABA were tested using an ELISA kit. n = 6; ✶ ✶ ✶ P <0.001. GAD1: Glutamic acid decarboxylase 1, qRT-PCR: Quantitative real-time reverse transcription polymerase chain reaction, WB: Western blot, ELISA: Enzyme-linked immunosorbent assay.

Article Snippet: GAD1 overexpression, GAD1 knockdown, and negative control plasmids were obtained from Sangon Biotech Co., Ltd. (Shanghai, China).

Techniques: Transfection, Quantitative RT-PCR, Over Expression, Knockdown, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Polymerase Chain Reaction, Western Blot