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fluoroshield mounting medium with dapi  (Beyotime)


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    Structured Review

    Beyotime fluoroshield mounting medium with dapi
    Fluoroshield Mounting Medium With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 31805 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluoroshield+dapi/DAPI/pmc13124985-211-29-34
    Average 99 stars, based on 31805 article reviews
    fluoroshield mounting medium with dapi - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Confocal Microscopy:

    Article Title: Engineered bacteriophage nanoassemblies in vivo stabilize DC-T cell immune synapse for high-performance influenza vaccination.
    Article Snippet: T cell activation by dendritic cells (DCs) requires the formation of a stable immune synapse (IS).. The objective of this study was to develop a vaccination approach by targetedly regulating DC–T cell synaptic interactions in vivo.. We constructed bacteriophage nanoassemblies to deliver antigen-encoding sequences into DC cytoplasm and enhance DC–T cell IS stability in vivo, thereby boosting vaccine potency.

    Incubation:

    Article Title: Shengjiang Xiexin Decoction ameliorates antibiotic-associated diarrhea by altering the gut microbiota and intestinal metabolic homeostasis.
    Article Snippet: Background: Antibiotic-associated diarrhea (AAD) has had a significant increase in the last years, with limited available effective therapies.. Shengjiang Xiexin Decoction (SXD), a classic traditional Chinese medicine formula for treating diarrhea, is a promising alternative for reducing the incidence of AAD.. Purpose: This study aimed to explore the therapeutic effect of SXD on AAD and to investigate its potential therapeutic mechanism by integrated analysis of the gut microbiome and intestinal metabolic profile.

    Article Title: Red yeast rice extract’s impact on liver health: a pharmacological and metabolomic exploration
    Article Snippet: .. Then, the sections were incubated with anti-rabbit antibody labeled with secondary antibodies (Goat anti-rabbit IgG H&L(HRP), 1:20000, abcam, Cat. Ab205718 ) for 1 h. The nuclei were visualized by Fluoroshield mounting medium with DAPI (Beyotime Biotechnology, Shanghai, China) staining. .. Immunohistochemistry analysis were performed using Image ProPlus 6.0 software (Media Cybernetics Inc., Silver Spring, MD, United States) for semiquantitative analysis.

    Article Title: Histamine H 1 receptors in dentate gyrus-projecting cholinergic neurons of the medial septum suppress contextual fear retrieval in mice
    Article Snippet: .. After blocking by 5% normal donkey serum in PBS for 1 h at room temperature, sections were incubated with anti-ChAT antibody (Millipore, AB144P, 1:100), anti-c-Fos antibody (Synaptic System, 226004, 1:500), at 4 °C overnight and then with AlexaFluor-conjugated secondary antibody-Donkey anti-Goat Alexa Fluor 488 (Invitrogen, A32814, 1:400) or Goat Anti-Guinea pig Alexa Fluor 647 (Abcam, ab150187, 1:400) at room temperature for 2 h. Fluoroshield TM with DAPI (Beyotime, P0131) was used as a nuclear stain. .. The slices were photographed by Olympus FV3000 confocal microscope and further quantified by FIJI (ImageJ-win64) in a blind manner.

    Labeling:

    Article Title: Shengjiang Xiexin Decoction ameliorates antibiotic-associated diarrhea by altering the gut microbiota and intestinal metabolic homeostasis.
    Article Snippet: Background: Antibiotic-associated diarrhea (AAD) has had a significant increase in the last years, with limited available effective therapies.. Shengjiang Xiexin Decoction (SXD), a classic traditional Chinese medicine formula for treating diarrhea, is a promising alternative for reducing the incidence of AAD.. Purpose: This study aimed to explore the therapeutic effect of SXD on AAD and to investigate its potential therapeutic mechanism by integrated analysis of the gut microbiome and intestinal metabolic profile.

    Article Title: Red yeast rice extract’s impact on liver health: a pharmacological and metabolomic exploration
    Article Snippet: .. Then, the sections were incubated with anti-rabbit antibody labeled with secondary antibodies (Goat anti-rabbit IgG H&L(HRP), 1:20000, abcam, Cat. Ab205718 ) for 1 h. The nuclei were visualized by Fluoroshield mounting medium with DAPI (Beyotime Biotechnology, Shanghai, China) staining. .. Immunohistochemistry analysis were performed using Image ProPlus 6.0 software (Media Cybernetics Inc., Silver Spring, MD, United States) for semiquantitative analysis.

    Staining:

    Article Title: Shengjiang Xiexin Decoction ameliorates antibiotic-associated diarrhea by altering the gut microbiota and intestinal metabolic homeostasis.
    Article Snippet: Background: Antibiotic-associated diarrhea (AAD) has had a significant increase in the last years, with limited available effective therapies.. Shengjiang Xiexin Decoction (SXD), a classic traditional Chinese medicine formula for treating diarrhea, is a promising alternative for reducing the incidence of AAD.. Purpose: This study aimed to explore the therapeutic effect of SXD on AAD and to investigate its potential therapeutic mechanism by integrated analysis of the gut microbiome and intestinal metabolic profile.

    Article Title: Red yeast rice extract’s impact on liver health: a pharmacological and metabolomic exploration
    Article Snippet: .. Then, the sections were incubated with anti-rabbit antibody labeled with secondary antibodies (Goat anti-rabbit IgG H&L(HRP), 1:20000, abcam, Cat. Ab205718 ) for 1 h. The nuclei were visualized by Fluoroshield mounting medium with DAPI (Beyotime Biotechnology, Shanghai, China) staining. .. Immunohistochemistry analysis were performed using Image ProPlus 6.0 software (Media Cybernetics Inc., Silver Spring, MD, United States) for semiquantitative analysis.

