fth1  (Cusabio)

Journal: Regenerative Therapy

Article Title: The umbilical cord blood exosome MFG-E8 alleviates hypoxic-ischemic encephalopathy brain injury in neonatal rats by restoring autophagy flux and inhibiting ferroptosis through GSK3β/β-catenin signaling

doi: 10.1016/j.reth.2025.06.016

Figure Lengend Snippet: HUBC treatment inhibited hyperautophagy and ferroptosis in neonatal rats with HIE. (A) Western blot detection of Beclin1, ATG7, LC3 II/I, and p62 expression in brain tissue. (B) Western blot analysis of NCOA4, ACSL4, FTH1, SLC7A11, and GPX4 expression in brain tissue. (C) Biochemical detection of MDA and Fe 2+ concentration. (D) Flow cytometry detection of ROS level in brain tissue. (E) IF detection of the number of NeuN-positive cells. n = 6. ∗p < 0.05 vs. Sham group; #p < 0.05 vs. HIE group.

Article Snippet: FTH1 , #4393 , Rabbit , 1:1000 , 21 KDa , CST , USA.

Techniques: Western Blot, Expressing, Concentration Assay, Flow Cytometry

Journal: Regenerative Therapy

Article Title: The umbilical cord blood exosome MFG-E8 alleviates hypoxic-ischemic encephalopathy brain injury in neonatal rats by restoring autophagy flux and inhibiting ferroptosis through GSK3β/β-catenin signaling

doi: 10.1016/j.reth.2025.06.016

Figure Lengend Snippet: HUBC-Exo treatment inhibited hyperautophagy and ferroptosis in HIE neonatal rats and increased MFG-E8 expression. (A) The morphology of exosomes was observed by TEM. (B) NTA particle size analysis. (C) Western blot assessment of exosome surface markers CD9, CD63, and TSG101. (D) Exosome uptake was detected by PKH67-labeling. (E) Western blot assessment of Beclin1, ATG7, LC3 II/I, and p62 expression. (F) Western blot analysis of NCOA4, ACSL4, FTH1, SLC7A11 and GPX4 levels. (G) The concentrations of MDA and Fe 2+ were detected by biochemical tests. (H) IF detection of ROS level in brain tissue. (I) IF detection of the number of NeuN-positive cells in brain tissue. (J) MFG-E8 expression in brain tissue was examined by IHC. n = 6. ∗p < 0.05 vs. Sham group; #p < 0.05 vs. HIE group.

Article Snippet: FTH1 , #4393 , Rabbit , 1:1000 , 21 KDa , CST , USA.

Techniques: Expressing, Particle Size Analysis, Western Blot, Labeling

Journal: Regenerative Therapy

Article Title: The umbilical cord blood exosome MFG-E8 alleviates hypoxic-ischemic encephalopathy brain injury in neonatal rats by restoring autophagy flux and inhibiting ferroptosis through GSK3β/β-catenin signaling

doi: 10.1016/j.reth.2025.06.016

Figure Lengend Snippet: HUBC-Exo-derived MFG-E8 inhibited hyperautophagy and ferroptosis in SH-SY5Y cells that had been subjected to OGD/R. (A) Western blot detection of MFG-E8 level. (B) Western blot analysis of MFG-E8 level. (C) CCK-8 detection of cell viability. (D) Biochemical detection of LDH level in supernatant. (E) Western blot detection of Beclin1, ATG7, LC3 II/I, and p62 expression. (F) Western blot analysis of NCOA4, ACSL4, FTH1, SLC7A11 and GPX4 level. (G) Biochemical detection of MDA, 4-HNE, and Fe 2+ concentration. (H) Lipid ROS level detection of flow cytometry. n = 3. ∗p < 0.05 vs. Control group; #p < 0.05 vs. OGD/R group; &p < 0.05 vs. OGD/R + HUBC-Exo si-NC group.

Article Snippet: FTH1 , #4393 , Rabbit , 1:1000 , 21 KDa , CST , USA.

Techniques: Derivative Assay, Western Blot, CCK-8 Assay, Expressing, Concentration Assay, Flow Cytometry, Control

Journal: Regenerative Therapy

Article Title: The umbilical cord blood exosome MFG-E8 alleviates hypoxic-ischemic encephalopathy brain injury in neonatal rats by restoring autophagy flux and inhibiting ferroptosis through GSK3β/β-catenin signaling

doi: 10.1016/j.reth.2025.06.016

Figure Lengend Snippet: HUBC-Exo-derived MFG-E8 inhibited hyperautophagy and ferroptosis in SH-SY5Y cells that had been subjected to OGD/R through GSK3β/β-catenin signaling. (A) Western blot assessment of p-GSK3β, GSK3β, Active-β-catenin, and β-catenin expression. (B) MFG-E8, p-GSK3β, GSK3β, Active-β-catenin, and β-catenin expression were detected by Western blot. (C) CCK-8 detection of cell viability. (D) Biochemical detection of LDH level in supernatant. (E) Western blot assessment of Beclin1, ATG7, LC3 II/I, and p62 expression. (F) Western blot detection of NCOA4, ACSL4, FTH1, SLC7A11 and GPX4 expression. (G) Biochemical detection of MDA, 4-HNE, and Fe 2+ concentration. (H) Lipid ROS levels assessment of flow cytometry. n = 3. ∗p < 0.05 vs. Control group; #p < 0.05 vs. OGD/R group; &p < 0.05 vs. OGD/R + HUBC-Exo si-NC group; @p < 0.05 vs. OGD/R + HUBC-Exo si-MFG-E8 group.

Article Snippet: FTH1 , #4393 , Rabbit , 1:1000 , 21 KDa , CST , USA.

Techniques: Derivative Assay, Western Blot, Expressing, CCK-8 Assay, Concentration Assay, Flow Cytometry, Control

Journal: Regenerative Therapy

Article Title: The umbilical cord blood exosome MFG-E8 alleviates hypoxic-ischemic encephalopathy brain injury in neonatal rats by restoring autophagy flux and inhibiting ferroptosis through GSK3β/β-catenin signaling

doi: 10.1016/j.reth.2025.06.016

Figure Lengend Snippet: HUBC-Exo-derived MFG-E8 reduced autophagy and ferroptosis in neonatal rats with HIE. (A) Western blot assessment of Beclin1, ATG7, LC3 II/I, and p62 expression. (B) Western blot analysis of NCOA4, ACSL4, FTH1, SLC7A11 and GPX4 expression. (C) The concentrations of MDA and Fe 2+ were determined by biochemical tests. (D) Flow cytometry detection of ROS level in brain tissue. (E) IF detection of the number of surviving neurons in brain tissue. n = 6. ∗p < 0.05 vs. Sham group; #p < 0.05 vs. HIE group; &p < 0.05 vs. HIE + HUBC-Exo si-NC group.

Article Snippet: FTH1 , #4393 , Rabbit , 1:1000 , 21 KDa , CST , USA.

Techniques: Derivative Assay, Western Blot, Expressing, Flow Cytometry