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z vad fmk  (MedChemExpress)


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    MedChemExpress z vad fmk
    Z Vad Fmk, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1072 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/z vad fmk/product/MedChemExpress
    Average 97 stars, based on 1072 article reviews
    z vad fmk - by Bioz Stars, 2026-05
    97/100 stars

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    Monitoring of apoptosis reporter in live cells. (A) HEK293 EGFP #4-8 cells were cultured with or without 1 μM STA, and images were obtained every 30 min for 48 h; scale bar 50 μm. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). (B) Protein levels of GFP, caspase-3, cleaved caspase-3, cytochrome C , PARP, and GAPDH by Western blotting in HEK293 EGFP #4-8 cells incubated with STA. Graph, protein levels (N = 3). (C) HEK293 EGFP #4-8 cells were cultured with 1 μM STA co-treated with or without 20 μM Z-DEVD-FMK and 10 μM <t>Z-YVAD-FMK,</t> and images were obtained every 30 min for 48 h. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). (D) HEK293 EGFP #4-8 cells were cultured with 100, 200, and 500 μM H 2 O 2 , and images were obtained every 30 min for 24 h. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). Error bars are ± SD. *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001.
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    Monitoring of apoptosis reporter in live cells. (A) HEK293 EGFP #4-8 cells were cultured with or without 1 μM STA, and images were obtained every 30 min for 48 h; scale bar 50 μm. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). (B) Protein levels of GFP, caspase-3, cleaved caspase-3, cytochrome C , PARP, and GAPDH by Western blotting in HEK293 EGFP #4-8 cells incubated with STA. Graph, protein levels (N = 3). (C) HEK293 EGFP #4-8 cells were cultured with 1 μM STA co-treated with or without 20 μM Z-DEVD-FMK and 10 μM <t>Z-YVAD-FMK,</t> and images were obtained every 30 min for 48 h. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). (D) HEK293 EGFP #4-8 cells were cultured with 100, 200, and 500 μM H 2 O 2 , and images were obtained every 30 min for 24 h. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). Error bars are ± SD. *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001.
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    z ietd fmk  (Selleck Chemicals)
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    Monitoring of apoptosis reporter in live cells. (A) HEK293 EGFP #4-8 cells were cultured with or without 1 μM STA, and images were obtained every 30 min for 48 h; scale bar 50 μm. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). (B) Protein levels of GFP, caspase-3, cleaved caspase-3, cytochrome C , PARP, and GAPDH by Western blotting in HEK293 EGFP #4-8 cells incubated with STA. Graph, protein levels (N = 3). (C) HEK293 EGFP #4-8 cells were cultured with 1 μM STA co-treated with or without <t>20</t> <t>μM</t> <t>Z-DEVD-FMK</t> and 10 μM Z-YVAD-FMK, and images were obtained every 30 min for 48 h. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). (D) HEK293 EGFP #4-8 cells were cultured with 100, 200, and 500 μM H 2 O 2 , and images were obtained every 30 min for 24 h. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). Error bars are ± SD. *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001.
    Z Ietd Fmk, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Monitoring of apoptosis reporter in live cells. (A) HEK293 EGFP #4-8 cells were cultured with or without 1 μM STA, and images were obtained every 30 min for 48 h; scale bar 50 μm. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). (B) Protein levels of GFP, caspase-3, cleaved caspase-3, cytochrome C , PARP, and GAPDH by Western blotting in HEK293 EGFP #4-8 cells incubated with STA. Graph, protein levels (N = 3). (C) HEK293 EGFP #4-8 cells were cultured with 1 μM STA co-treated with or without 20 μM Z-DEVD-FMK and 10 μM Z-YVAD-FMK, and images were obtained every 30 min for 48 h. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). (D) HEK293 EGFP #4-8 cells were cultured with 100, 200, and 500 μM H 2 O 2 , and images were obtained every 30 min for 24 h. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). Error bars are ± SD. *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001.

    Journal: Journal of Advanced Research

    Article Title: Designing an apoptosis reporter by mutagenesis-based insertion of caspase-3 cleavage motif into green fluorescence protein

    doi: 10.1016/j.jare.2025.06.070

    Figure Lengend Snippet: Monitoring of apoptosis reporter in live cells. (A) HEK293 EGFP #4-8 cells were cultured with or without 1 μM STA, and images were obtained every 30 min for 48 h; scale bar 50 μm. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). (B) Protein levels of GFP, caspase-3, cleaved caspase-3, cytochrome C , PARP, and GAPDH by Western blotting in HEK293 EGFP #4-8 cells incubated with STA. Graph, protein levels (N = 3). (C) HEK293 EGFP #4-8 cells were cultured with 1 μM STA co-treated with or without 20 μM Z-DEVD-FMK and 10 μM Z-YVAD-FMK, and images were obtained every 30 min for 48 h. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). (D) HEK293 EGFP #4-8 cells were cultured with 100, 200, and 500 μM H 2 O 2 , and images were obtained every 30 min for 24 h. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). Error bars are ± SD. *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001.

    Article Snippet: Z-DEVD-FMK (caspase-3 inhibitor, #sc-311558) and Z-YVAD-FMK (caspase-1 inhibitor, #sc-3071) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Techniques: Cell Culture, Fluorescence, Software, Western Blot, Incubation

    Monitoring of apoptosis reporter in live cells. (A) HEK293 EGFP #4-8 cells were cultured with or without 1 μM STA, and images were obtained every 30 min for 48 h; scale bar 50 μm. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). (B) Protein levels of GFP, caspase-3, cleaved caspase-3, cytochrome C , PARP, and GAPDH by Western blotting in HEK293 EGFP #4-8 cells incubated with STA. Graph, protein levels (N = 3). (C) HEK293 EGFP #4-8 cells were cultured with 1 μM STA co-treated with or without 20 μM Z-DEVD-FMK and 10 μM Z-YVAD-FMK, and images were obtained every 30 min for 48 h. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). (D) HEK293 EGFP #4-8 cells were cultured with 100, 200, and 500 μM H 2 O 2 , and images were obtained every 30 min for 24 h. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). Error bars are ± SD. *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001.

    Journal: Journal of Advanced Research

    Article Title: Designing an apoptosis reporter by mutagenesis-based insertion of caspase-3 cleavage motif into green fluorescence protein

    doi: 10.1016/j.jare.2025.06.070

    Figure Lengend Snippet: Monitoring of apoptosis reporter in live cells. (A) HEK293 EGFP #4-8 cells were cultured with or without 1 μM STA, and images were obtained every 30 min for 48 h; scale bar 50 μm. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). (B) Protein levels of GFP, caspase-3, cleaved caspase-3, cytochrome C , PARP, and GAPDH by Western blotting in HEK293 EGFP #4-8 cells incubated with STA. Graph, protein levels (N = 3). (C) HEK293 EGFP #4-8 cells were cultured with 1 μM STA co-treated with or without 20 μM Z-DEVD-FMK and 10 μM Z-YVAD-FMK, and images were obtained every 30 min for 48 h. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). (D) HEK293 EGFP #4-8 cells were cultured with 100, 200, and 500 μM H 2 O 2 , and images were obtained every 30 min for 24 h. Relative EGFP fluorescence intensity was measured using ImageJ software (N = 3). Error bars are ± SD. *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001.

    Article Snippet: Z-DEVD-FMK (caspase-3 inhibitor, #sc-311558) and Z-YVAD-FMK (caspase-1 inhibitor, #sc-3071) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Techniques: Cell Culture, Fluorescence, Software, Western Blot, Incubation