    Article Title: Histamine H 1 receptors in dentate gyrus-projecting cholinergic neurons of the medial septum suppress contextual fear retrieval in mice
    Article Snippet: .. After blocking by 5% normal donkey serum in PBS for 1 h at room temperature, sections were incubated with anti-ChAT antibody (Millipore, AB144P, 1:100), anti-c-Fos antibody (Synaptic System, 226004, 1:500), at 4 °C overnight and then with AlexaFluor-conjugated secondary antibody-Donkey anti-Goat Alexa Fluor 488 (Invitrogen, A32814, 1:400) or Goat Anti-Guinea pig Alexa Fluor 647 (Abcam, ab150187, 1:400) at room temperature for 2 h. Fluoroshield TM with DAPI (Beyotime, P0131) was used as a nuclear stain. .. The slices were photographed by Olympus FV3000 confocal microscope and further quantified by FIJI (ImageJ-win64) in a blind manner.

    Blocking Assay:

    Article Title: Histamine H 1 receptors in dentate gyrus-projecting cholinergic neurons of the medial septum suppress contextual fear retrieval in mice
    Article Snippet: .. After blocking by 5% normal donkey serum in PBS for 1 h at room temperature, sections were incubated with anti-ChAT antibody (Millipore, AB144P, 1:100), anti-c-Fos antibody (Synaptic System, 226004, 1:500), at 4 °C overnight and then with AlexaFluor-conjugated secondary antibody-Donkey anti-Goat Alexa Fluor 488 (Invitrogen, A32814, 1:400) or Goat Anti-Guinea pig Alexa Fluor 647 (Abcam, ab150187, 1:400) at room temperature for 2 h. Fluoroshield TM with DAPI (Beyotime, P0131) was used as a nuclear stain. .. The slices were photographed by Olympus FV3000 confocal microscope and further quantified by FIJI (ImageJ-win64) in a blind manner.



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    Progressive sialadenitis in the IL-14αTG mouse model of Sjögren's disease. (A) Submandibular and sublingual glands from 6- and a 12-month-old female IL-14αTG and C57BL/6 mice were subjected to hematoxylin and eosin staining to assess glandular inflammation; scale bar = 1 mm. (B) Immune cell focus surrounding blood vessels and excretory ducts in a 12-month-old IL-14αTG mouse SMG; scale bar = 100 µm. (C) Twelve-month-old and (D) 18-month-old female IL-14αTG mouse SMG and SLG cryosections were subjected to immunofluorescence staining using antibodies against aquaporin 5 (AQP5) acinar cell marker, CD45 pan-immune cell marker, B220 B cell marker, CD3 T cell marker, CD169 macrophage marker, CD11c dendritic cell marker or GL7 germinal center marker with <t>DAPI</t> nuclear counterstain; scale bars = 1 mm and 100 µm (inset).
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    Image Search Results


    Progressive sialadenitis in the IL-14αTG mouse model of Sjögren's disease. (A) Submandibular and sublingual glands from 6- and a 12-month-old female IL-14αTG and C57BL/6 mice were subjected to hematoxylin and eosin staining to assess glandular inflammation; scale bar = 1 mm. (B) Immune cell focus surrounding blood vessels and excretory ducts in a 12-month-old IL-14αTG mouse SMG; scale bar = 100 µm. (C) Twelve-month-old and (D) 18-month-old female IL-14αTG mouse SMG and SLG cryosections were subjected to immunofluorescence staining using antibodies against aquaporin 5 (AQP5) acinar cell marker, CD45 pan-immune cell marker, B220 B cell marker, CD3 T cell marker, CD169 macrophage marker, CD11c dendritic cell marker or GL7 germinal center marker with DAPI nuclear counterstain; scale bars = 1 mm and 100 µm (inset).

    Journal: Frontiers in Dental Medicine

    Article Title: Salivary gland transcriptomic analysis and immunophenotyping in the IL-14α transgenic mouse model of Sjögren's disease

    doi: 10.3389/fdmed.2025.1612522

    Figure Lengend Snippet: Progressive sialadenitis in the IL-14αTG mouse model of Sjögren's disease. (A) Submandibular and sublingual glands from 6- and a 12-month-old female IL-14αTG and C57BL/6 mice were subjected to hematoxylin and eosin staining to assess glandular inflammation; scale bar = 1 mm. (B) Immune cell focus surrounding blood vessels and excretory ducts in a 12-month-old IL-14αTG mouse SMG; scale bar = 100 µm. (C) Twelve-month-old and (D) 18-month-old female IL-14αTG mouse SMG and SLG cryosections were subjected to immunofluorescence staining using antibodies against aquaporin 5 (AQP5) acinar cell marker, CD45 pan-immune cell marker, B220 B cell marker, CD3 T cell marker, CD169 macrophage marker, CD11c dendritic cell marker or GL7 germinal center marker with DAPI nuclear counterstain; scale bars = 1 mm and 100 µm (inset).

    Article Snippet: Slides were then washed in PBS and coverslips mounted using Fluoroshield with DAPI (MilliporeSigma).

    Techniques: Staining, Immunofluorescence, Marker