fty720  (MedChemExpress)

Journal: bioRxiv

Article Title: Indomethacin exerts both cyclooxygenase inhibition-dependent and independent mechanisms to enhance chemo-immunotherapy in mice

doi: 10.64898/2026.01.07.698231

Figure Lengend Snippet: (A) Indo fails to enhance CTX efficacy in immunodeficient NSG mice. The schema depicts the timeline of experimental procedures. NSG mice bearing established CT26 tumors (60–90 mm²) were randomized into four groups and treated as indicated. Tumor growth curves are shown. (B) Kaplan–Meier survival analysis of the same cohort. Data were pooled from two independent experiments. (C) The beneficial effect of Indo requires endogenous CD8⁺ T cells. The schema depicts the timeline of experimental procedures. BALB/c mice bearing established CT26 tumors (60–90 mm²) were treated with CTX+Indo. A subset of mice additionally received i.p. anti-CD8 Ab before and during treatment to deplete endogenous CD8⁺ T cells. Mice treated with CTX alone were included as controls. Tumor growth curves are shown. Mouse survival is summarized in the Kaplan-Meier plot (D). (E) Blockade of T cell trafficking by FTY720 diminishes the efficacy of CTX+Indo. BALB/c mice with established CT26 tumors (60–90 mm²) were treated with CTX+Indo. These mice also received either FTY720 or solvent by i.p. injection three times weekly for 4 weeks, starting one day prior to CTX administration. Tumor growth curves are shown. (F) Kaplan–Meier survival analysis. Data were pooled from two independent experiments. Data were pooled from two independent experiments. Statistics: (B, D, F) Log-rank (Mantel-Cox) test. **, p < 0.01; ***, p < 0.001; ns, not significant.

Article Snippet: Fingolimod hydrochloride (FTY720) was purchased from MedChemExpress.

Techniques: Solvent, Injection

Journal: bioRxiv

Article Title: Indomethacin exerts both cyclooxygenase inhibition-dependent and independent mechanisms to enhance chemo-immunotherapy in mice

doi: 10.64898/2026.01.07.698231

Figure Lengend Snippet: (A) FTY720 administration reduces T cells in circulation but does not alter T cell frequency in secondary lymphoid organs. CT26-bearing BALB/c mice were treated as described in . A cohort of mice were euthanized after receiving 4 doses of FTY720 or solvent. The frequencies of CD4+ and CD8+ T cells in blood, draining lymph node and spleen were determined by flow cytometry. Representative dot plots are shown. Numbers in plots denote percent of CD4+ and CD8+ T cells. (B) FTY720 administration diminishes the efficacy of CTX+Indo in MC38 tumor model. Following the same experimental procedures depicted in , C57BL/6 mice with established MC38 tumors (60-90mm 2 ) were treated as indicated. Tumor growth curves are shown with mice numbers in each group indicated. Mouse survival is summarized in the Kaplan-Meier plot (C). Statistics: Log-rank (Mantel-Cox) test. *, p < 0.05; ns, not significant.

Article Snippet: Fingolimod hydrochloride (FTY720) was purchased from MedChemExpress.

Techniques: Solvent, Flow Cytometry