bs 23042r  (Bioss)

Journal: Science Advances

Article Title: Hcn1-dependent engram neurons in the PVN encode gastric inflammatory sensitization

doi: 10.1126/sciadv.aeb6961

Figure Lengend Snippet: ( A ) Schematic of the HCl/EtOH-induced gastritis model. h, hours. ( B ) Gross morphology of gastric tissues. ( C and D ) Histopathological analysis (C: H&E staining; D: mucosal injury score). ( E ) Elevated IL-1β and TNF-α levels in gastric tissues of gastritis mice. ( F ) TdTomato (TdT) expression in activated neurons post–4-OHT treatment. ( G ) Experimental workflow for PRV retrograde tracing in Fos-CreER T2 × Rosa-TdT mice. ( H ) Whole-brain mapping of PRV-EGFP + neurons in the saline group and the gastritis group in Fos-CreER T2 × Rosa-TdT mice. ( I ) Quantification of c-Fos/PRV-EGFP colocalization across brain regions in the saline group and the gastritis group. ( J ) Representative c-Fos immunofluorescence images of the saline group and the gastritis group. ( K ) c-Fos + neuron counts in gastritis versus control [ n = 6 mice; F (5,55) = 123.4, two-way ANOVA].

Article Snippet: Fos-CreER T2 mice (the Jackson Laboratory, strain no. 030323) were crossed with Rosa-TdTomato reporter mice (the Jackson Laboratory, strain no. 007914) to generate Fos-CreER T2 × Rosa-TdT mice for neuronal labeling.

Techniques: Staining, Expressing, Retrograde Tracing, Saline, Immunofluorescence, Control

Journal: Science Advances

Article Title: Hcn1-dependent engram neurons in the PVN encode gastric inflammatory sensitization

doi: 10.1126/sciadv.aeb6961

Figure Lengend Snippet: ( A ) Schematic of the water-immersion RS-induced gastritis model. ( B ) Gross morphological appearance of gastric tissues in mice. ( C and D ) Gastric mucosal injury scores and representative H&E-stained sections ( n = 6 mice; two-tailed unpaired t test). ( E ) Stress significantly elevated levels of inflammatory cytokines IL-1β and TNF-α in gastric tissues compared to controls ( n = 6; two-tailed t test). ( F ) Representative Fos immunofluorescence images in PVN from control and stressed mice. ( G ) Increased Fos expression in the PVN of stressed mice versus controls ( n = 6; two-tailed t test). ( H ) Elevated serum corticosterone levels in stressed mice ( n = 7; two-tailed t test). ( I and J ) Enhanced gastric nerve firing frequency following stress exposure ( n = 5; two-tailed t test). ( K ) Experimental timeline for PRV retrograde tracing in Fos-CreER T2 × Rosa-TdT mice subjected to RS. ( L ) Representative images showing PRV-EGFP + neurons colocalized with Fos + cells across brain regions. ( M ) Quantification of Fos/PRV colocalization rates in different brain areas.

Article Snippet: Fos-CreER T2 mice (the Jackson Laboratory, strain no. 030323) were crossed with Rosa-TdTomato reporter mice (the Jackson Laboratory, strain no. 007914) to generate Fos-CreER T2 × Rosa-TdT mice for neuronal labeling.

Techniques: Staining, Two Tailed Test, Immunofluorescence, Control, Expressing, Retrograde Tracing

Journal: bioRxiv

Article Title: Neuromodulation of Foxp2 + hypothalamic neurons induces therapeutic hypothermia

doi: 10.64898/2026.05.04.722579

Figure Lengend Snippet: a , Average relative cerebral blood volume changes (ΔrCBV) of different region across the brain within 5 to 10 minutes after administration P57 compared to vehicle. b, 3D rendering of ΔrCBV of different hypothalamic nuclei within 5 to 10 minutes after administration P57 compared to vehicle. c, Different views of the 3D rendering results of various P57-activated brain regions in the hypothalamus within 5 to 10 minutes after administration. d, Schematic of labeling P57 activated neurons using Fos-CreERT2;tdTomato mice (n=6 mice for each group). e, f, Number of tdTomato + neurons across different hypothalamic nuclei (e) and representative slice (top) and quantification (bottom) of MPA and PVH (f) in P57 group and Vehicle group in Fos-CreERT2;tdTomato mice. Student’s t test used was two-sided. Heat-map values are means; floating bars show min to max and mean (line). Bar chart and error bars are presented as mean values ± s.e.m. Median eminence (ME), Paraventricular hypothalamic nucleus (PVH), Hypothalamic medial zone (MEZ),

Article Snippet: For whole brain imaging and virus injection, we used adult (6-10-week-old) male and female Fos-CreERT2;tdTomato mice (Jackson Lab), Fos-CreERT2 mice (Jackson Lab), and Foxp2-Cre mice (Jackson Lab).

Techniques: Labeling

Journal: bioRxiv

Article Title: Neuromodulation of Foxp2 + hypothalamic neurons induces therapeutic hypothermia

doi: 10.64898/2026.05.04.722579

Figure Lengend Snippet: Representative slice of posterior hypothalamic nucleus (PH), dorsomedial nucleus (DMH) and mammillary body (MM) in P57 group and Vehicle group in Fos-CreERT2;tdTomato mice.

Article Snippet: For whole brain imaging and virus injection, we used adult (6-10-week-old) male and female Fos-CreERT2;tdTomato mice (Jackson Lab), Fos-CreERT2 mice (Jackson Lab), and Foxp2-Cre mice (Jackson Lab).

Techniques: