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ft709  (MedChemExpress)


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    Structured Review

    MedChemExpress ft709
    A Number of unique peptides and their coverage obtained in USP9x and PYCR3 from LC-MS/MS analysis on co-immunoprecipitation assay. B Co-immunoprecipitates from ( A ) resolved on western blot, normal rabbit IgG was used as control. C Ubiquitination assay showing the levels of ubiquitinated PYCR3 in USP9x overexpression vs. control upon treatment with MG132 for 4 h. Bar graph shows quantification of ubiquitinated PYCR3 normalized to total PYCR3 levels and the control. D siRNA-mediated knockdown of USP9x, as well as ( E ) USP9x inhibition with the specific inhibitor <t>FT709</t> for 48 h reduced PYCR3 protein levels. F USP9x overexpression elevated PYCR3 protein level. G PYCR3 transcription upon USP9x siRNA-mediated knockdown remained unchanged. All quantifications pooled from 3 independent biological replicates. Error bars, ± SD. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.
    Ft709, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ft709/product/MedChemExpress
    Average 93 stars, based on 2 article reviews
    ft709 - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "Deubiquitinase USP9x regulates the proline biosynthesis pathway in non-small cell lung cancer"

    Article Title: Deubiquitinase USP9x regulates the proline biosynthesis pathway in non-small cell lung cancer

    Journal: Cell Death Discovery

    doi: 10.1038/s41420-024-02111-2

    A Number of unique peptides and their coverage obtained in USP9x and PYCR3 from LC-MS/MS analysis on co-immunoprecipitation assay. B Co-immunoprecipitates from ( A ) resolved on western blot, normal rabbit IgG was used as control. C Ubiquitination assay showing the levels of ubiquitinated PYCR3 in USP9x overexpression vs. control upon treatment with MG132 for 4 h. Bar graph shows quantification of ubiquitinated PYCR3 normalized to total PYCR3 levels and the control. D siRNA-mediated knockdown of USP9x, as well as ( E ) USP9x inhibition with the specific inhibitor FT709 for 48 h reduced PYCR3 protein levels. F USP9x overexpression elevated PYCR3 protein level. G PYCR3 transcription upon USP9x siRNA-mediated knockdown remained unchanged. All quantifications pooled from 3 independent biological replicates. Error bars, ± SD. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.
    Figure Legend Snippet: A Number of unique peptides and their coverage obtained in USP9x and PYCR3 from LC-MS/MS analysis on co-immunoprecipitation assay. B Co-immunoprecipitates from ( A ) resolved on western blot, normal rabbit IgG was used as control. C Ubiquitination assay showing the levels of ubiquitinated PYCR3 in USP9x overexpression vs. control upon treatment with MG132 for 4 h. Bar graph shows quantification of ubiquitinated PYCR3 normalized to total PYCR3 levels and the control. D siRNA-mediated knockdown of USP9x, as well as ( E ) USP9x inhibition with the specific inhibitor FT709 for 48 h reduced PYCR3 protein levels. F USP9x overexpression elevated PYCR3 protein level. G PYCR3 transcription upon USP9x siRNA-mediated knockdown remained unchanged. All quantifications pooled from 3 independent biological replicates. Error bars, ± SD. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.

    Techniques Used: Liquid Chromatography with Mass Spectroscopy, Co-Immunoprecipitation Assay, Western Blot, Control, Ubiquitin Assay, Over Expression, Knockdown, Inhibition



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    A Number of unique peptides and their coverage obtained in USP9x and PYCR3 from LC-MS/MS analysis on co-immunoprecipitation assay. B Co-immunoprecipitates from ( A ) resolved on western blot, normal rabbit IgG was used as control. C Ubiquitination assay showing the levels of ubiquitinated PYCR3 in USP9x overexpression vs. control upon treatment with MG132 for 4 h. Bar graph shows quantification of ubiquitinated PYCR3 normalized to total PYCR3 levels and the control. D siRNA-mediated knockdown of USP9x, as well as ( E ) USP9x inhibition with the specific inhibitor <t>FT709</t> for 48 h reduced PYCR3 protein levels. F USP9x overexpression elevated PYCR3 protein level. G PYCR3 transcription upon USP9x siRNA-mediated knockdown remained unchanged. All quantifications pooled from 3 independent biological replicates. Error bars, ± SD. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.
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    A Number of unique peptides and their coverage obtained in USP9x and PYCR3 from LC-MS/MS analysis on co-immunoprecipitation assay. B Co-immunoprecipitates from ( A ) resolved on western blot, normal rabbit IgG was used as control. C Ubiquitination assay showing the levels of ubiquitinated PYCR3 in USP9x overexpression vs. control upon treatment with MG132 for 4 h. Bar graph shows quantification of ubiquitinated PYCR3 normalized to total PYCR3 levels and the control. D siRNA-mediated knockdown of USP9x, as well as ( E ) USP9x inhibition with the specific inhibitor <t>FT709</t> for 48 h reduced PYCR3 protein levels. F USP9x overexpression elevated PYCR3 protein level. G PYCR3 transcription upon USP9x siRNA-mediated knockdown remained unchanged. All quantifications pooled from 3 independent biological replicates. Error bars, ± SD. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.
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    A Number of unique peptides and their coverage obtained in USP9x and PYCR3 from LC-MS/MS analysis on co-immunoprecipitation assay. B Co-immunoprecipitates from ( A ) resolved on western blot, normal rabbit IgG was used as control. C Ubiquitination assay showing the levels of ubiquitinated PYCR3 in USP9x overexpression vs. control upon treatment with MG132 for 4 h. Bar graph shows quantification of ubiquitinated PYCR3 normalized to total PYCR3 levels and the control. D siRNA-mediated knockdown of USP9x, as well as ( E ) USP9x inhibition with the specific inhibitor <t>FT709</t> for 48 h reduced PYCR3 protein levels. F USP9x overexpression elevated PYCR3 protein level. G PYCR3 transcription upon USP9x siRNA-mediated knockdown remained unchanged. All quantifications pooled from 3 independent biological replicates. Error bars, ± SD. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.
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    Image Search Results


    A Number of unique peptides and their coverage obtained in USP9x and PYCR3 from LC-MS/MS analysis on co-immunoprecipitation assay. B Co-immunoprecipitates from ( A ) resolved on western blot, normal rabbit IgG was used as control. C Ubiquitination assay showing the levels of ubiquitinated PYCR3 in USP9x overexpression vs. control upon treatment with MG132 for 4 h. Bar graph shows quantification of ubiquitinated PYCR3 normalized to total PYCR3 levels and the control. D siRNA-mediated knockdown of USP9x, as well as ( E ) USP9x inhibition with the specific inhibitor FT709 for 48 h reduced PYCR3 protein levels. F USP9x overexpression elevated PYCR3 protein level. G PYCR3 transcription upon USP9x siRNA-mediated knockdown remained unchanged. All quantifications pooled from 3 independent biological replicates. Error bars, ± SD. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.

    Journal: Cell Death Discovery

    Article Title: Deubiquitinase USP9x regulates the proline biosynthesis pathway in non-small cell lung cancer

    doi: 10.1038/s41420-024-02111-2

    Figure Lengend Snippet: A Number of unique peptides and their coverage obtained in USP9x and PYCR3 from LC-MS/MS analysis on co-immunoprecipitation assay. B Co-immunoprecipitates from ( A ) resolved on western blot, normal rabbit IgG was used as control. C Ubiquitination assay showing the levels of ubiquitinated PYCR3 in USP9x overexpression vs. control upon treatment with MG132 for 4 h. Bar graph shows quantification of ubiquitinated PYCR3 normalized to total PYCR3 levels and the control. D siRNA-mediated knockdown of USP9x, as well as ( E ) USP9x inhibition with the specific inhibitor FT709 for 48 h reduced PYCR3 protein levels. F USP9x overexpression elevated PYCR3 protein level. G PYCR3 transcription upon USP9x siRNA-mediated knockdown remained unchanged. All quantifications pooled from 3 independent biological replicates. Error bars, ± SD. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.

    Article Snippet: FT709, a specific inhibitor of USP9x [ ] was purchased from MedChemExpress (HY-145967, Monmouth Junction, NJ, USA) and prepared according to the manufacturer’s instructions.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Co-Immunoprecipitation Assay, Western Blot, Control, Ubiquitin Assay, Over Expression, Knockdown, Inhibition